Sentences with phrase «gene disruption»

Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.

To delineate the role of Emk in development and adult tissues, mice lacking Emk were generated by targeted gene disruption.

We have demonstrated that our liposomes can mediate in vivo target mRNA delivery as well as gene disruption using an entirely RNA - based approach.

In the R5 - ZFN control group, with intact cxcr4 genes, 88 % of CD4 + T cells expressed CXCR4 protein at day 27 post engraftment, compared to 84 % of cells in the X4 - ZFN mice (∼ 24 % cxcr4 gene disruption) as determined by a fluorescence minus - one (FMO) control.

The Drosophila gene disruption project: progress using transposons with distinctive site - specificities.

In the context of epilepsy, he added, de novo mutations seem to involve mechanisms of gene disruption that are unlike those involved in intellectual disability.

Furthermore, gene disruption by gene editing techniques such as CRISPR - CAS resulted in the absence of growth of parasites carrying the mutated gene, indicating that the protein is required for parasite viability.

Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein - coding genes.

As part of its efforts to develop and apply tools for large - scale functional analysis, the BDGP has undertaken a novel gene disruption project based on mutagenesis by transposable element insertion (Spradling et al., 1995).

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.

«Hepatic gene disruption in vivo after liposomal delivery of Cas9 mRNA and chemically modified guide RNAs»

The BDGP gene disruption project: single transposon insertions associated with 40 % of Drosophila genes Bellen, H.J., Levis, R.W., Liao, G., He, Y., Carlson, J.W., Tsang, G., Evans - Holm, M., Hiesinger, P.R., Schulze, K.L., Rubin, G.M., Hoskins, R.A., and Spradling, A.C. Genetics (2004) 167:761 - 781

In addition to genomic sequencing, the BDGP is 1) producing gene disruptions using P element - mediated mutagenesis on a scale unprecedented in metazoans; 2) characterizing the sequence and expression of cDNAs; and 3) developing informatics tools that support the experimental process, identify features of DNA sequence, and allow us to present up - to - date information about the annotated sequence to the research community.

If female fertility gene disruption occurred early in development rather than in the germline, the same system could produce a male - linked dominant sterile - daughter effect that would be less powerful but more readily modulated (Supplementary Fig. 10).

Immune function and pathology dissected by high - throughput analysis of mice with targeted gene disruptions — an investigation by the Infection and Immunity Immunophenotyping (3i) consortium.

Virginia Tech researchers successfully used a gene disruption technique to change the eye color of a mosquito — a critical step toward new genetic strategies aimed at disrupting the transmission of diseases such as dengue fever.

We remain confident that resources, such as the gene disruption library, which integrate molecular and genetic methods will play an increasingly important role in future biological research.

The frequent proximity of the insertions to the 5» portion of the transcription unit means that the gene disruption in many lines could be molecularly identified by comparing the DNA sequence flanking the insertion to genomic sequence data.