Sentences with phrase «gene disruption by»

Furthermore, gene disruption by gene editing techniques such as CRISPR - CAS resulted in the absence of growth of parasites carrying the mutated gene, indicating that the protein is required for parasite viability.

Not exact matches

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Through temporary disruption of these interactions, the scientists were able to produce Drosophila epilines characterized by different levels of PcG - dependent gene repression or activation.

In the R5 - ZFN control group, with intact cxcr4 genes, 88 % of CD4 + T cells expressed CXCR4 protein at day 27 post engraftment, compared to 84 % of cells in the X4 - ZFN mice (∼ 24 % cxcr4 gene disruption) as determined by a fluorescence minus - one (FMO) control.

Immune function and pathology dissected by high - throughput analysis of mice with targeted gene disruptions — an investigation by the Infection and Immunity Immunophenotyping (3i) consortium.

To delineate the role of Emk in development and adult tissues, mice lacking Emk were generated by targeted gene disruption.

Genetic Alterations: Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.

As part of its efforts to develop and apply tools for large - scale functional analysis, the BDGP has undertaken a novel gene disruption project based on mutagenesis by transposable element insertion (Spradling et al., 1995).

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.

The frequent proximity of the insertions to the 5» portion of the transcription unit means that the gene disruption in many lines could be molecularly identified by comparing the DNA sequence flanking the insertion to genomic sequence data.

Following up on findings from genome - wide association studies, researchers identify two mechanisms by which disruptions in the gene SLC16A11 may play a role in type 2 diabetes

We are currently testing the hypothesis that these transcription factors form a genomic regulatory network, and disruption of that network by mutation destabilizes gene expression programs, contributing to malignancy.

Potential projects include identifying common pathways that modify retinal degenerative disease from a large collection of actively maintained mouse models; determining molecular networks implicated in pathological disruption of the retinal pigment epithelium; identifying molecular pathways that regulate postnatal ocular growth; and using mouse models to assess the pathogenic role of gene variants that increase the risk of age - related macular degeneration as identified by human genome - wide association studies.

We have been searching for a mechanism that can explain the genetic cause of majority of BWS and that can provide better understanding of intragenic mutation of p57KIP2 and disruption of KvLQT1 gene by balanced germline chromosomal rearrangements.

Gene disruption in cells and model organisms followed by phenotypic analysis has been a traditional approach to understand gene functGene disruption in cells and model organisms followed by phenotypic analysis has been a traditional approach to understand gene functgene function.