Sentences with phrase «gene disruption in»

Gene disruption in cells and model organisms followed by phenotypic analysis has been a traditional approach to understand gene function.

The frequent proximity of the insertions to the 5» portion of the transcription unit means that the gene disruption in many lines could be molecularly identified by comparing the DNA sequence flanking the insertion to genomic sequence data.

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.

While CXCR4 gene disruption remained stable over time at approximately 30 %, CXCR4 gene disruption in HIV - infected cultures increased to 87 %, 91 %, and 88 % in the presence of BK132, HxB2, and R3A respectively after 21 days of infection.

Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.

Not exact matches

The disruption of prenatal cellular activity in zebra fish, which share 80 percent of their genes with humans and are considered a good model for studying human brain development, seemed to result in hyperactivity, according to the Canadian study, which was published Monday in the Proceedings of the National Academy of Sciences.

To determine if defects in the atrial natriuretic peptide (ANP) system can cause hypertension, mice were generated with a disruption of the proANP gene.

«Our findings show that the gene mutation that causes Werner syndrome results in the disorganization of heterochromatin, and that this disruption of normal DNA packaging is a key driver of aging,» says Juan Carlos Izpisua Belmonte, a senior author on the paper.

Disruptions to one or other of this gene cluster leads to distinctive mutations in which legs develop in place of antennae, for instance, as pictured here.

Furthermore, gene disruption by gene editing techniques such as CRISPR - CAS resulted in the absence of growth of parasites carrying the mutated gene, indicating that the protein is required for parasite viability.

Even clearer evidence that disruptions in gene imprinting can undermine mental health comes from studies of Prader - Willi syndrome, a disorder that affects growth, sexual development and cognitive ability.

Mice generated to contain a targeted disruption of the DARPP - 32 gene showed profound deficits in their molecular, electrophysiological, and behavioral responses to dopamine, drugs of abuse, and antipsychotic medication.

«We identified that the peculiar look of these naked lizards is due to the disruption of the ectodysplasin - A (EDA), a gene whose mutations in humans and mice are known to generate substantial abnormalities in the development of teeth, glands, nails and hairs», says Michel Milinkovitch.

The new finding is the latest evidence supporting a growing precision medicine model of psychiatric disease in which disruptions of certain genes during brain development contribute to a person's risk for multiple psychiatric disorders, with other genetic or epigenetic drivers, random developmental events, or environmental influences determining the specific disease an individual develops, said senior author Benjamin Cheyette, MD, PhD, an associate professor of psychiatry and a member of the UCSF Weill Institute for Neurosciences and the Kavli Institute for Fundamental Neuroscience at UCSF.

Dr. Je and his team analysed signalling activity in neuronal cultures that either did not have the DTNBP1 gene or had lowered levels of the gene, because reduced DTNBP1 levels and genetic disruptions of DTNBP1 in mice resulted in schizophrenia - like behaviours.

«The disruption of the circadian clock machinery seems to affect not only the rhythm of appetite regulation, but also the expression of the genes and proteins at their absolute level,» says Turek, whose team reports its findings online today in Science.

Over the past decade, several dozen genes have been identified as potential culprits, and scientists believe that several genes cause disruptions in protein formations predisposing a person to schizophrenia.

Beverly Emanuel, a human geneticist at the University of Pennsylvania in Philadelphia, notes that some DiGeorge patients have genetic disruptions at the suspect region but seem to have an intact UFD1 gene.

Differences in how genes are controlled, or even the loss or disruption of certain gene regulatory elements, may explain why human ancestors evolved to be so different from their great ape relatives.

In experiments with mice, disruptions in clock genes can lead to obesity, although scientists don't yet understand the mechanisIn experiments with mice, disruptions in clock genes can lead to obesity, although scientists don't yet understand the mechanisin clock genes can lead to obesity, although scientists don't yet understand the mechanism.

To begin to explore the cellular mechanisms that might be responsible for this response, histological analysis revealed less severe disruption of spermatogenesis and gene expression studies suggested that an interplay of ATP - binding cassette (ABC) efflux transporters and solute carrier (SLC) influx transporters in the testicular cells might be involved.

In the R5 - ZFN control group, with intact cxcr4 genes, 88 % of CD4 + T cells expressed CXCR4 protein at day 27 post engraftment, compared to 84 % of cells in the X4 - ZFN mice (∼ 24 % cxcr4 gene disruption) as determined by a fluorescence minus - one (FMO) controIn the R5 - ZFN control group, with intact cxcr4 genes, 88 % of CD4 + T cells expressed CXCR4 protein at day 27 post engraftment, compared to 84 % of cells in the X4 - ZFN mice (∼ 24 % cxcr4 gene disruption) as determined by a fluorescence minus - one (FMO) controin the X4 - ZFN mice (∼ 24 % cxcr4 gene disruption) as determined by a fluorescence minus - one (FMO) control.

To delineate the role of Emk in development and adult tissues, mice lacking Emk were generated by targeted gene disruption.

After acknowledging that there can be complicated interactions between genes, and that DNA insertion might influence regulatory networks involved in many genes, it also should be remembered that the original discovery of genes was only possible because disruption of a gene causes a limited set of changes to the organism, such as a change in eye colour.

A scheme of known expressed isoforms listed in WormBase WS246 and the strategy for the disruption of W01A8.1 gene is shown in Figs.

Targeted disruption of IRF - 1 or IRF - 2 results in abnormal type I IFN gene induction and aberrant lymphocyte development.

Disruptions in autophagy and mutations in related genes are linked to Parkinson's disease, type 2 diabetes and genetic diseases.

Cells rely on these pathways to respond appropriately to their environment (so that the right growth - controlling genes get turned on in response to a growth signal)-- but disruptions in signaling pathways can have dire consequences.

Azoospermia in mice with targeted disruption of the Brek / Lmtk2 (brain - enriched kinase / lemur tyrosine kinase 2) gene.

MEK kinase 1 gene disruption alters cell migration and c - Jun NH2 - terminal kinase regulation but does not cause a measurable defect in NF - $ ąppa $ B activation.

The tools currently available to insert new genes permanently into cells do so at a random site inside the cell, which occasionally results in the disruption of the cell's native genes at the site of insertion, which could potentially turn the cell cancerous or cause other problems.

We remain confident that resources, such as the gene disruption library, which integrate molecular and genetic methods will play an increasingly important role in future biological research.

We have demonstrated that our liposomes can mediate in vivo target mRNA delivery as well as gene disruption using an entirely RNA - based approach.

We interfere with histone modification marks in vivo, both genome - wide (mutant analysis) and gene - specifically (local disruption of chromatin structure), and analyze the effect on transcription and pluripotency.

There was a significant enrichment of gene expression disruption to pathways relevant in oxidative phosphorylation; and carbohydrate, amino acid, and lipid metabolism in diabetic co-twins.

Following up on findings from genome - wide association studies, researchers identify two mechanisms by which disruptions in the gene SLC16A11 may play a role in type 2 diabetes

Scientists have for the first time shown how the disruption of a key gene involved in mental illness impacts on the brain.

Insect Allies» three technical areas — trait design, insect vector optimization, and selective gene therapy in mature plants — layer together to support the goal of rapidly transforming mature plants to protect against agricultural disruption without the need for extensive infrastructure.

Potential projects include identifying common pathways that modify retinal degenerative disease from a large collection of actively maintained mouse models; determining molecular networks implicated in pathological disruption of the retinal pigment epithelium; identifying molecular pathways that regulate postnatal ocular growth; and using mouse models to assess the pathogenic role of gene variants that increase the risk of age - related macular degeneration as identified by human genome - wide association studies.

Overall, these observations provide a roadmap for a «human knockout project», a systematic effort to understand the phenotypic consequences of complete disruption of genes in humans.

If female fertility gene disruption occurred early in development rather than in the germline, the same system could produce a male - linked dominant sterile - daughter effect that would be less powerful but more readily modulated (Supplementary Fig. 10).

In addition to genomic sequencing, the BDGP is 1) producing gene disruptions using P element - mediated mutagenesis on a scale unprecedented in metazoans; 2) characterizing the sequence and expression of cDNAs; and 3) developing informatics tools that support the experimental process, identify features of DNA sequence, and allow us to present up - to - date information about the annotated sequence to the research communitIn addition to genomic sequencing, the BDGP is 1) producing gene disruptions using P element - mediated mutagenesis on a scale unprecedented in metazoans; 2) characterizing the sequence and expression of cDNAs; and 3) developing informatics tools that support the experimental process, identify features of DNA sequence, and allow us to present up - to - date information about the annotated sequence to the research communitin metazoans; 2) characterizing the sequence and expression of cDNAs; and 3) developing informatics tools that support the experimental process, identify features of DNA sequence, and allow us to present up - to - date information about the annotated sequence to the research community.

Bypass of senescence after disruption of p21CIP1 / WAF1 gene in normal diploid human fibroblasts.

Mihail Sarov (Max Planck Institute, Germany): CRISPR / Cas9 - induced disruption of gene expression in mouse embryonic brain

In contrast with the BN rat strain, the disruption of the Themis1 gene in LEW rats does not lead to the spontaneous development of IBD or functional Treg defect, suggesting that an epistasis phenomenon in the BN genetic background controls these phenotypeIn contrast with the BN rat strain, the disruption of the Themis1 gene in LEW rats does not lead to the spontaneous development of IBD or functional Treg defect, suggesting that an epistasis phenomenon in the BN genetic background controls these phenotypein LEW rats does not lead to the spontaneous development of IBD or functional Treg defect, suggesting that an epistasis phenomenon in the BN genetic background controls these phenotypein the BN genetic background controls these phenotypes.

Disruption of the proto - oncogene int - 2 in mouse embryo - derived stem cells: A general strategy for targeting mutations to nonselectable genes.

AAEM pointed to numerous animal studies that showed oxidative stress, altered structure and function of the liver, intestinal damage, disruption of the immune system, increased cell growth and hundreds of alterations in gene expressions.

This happens when there is a mutation of the FGFR3 gene (the gene responsible for producing a protein that develops and maintains the growth of bone and brain tissue), which then causes disruptions in skeletal development.