Sentences with phrase «gene disruption using»

We have demonstrated that our liposomes can mediate in vivo target mRNA delivery as well as gene disruption using an entirely RNA - based approach.

In addition to genomic sequencing, the BDGP is 1) producing gene disruptions using P element - mediated mutagenesis on a scale unprecedented in metazoans; 2) characterizing the sequence and expression of cDNAs; and 3) developing informatics tools that support the experimental process, identify features of DNA sequence, and allow us to present up - to - date information about the annotated sequence to the research community.

Gene Disruptions Using P transposable elements: An integral component of the Drosophila genome project A.C. Spradling et al..

Not exact matches

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein - coding genes.

Virginia Tech researchers successfully used a gene disruption technique to change the eye color of a mosquito — a critical step toward new genetic strategies aimed at disrupting the transmission of diseases such as dengue fever.

Potential projects include identifying common pathways that modify retinal degenerative disease from a large collection of actively maintained mouse models; determining molecular networks implicated in pathological disruption of the retinal pigment epithelium; identifying molecular pathways that regulate postnatal ocular growth; and using mouse models to assess the pathogenic role of gene variants that increase the risk of age - related macular degeneration as identified by human genome - wide association studies.

The Drosophila gene disruption project: progress using transposons with distinctive site - specificities.