Sentences with phrase «gene expression analyses»

Global gene expression analyses reveal changes in biological processes after hyperthermia in a rat glioma model.

Typical microarray - based gene expression analyses compare gene expression in adjacent normal and cancerous tissues.

«Computational method makes gene expression analyses more accurate: Detects technical biases that otherwise confound test results.»

Single - cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.

Gene expression analysis suggests that club cells express high amounts of antiviral genes in response to infection.

Dartmouth Institute for Quantitative Biomedical Sciences (iQBS) researchers developed a new gene expression analysis approach for identifying cancer genes.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

Real - time (quantitative) PCR (qPCR) can overcome these drawbacks and has widespread and proven use in gene expression analysis at both low and medium throughput.

F4 / 80 + and F4 / 80 — cells were collected into cooled FACS buffer, centrifuged at 500 g for 5 minutes, and immediately frozen for gene expression analysis.

Gene expression analysis of the animals» skeletal muscles led to another clue: differences in the kynurenine pathway of tryptophan degradation.

A quest to find good primers for gene expression analysis of Candida albicans from clinical samples — Gabriela C.Alonso — Journal of Microbiological Methods

For gene expression analysis, microarrays were performed by the DKFZ Genomics and Proteomics core facility (Heidelberg, Germany).

We expanded on our genome sequencing efforts to include efforts in whole genome gene expression analysis and functional analysis of genome variation.

The Wnt and TGF - β signaling pathways were identified in the gene expression analysis as being up - regulated during the day (Fig. 4).

Calcification rate measurements and gene expression analysis by microarray RNA transcriptional profiling at two time - points (midday and night - time) revealed several genes common within mammalian gene regulatory networks.

Gene expression analysis was performed by sqPCR or qRT — PCR.

Comparative gene expression analysis revealed a significant overexpression of TRF2, β - catenin and hTERT genes (p < 0.01) in clonospheres at high Bleomycin concentration (80μM)(Fig 2f).

Affymetrix GeneChips are probably the most widely used platform for gene expression analysis.

Whitehead scientists identify major flaw in standard approach to global gene expression analysis

The BioMark Dynamic Array microfluidic qPCR system (Fluidigm Corporation, San Francisco, CA) was used for gene expressions analysis of oocytes and cumulus cells.

Other often utilized technologies are fluorescence activated cell sorting, tissue culture, gene expression analysis, protein analysis, and lentiviral mediated gene transfer.

Analysis of differentially expressed proteins revealed enrichment in cytoskeleton organization, mitochondrial activity and ROS management, while gene expression analysis revealed enrichment in the epithelial - to - mesenchymal transition pathway.

Also, gene expression analysis showed that ERBB2 expression correlates with outcome (higher expression = poor prognosis).

The gene expression analysis suggests that activation of cell - type specific cationic channels may underlie the toxicity of the drug.

In order to validate that the observed cell death was specific to the action of amiodarone HCL, and possibly dissect the mechanism of action of this compound, we performed a gene expression analysis of NSCs and postmitotic neurons receiving amiodarone HCL.

Li W, Turner A, Aggarwal P, Matter A, Storvick E, Arnett DK, Broeckel U. Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis.

Treated and untreated NSCs and neurons were then interrogated with global gene expression analysis to explore the mechanisms of action of amiodarone HCl.

Their gene expression analysis throughout the protocol matched that of a previously published profile for hESCs [24], which included the significant upregulation of mesodermal and chondrogenic markers (PDGFRB, SOX6, SOX9, ACAN, COL2A1 types A and B), with the more mature COL2A1 type B splice variant [42] being more highly expressed by the end of stage 3.

37) Coe BP, Chari R, Lockwood WW, Lam WL (2008) Evolving strategies for global gene expression analysis of cancer.

Therefore, BGEM provides the most comprehensive gene expression analysis of any of the major databases.

They hypothesized that mind - body interventions reverse the expression of genes involved in inflammatory reactions caused by stress, and so they conducted a systematic review of 18 different studies that used gene expression analysis in mind - body interventions.

Feline: Defining Relevant Pathways in Feline Injection - Site Sarcomas with Gene Expression Analysis D08FE - 001, Marlene L. Hauck, Grant Amount: $ 82,123

Tags for this Online Resume: recombinant protein purification, gene expression analysis, Genetic analysis of bacteria

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The potential of genetic and gene expression analysis in the diagnosis of neuropsychiatric disorders.

Not exact matches

In subsequent analyses, striking losses in gene expression were observed in genomic regions that had become increasingly methylated with age, whereas regions that had become less methylated showed increases in gene expression.

Other labs have used DNA microarray analysis to characterize gene expression changes in this model.

A new meta - analysis suggests that low expression levels of 10 related gene sets responsible for mitochondrial machinery play an important role in this disorder — all previously unlinked to Parkinson's.

In a three - stage meta - analysis, Harvard University neurologist Clemens Scherzer and his collaborators analyzed gene expression in 410 samples taken from patients that either had symptomatic or asymptomatic Parkinson's or were healthy, including 185 samples of substantia nigra — a midbrain region where dopamine neurons are particularly susceptible to degeneration.

First, they did a quantitative analysis of the anatomy of related fossils and extant animals to generate a hypothesis about the transition; next, they searched for possible shifts in gene expression that correlated with the transition.

The centre will be structured around six technical platforms focusing on zebrafish transgenesis and micromanipulation, bioimaging, mouse transgenesis, flow cytometry, stem cell culture, and gene - expression analysis.

Scientists from the Biogerontology Research Foundation (BGRF), a UK - based charity founded to support aging research and address the challenges of a rapidly aging population, propose a new concept for signalome - wide analysis of changes in intracellular pathways, called OncoFinder, which allows for accurate and robust cross-platform analysis of gene expression data.

The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type of single - cell gene - expression analysis.

Their study, in the journal Oncotarget, is the most extensive analysis to date of gene expression's role in ccRCC tumor growth and metastasis.

Band led a previous analysis of gene expression in blind mole - rats living in low - oxygen (hypoxic) environments.

«Genome - wide analysis allowed us to identify a gene, VNN - 1, whose expression discriminated between good and poor responders to systemic corticosteroid treatment,» said Gurjit Khurana Hershey, MD, PhD, senior author and director of Asthma Research at Cincinnati Children's.

An analysis of gene expression of the known fungal partner in the two species showed no differences.

Analysis of liver gene expression patterns of the exposed rats found that the endocrine disruptors induced developmental reprogramming of the animals» epigenomes.

Biomolecular model based on the gene expression data analyses support the reduction of glucose molecules (blue gradient) and acid buildup (gold gradient) proposed to occur in the boundary layer around the cell.

In the article titled «Computational Gene Expression Modeling Identifies Salivary Biomarker Analysis that Predicts Oral Feeding Readiness in the Newborn,» the scientists describe their two - phase research.