Sentences with phrase «gene expression in»

Our results reveal the regulatory mechanisms that interplay to drive transcription factor occupancy, chromatin state, and gene expression in complex mammalian cell states.

For instance, a researcher interested in cardiovascular disease could access GTEx data to view all the genetic variants in the human genome that affect gene expression in the heart.

For the analysis of gene expression in blastocysts, total RNA was isolated and purified from three pools of 10 blastocysts produced in vivo or in vitro (KSOM supplemented with 10 % FCS or in the presence of 1 g / L BSA) using the RNeasy Micro Kit (Qiagen)[18].

Single - cell RNA - sequencing (scRNA - seq) allows studying heterogeneity in gene expression in large cell populations.

DNA regions bound at low occupancy by transcription factors do not drive patterned reporter gene expression in Drosophila.

Methods: Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons.

These findings on gene expression in single embryonic stem cells are in concert with recent studies of early mammalian development, which reveal molecular heterogeneity and a stochasticity of gene expression in blastomeres.

We sought first to examine gene expression in single ES cells isolated directly from specific zones within colonies, with two objectives.

Paternally inherited Dp / + (patDp / +) mice showed expected increases in the gene expression in bone, normal postnatal growth and body weight acquisition compared to the littermate controls.

Growth hormone (GH) receptors in prostate cancer: gene expression in human tissues and cell lines and characterization, GH signaling and androgen receptor regulation in LNCaP cells.

In agreement with this, both wt STAT2 and mutant STAT2 - Y690F displayed a similar effect on the luciferase reporter gene expression in U3A cells without IFNα.

The company, intrigued with the results, wanted to know if chromium was affecting gene expression in fat metabolism.

When people eat a plant - heavy diet, the fiber from the plant matter ferments in the gut and creates short - chain fatty acids, which, in turn, regulate the immune system and influence gene expression in the brain and elsewhere.

A comprehensive study of gene expression in tomato fruits will explore unanswered questions about gene regulation, hormonal control and structural polymers that make up cell walls in fruits, to improve crop quality and yields.

Similarly, microarray analyses comparing global gene expression in cells that overexpress wild - type STAT3 or phosphorylation - deficient mutant indicate that a subset of genes are regulated by unphosphorylated STAT3, including well - known oncogenes such as MRAS and MET (Yang et al., 2005).

MicroRNAs MicroRNAs (miRNAs) are short, highly conserved non-coding RNA molecules that repress gene expression in a sequence - dependent manner.

A.H.L.F. investigated musculature development and gene expression in Platynereis.

Sometimes this entails routine evaluation of gene expression in a tissue or organism, or other times high - throughput molecular assays involving thousands of samples.

The Arabidopsis ssi1 Mutation Restores Pathogenesis - Related Gene Expression in npr1 Plants and Renders Defensin Gene Expression Salicylic Acid Dependent

We compared the transcriptomes from VNO and OM of both male and female mice to assess whether differences in gene expression in these tissues could underpin sexually dimorphic behaviors [17].

The Kind group studies the regulation of gene expression in single cells by developing and using novel microscopy and genomics based techniques.

Translational regulation of light - induced ribulose 1,5 - bisphosphate carboxylase gene expression in amaranth

The transcription factors usually function as homodimers or heterodimers to regulate the gene expression in eukaryotic cells and their activities are dependent on distinct protein - protein interaction.

To enable comparison of gene expression in diverse bacterial species in myriad sample types and growth conditions, much of his work is dedicated to developing more robust and high - throughput methods for generating bacterial cDNA libraries and mining bacterial RNA - Seq data for biologically relevant trends.

5/21/2007 Decoding Gene Expression in Cancer Tumors Using Non-Invasive Imaging By correlating images of cancerous liver tissue with gene expression patterns, a research team led by a radiologist at the University of California, San Diego (UCSD) School of Medicine has developed tools that may some day allow physicians to view a... More...

The study found that the PDMP mathematical model accurately describes the random, or stochastic, dynamics of gene expression in the non-adiabatic regime (where promoter kinetics are slow and fast averaging can not occur).

Developing novel tools to quantify gene expression in single cells As it has become increasingly apparent that gene expression in individual cells deviates significantly from the average behavior of cell populations, new methods that provide accurate integer counts of mRNA copy numbers in individual cells are needed.

In addition, he has also discovered another protein that can be affected by drugs and directly influences gene expression in both cancer and heart failure.

- The work, published in Nature Genetics, represents an important advance in our understanding of gene regulation and reveals a new layer of complexity that needs to be studied to properly interpret genomics and gene expression in the future.

These reporters will give us the ability to sort infected cultures to analyze changes in gene expression in directly infected cells and bystander cells and compare to uninfected cells.

Analyzing patterns of gene expression in individual human immune system cells, the researchers refined the definitions of the types known as dendritic cells and monocytes and identified a novel type that had been overlooked.

He is also principal investigator of a pilot study investigating neuroimaging and gene expression in patients with catatonia.

Monitor gene expression in the developing pancreas by placing FP timer DsRed - E5 under the control of

Inhibition of FGF signalling with PD173074 (10 nM) increases the levels of Nanog gene expression in two HV clones while slightly reducing low - level Gata6 expression.

Transcriptional and post-transcriptional regulation of ribulose 1,5 - bisphosphate carboxylase gene expression in light - and dark - grown amaranth cotyledons

The Kind group uses these techniques to study the role of genome architecture and chromatin context in temporal and spatial control of gene expression in development and disease.

For analyses of gene expression in embryos, cryopreserved day 3 embryos and day 6 blastocysts from 10 IVF / ICSI patients were thawed via an embryo thawing kit (Cooper Surgical) or a blastocyst thawing kit (Cooper Surgical), respectively, according to manufacturer's protocol.

The scientists think that telomere length affects disease severity by changing gene expression in pathways implicated in CAVD, such as anti-inflammatory and anti-calcifications pathways.

(C) HMOX1 gene expression in NHLF cells treated with Nrf2 siRNA.

The finding is a landmark in the new field of evolutionary developmental biology, or «evo - devo,» as its proponents call it, in which scientists study the patterns of gene expression in embryos to peer backward in time.

Research Focus: I dedicate my time and effort to develop high - throughput sequencing technologies such as single cell transcriptomic, ChIP - Seq, ChIA - PET, and many more... The scientific rational is to understand better and with a different angle, the mechanisms of epigenetic regulation of gene expression in (rare) immune cells, pathologically relevant in many diseases such as asthma, SLE, tuberculosis...

One study of gene expression in peripheral blood mononuclear cells indicated that B cells might be altered in CFS [14].

Similarly, genetic silencing of Keap1 is insufficient to maximally activate HMOX1 gene expression in Keap1 null mice [52].

These GEMM - ESCs, with often multiple modified alleles, form the basis for further genetic engineering either by Flp - recombinase mediated integration, gene targeting or Crispr / Cas9 to allow for the evaluation of altered target gene expression in a spontaneous tumor model.

Using a specialized DNA array containing thousands of Arabidopsis genes (an EST microarray), the researchers then analyzed gene expression in their mutant and quickly found that MPK4 somehow controls the expression of anti-microbial genes of the SAR response.

CLEMSON — Three Clemson University scientists have been awarded a $ 1.8 million grant to study gene expression in roots that eventually could pave the way for increased crop production, save the world billions of dollars in fertilizer usage and help rid the planet of a damaging pollutant.

Analysis of synaptic gene expression in the neocortex of primates reveals evolutionary changes in glutamatergic neurotransmission.

Using novel gene - array technology to measure the extent of gene expression in thousands of genes simultaneously, this study shows that as humans diverged from their ape ancestors in the last five million years, genes for transcription factors — which control the expression of other genes — were four times as likely to have changed their own expression patterns as the genes they regulate.

Crystal structure of a Cas9 in complex with an RNA guide and a stretch of target DNAWIKIMEDIA, H. NISHIMASU ET AL.. A team of researchers at the Gladstone Institutes in San Francisco, California, has used a version of CRISPR gene editing known as CRISPR interference (CRISPRi) to reversibly and accurately suppress gene expression in induced pluripotent stem cells (iPSCs) and derivative T cells and heart cells, according to a study published in Cell Stem Cell today (March 10).

«We responded to this challenge by proposing to engineer maize viruses, aphids, and leafhoppers such that they can be deployed rapidly to change gene expression in mature maize plants.»