Sentences with phrase «gene fragment into»
The bacterial expression vector pGEX - 5 × -1 / Sp1 was constructed by introducing an EcoRI / XhoI — treated Sp1 gene fragment into EcoRI / XhoI — treated pGEX - 5 × -1 vector.
http://cancerres.aacrjournals.org/ Not exact matches
In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.
The Tetrahymena intervening sequence (IVS) has been inserted into the gene for the alpha - donor fragment of beta - galactosidase in a recombinant plasmid.
Next, they put the samples into an instrument called a DNA sequencer — which automatically analyzes genetic material — to read short viral gene fragments millions or even billions of times.
Finally, small beads are used to sort the mixture of DNA fragments into the right combinations to make longer genes, and the sections are combined.
Using a slew of molecular techniques, the team identified the color - stealing culprit in white grapes as a mobile fragment of DNA, called a retrotransposon, which inserts itself into the gene controlling pigment production.
To look for genes that might control genetic instability, the researchers added a small but toxic fragment of the Huntington's disease gene into two different strains of mice.
The mammalian expression vector pcDNA - SRα / FLAG - DDX 3 was constructed by insertion of a 2 - kb EcoRI / ApaI — treated fragment of the DDX3 gene derived by reverse transcription - PCR (RT - PCR) into EcoRI / ApaI — treated pcDNA - SRα / FLAG vector.
Some of the team's most significant findings since 2009 were the identification of a gene mutation associated with blood cancers and the discovery of chromosomal crisis where the genome can be shattered into hundreds of fragments in a single cellular catastrophe.
The plasmids targeting each gene were respectively transfected into goat fibroblasts, and their genome modification efficiency was determined at day 3 using restriction fragment length polymorphism (RFLP) assay (see Methods).
To compare the expression values across genes and conditions, raw count data was transformed into fragments per kilobase of exon per million fragments (FPKM) with the formula:
The modern human ancestor who contributed genes to this particular Neanderthal individual — called the «Altai Neanderthal,» and known from a tiny toe bone fragment — must have migrated out of Africa long before the migration that led Africans into Europe and Asia 60,000 years ago, the scientists say.
It acts as a caspase inhibitor, which means it could prevent the dangerous huntingtin protein from being broken into small enough fragments to enter the cell's nucleus and mess up the switching on and off of important genes.
Unlike typical eukaryotic organisms, Tetrahymena has two nuclei — a micronucleus that contains normal chromosomes and a macronucleus whose chromosomes are fragmented into thousands of small pieces of DNA that all encode the same ribosomal RNA gene.