Sentences with phrase «gene knockout mice»

An international team of researchers has identified a cause for chronic bad breath (halitosis), with the help of gene knockout mice from the UC Davis Mouse Biology Program.

In this study, by using a systemic mtEF4 gene knockout mouse model, researchers found that mtEF4 knockout damages the oxidative phosphorylation function in germ cells of male mice, thus causing male sterility.

Not exact matches

The knockout mice, which will be made available to academic researchers at low cost, should help efforts to figure out what newly discovered genes do in the body.

Looking forward, the researchers plan to investigate knockout mice lacking the gene that produces AGF to further define its role.

Knockout mice, which completely lacked the gene and so manufactured no mu opiate receptors, were all the more sensitive.

To find out what BRCA2 does normally, the team reporting the new results, which includes Allan Bradley of Baylor College of Medicine in Houston and Paul Hasty of Lexicon Genetics Inc. in The Woodlands, Texas, created «knockout» mice in which the gene was inactivated.

Because mice lacking both genes would not be born alive, the scientists followed up this lead by making «conditional knockout mice,» in which Esrp1 and Esrp2 activity was normal early in fetal development, but then was switched off in skin epithelial cells.

The researchers bred two types of FOXP2 «knockout» mice: One group was homozygous, meaning the mice carried two copies of the disrupted gene; the second group was heterozygous, with one functional and one defective gene.

In an attempt to increase sensitivity for sepsis, Canaan aged these FAT10 knockout mice and made the discovery that mice lacking the gene were lean and aged more slowly.

The scientific community uses knockout mice to analyze basal phenotypes (the appearance of genetic characteristics) to analyze the function of such a large number of genes.

There is a new contender in the field of gene discovery, and it's giving knockout mice a run for their money.

Eliminating genes in mice is a standard technique for determining their function; the resultant animals are called knockout mice.

Now that they have a FAN1 knockout mouse in hand, and they have confirmed that it mimics many aspects of karyomegalic interstitial nephritis in humans, Smogorzewska's lab can start to delve into how the loss of the gene causes kidney degeneration.

Wanting to understand the relationship between CMG2 and collagen VI, the team developed double - knockout mice in which the genes for both proteins — CMG2 and collagen VI — had been deleted.

The FAN1 knockout mice that Smogorzewska's lab has generated allow them to investigate the role of FAN1 in DNA repair, and to figure out how mutations in the gene may lead to kidney disease.

The first group was mice with targeted deletion of the interleukin 10 (IL10) gene, known as interleukin 10 knockout (IL10 - / --RRB- mice, which develop colitis through T helper immune cells.

The researchers at Bonn University and their colleagues from the Federal Institute of Drugs and Medical Devices (BfArM) and from the University of Regensburg worked with so - called transgenic and knockout mice in whom the gene for micro-RNA 155 was either increased or silenced.

In other research, a knockout of the gene that encodes one type of lncRNA in mice conferred some resistance to obesity caused by a high - fat diet.

Induction of the immediate - early gene c - fos was observed after a nighttime light pulse in both wild - type and knockout mice.

Instead of using the traditional method of introducing genetic mutations into mice to create «knockout» mice — or animals with customized genes — they found they were able to find naturally occurring variants among animals in the wild.

As in earlier studies, the investigators, led by Ren Hen of Columbia University, first created a line of so - called knockout mice that lacked the gene encoding the receptor protein.

When they compared the results to the Mouse Genomic Informatics Knockout database they found 123 genes that corresponded with functional annotation of abnormal extraembryonic tissue morphology, 121 associated with embryonic lethality, and 14 with abnormal embryo implantation.

The mouse embryonic stem cell knockout resource provides a basis for the characterization of relationships between genes and phenotypes.

The goal of the International Mouse Phenotyping Consortium (IMPC) is to discover functional insight for every gene by generating and systematically phenotyping 20,000 knockout mouse strMouse Phenotyping Consortium (IMPC) is to discover functional insight for every gene by generating and systematically phenotyping 20,000 knockout mouse strmouse strains.

Researchers make knockout mice by disrupting the function of individual genes across the animal's genome.

To test their reasoning they conducted a second experiment by crossing mice carrying the prostate - specific IGF - 1R knockout alleles with transgenic mice that develop spontaneous prostate cancer when p53 and select other genes are compromised.

Knockout mice provide one of the quickest, most cost effective ways to explore gene function.

In partnership with several international programs, the initial five - year phase of KOMP will reach its goal of creating knockout mouse embryonic stem cell lines for each of the approximately 21,000 protein - coding genes in the mouse genome this year.

The IMPC is generating a knockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse Consortiumknockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse Consortium (Imouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse ConsortiumKnockout Mouse Consortium (IMouse Consortium (IKMC).

These knockout mice, together with the wealth of associated data, will serve as a valuable, freely available, resource for French researchers investigating gene function and human pathophysiology.

Under the grant, Lexicon will utilize its proprietary gene knockout technology to identify genes that, when knocked out, lead to increased levels of mouse Smn protein.

Knockouts of this gene in mice produced animals with defects in memory, behavior, and reward.

Knockout mice are produced by a technique called «gene targeting».

Meehan et al., (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

As expected, tissue studies of the δ - sarcoglycan knockout mice revealed a distinctively different pattern of muscle damage when compared to the damage the researchers found in knockout mice missing the α - sarcoglycan gene.

The process used by NIH to select the mouse lines involved a rigorous scientific review process that evaluated information on the knocked out gene, the reliability of the method used to produce the knockout, and whether the mouse line possesses a «reporter» gene, which enables researchers to analyze the pattern of the knockout gene's expression in various mouse tissues.

To correct RPE65 mutation damage, scientists are evaluating drugs, cell transplants and gene delivery in Rpe65 knockout, transgenic and spontaneous mutant mice, and spontaneous Rpe65 - mutant dogs.

TRPV1: Gene knockout of the pain receptor TRPV1 is one of a number of methods of slowing aging and extending life in mice that appears to work through altered insulin signaling.

Sequencing of the mouse genome coupled with the successful development of transgenic and knockout mouse lines allows researchers to investigate the roles that individual genes play within specific mechanisms of renal physiology under both normal and pathologic conditions (14).

In a series of related studies, the scientists wanted to see what impact vitamin A would have on «knockout mice» missing a gene called sonic hedgehog or Shh.

In these mouse lines, gene knockout can be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP - FRT.

We use what are called conditional knockout mice, in which a gene is deleted only in a specific organ or group of cells.

Technologies / expertise in our laboratory include: quantitative RT - PCR, expression profiling, generation of knockout mice, phylogenetic analysis, serial analysis of gene expression (SAGE), Protein biochemistry, Fluorescence labelling, and Transport kinetics.

The IMPC builds on the efforts of IKMC to produce knockout mice and carry out high - throughput phenotyping of each line in order to determine the function of every gene in the mouse genome.

Projects restricted to the creation of conventional mouse knockouts in candidate disease genes identified by association studies, or to broadly overexpress those genes, are discouraged.

From 2012 onwards the IMPC is continuing the task to generate knockout mice and phenotype the remainder of the 20,000 plus genes in a worldwide coordinated initiative.

WFSM establishes laboratory to develop transgenic and knockout mice to model human disease and shed light on function of human genes.

Yeast, bacteria, worms, and flies had long been used for gene knockout studies, and mice with knockout genes had just been developed before Waldman began his experiments.

Awareness about 2 important publications from IMPC consortium 1 - The analysis of IMPC database that uncovers 360 new human disease models has just been published: Meehan et al., (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

In Aim 1, we generate cell specific - knockout mice and characterize deletion of expression of distinct genes relevant to axon guidance.