Sentences with phrase «gene knockouts by»

Awareness about 2 important publications from IMPC consortium 1 - The analysis of IMPC database that uncovers 360 new human disease models has just been published: Meehan et al., (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

Meehan et al., (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

Not exact matches

Because mice lacking both genes would not be born alive, the scientists followed up this lead by making «conditional knockout mice,» in which Esrp1 and Esrp2 activity was normal early in fetal development, but then was switched off in skin epithelial cells.

In this study, by using a systemic mtEF4 gene knockout mouse model, researchers found that mtEF4 knockout damages the oxidative phosphorylation function in germ cells of male mice, thus causing male sterility.

«Gene knockout: Loss of a gene can be compensated by another gene: Effects of genome interventions depend on the methods used.&raGene knockout: Loss of a gene can be compensated by another gene: Effects of genome interventions depend on the methods used.&ragene can be compensated by another gene: Effects of genome interventions depend on the methods used.&ragene: Effects of genome interventions depend on the methods used.»

In other research, a knockout of the gene that encodes one type of lncRNA in mice conferred some resistance to obesity caused by a high - fat diet.

As in earlier studies, the investigators, led by Ren Hen of Columbia University, first created a line of so - called knockout mice that lacked the gene encoding the receptor protein.

By inducing a stable, complete knockout of specific genes, CRISPR - Cas9 may offer a superior means of identifying genes representing attractive therapeutic targets.

The goal of the International Mouse Phenotyping Consortium (IMPC) is to discover functional insight for every gene by generating and systematically phenotyping 20,000 knockout mouse strains.

Researchers make knockout mice by disrupting the function of individual genes across the animal's genome.

To test their reasoning they conducted a second experiment by crossing mice carrying the prostate - specific IGF - 1R knockout alleles with transgenic mice that develop spontaneous prostate cancer when p53 and select other genes are compromised.

As a result of a number of natural animal lineages with this mutation, myostatin knockout is by far the most examined and tested of all potential gene therapies.

The IMPC is generating a knockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse Consortiumknockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse ConsortiumKnockout Mouse Consortium (IKMC).

Knockout mice are produced by a technique called «gene targeting».

The process used by NIH to select the mouse lines involved a rigorous scientific review process that evaluated information on the knocked out gene, the reliability of the method used to produce the knockout, and whether the mouse line possesses a «reporter» gene, which enables researchers to analyze the pattern of the knockout gene's expression in various mouse tissues.

Programmable nucleases, ZFN, TALEN and RGENs enable gene knockout in cultured cells and organisms by producing site - specific DNA double - strand breaks, whose repair via error - prone non-homologous end joining (NHEJ) or microhomology - mediated end joining (MMEJ) gives rise to frameshift mutations.

In these mouse lines, gene knockout can be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP - FRT.

Projects restricted to the creation of conventional mouse knockouts in candidate disease genes identified by association studies, or to broadly overexpress those genes, are discouraged.

Recently, Hai et al. reported generation of single - gene knockout pigs by zygote injection of CRISPR / Cas system [13].

The Comparative Mouse Genomics Centers Consortium (CMGCC) was initiated by the National Institute of Environmental Health Sciences» (NIEHS) Environmental Genome Project to develop transgenic and knockout mouse models based on human DNA sequence variants in environmentally responsive genes.

To address this challenge, the International Mouse Phenotyping Consortium is creating a genome - and phenome - wide catalog of gene function by characterizing new knockout - mouse strains across diverse biological systems through a broad set of standardized phenotyping tests.

We find that Polycomb repressive complex - active genes have greater cell - to - cell variation in expression than active genes, and these results are validated by knockout experiments.

The first knockout mice constructed by gene targeting were published in 1989, and the rest is history.

Indeed, several lines of evidence suggest that cytokines are associated with animal and human aggression [102]--[104] and IL - 6 was causally linked to aggression in mice by gene knockout evidence [105].