Sentences with phrase «gene knockouts using»

Kohli, Manu, et al. «Facile methods for generating human somatic cell gene knockouts using recombinant adeno ‐ associated viruses.»

«Gene knockout using new CRISPR tool makes mosquitoes highly resistant to malaria parasite: Study highlights the potential of mosquito gene - modification to combat malaria.»

Not exact matches

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the targKnockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the targknockout of the target gene.

The scientific community uses knockout mice to analyze basal phenotypes (the appearance of genetic characteristics) to analyze the function of such a large number of genes.

In this study, by using a systemic mtEF4 gene knockout mouse model, researchers found that mtEF4 knockout damages the oxidative phosphorylation function in germ cells of male mice, thus causing male sterility.

«Gene knockout: Loss of a gene can be compensated by another gene: Effects of genome interventions depend on the methods used.&raGene knockout: Loss of a gene can be compensated by another gene: Effects of genome interventions depend on the methods used.&ragene can be compensated by another gene: Effects of genome interventions depend on the methods used.&ragene: Effects of genome interventions depend on the methods used.»

Instead of using the traditional method of introducing genetic mutations into mice to create «knockout» mice — or animals with customized genes — they found they were able to find naturally occurring variants among animals in the wild.

We use transgenes and gene knockouts to study regulatory processes in vivo.

Researchers typically use viruses for two types of studies --(1) knock - in; introducing a protein - coding gene into cells to study its function, or (2) knockout / knockdown; studying gene function through deletion (potentially using CRISPR lentivirus knockout libraries) or reduction of gene expression, respectively.

The IMPC is generating a knockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse Consortiumknockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse ConsortiumKnockout Mouse Consortium (IKMC).

Workflow for targeted knockout of CD81, an endogenous gene in hiPS cells, using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

The process used by NIH to select the mouse lines involved a rigorous scientific review process that evaluated information on the knocked out gene, the reliability of the method used to produce the knockout, and whether the mouse line possesses a «reporter» gene, which enables researchers to analyze the pattern of the knockout gene's expression in various mouse tissues.

We use what are called conditional knockout mice, in which a gene is deleted only in a specific organ or group of cells.

Cas9 nucleases from Streptococcus pyogenes (SpCas9) have been widely used for both gene knockout and knock - in at the level of single or multiple genes.

Yeast, bacteria, worms, and flies had long been used for gene knockout studies, and mice with knockout genes had just been developed before Waldman began his experiments.

Citation: Ni W, Qiao J, Hu S, Zhao X, Regouski M, Yang M, et al. (2014) Efficient Gene Knockout in Goats Using CRISPR / Cas9 System.

The single - gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in live - born goats harboring biallelic mutations.

Using the two computer equipment, the team found that the knockout mice have seven genes that are vital to the increase and decrease of sleep duration.

Using CRISPR / Cas9 technology, we generated biallelic knockout mutations in host genes encoding IRF5 and IL - 10RA in iPSCs, and confirmed their roles in limiting chlamydial infection in macrophages.

Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knockouts / knock - ins, and target validation using the most current technologies.