The researchers ended up with 172 drug -
gene mutation combinations that successfully killed both yeast and human cancer cells.
Not exact matches
The new study shows that in AML, every possible
combination of homozygous and heterozygous
mutation occurs for the two
gene mutations under study.
The genomic results confirmed Ata's Chilean descent and turned up a slew of
mutations in seven
genes that separately or in
combinations contribute to various bone deformities, facial malformations or skeletal dysplasia, more commonly known as dwarfism.
However, the downside comes when the inevitable accumulation of
mutations takes its toll, or changes to the environment make
gene combinations less well suited than they had once been.
Using a novel
combination of technologies, including trio exome sequencing of patient / parental DNA and genetic studies in the tiny larvae of zebrafish, the EuroEPINOMICS RES consortium found that
mutations in the
gene CHD2 are responsible for a subset of epilepsy patients with symptoms similar to Dravet syndrome — a severe form of childhood epilepsy that is in many patients resistant to currently available anti-epileptic drugs.
They found that while all African mole rats share some
mutations in the HAS2
gene, the naked mole - rat has a unique
combination.
Earlier this year a different research group discovered
mutations in the
gene kelch13 which were linked to reduced susceptibility to artemisinin
combination treatment in South East Asia.
In an effort to expand the number of cancer
gene mutations that can be specifically targeted with personalized therapies, researchers at University of California San Diego School of Medicine and Moores Cancer Center looked for
combinations of mutated
genes and drugs that together kill cancer cells.
To do this, they deleted each
gene one at a time, in
combination with another
mutation.
Fifty - four per cent were single
gene mutations, and the other 13 per cent were polygenic DNA variants, meaning they were a
combination of multiple bad
genes inherited together.
The new
combination therapy is indicated for melanoma patients whose tumors express
gene mutations called BRAF V600E and V600K.
By focusing on several of these
mutations and screening through guide RNA variants with different mismatch
combinations, they were able to identify specific guide RNAs that stimulated Cas9 activity towards the mutated
gene sequences but left the normal counterpart untouched.
Most interestingly, the scientists found that individuals with a particular
combination of
mutations in both
genes had five times the risk of developing asthma than those without.
Analysis of genomic, epigenetic, and RNA sequencing data revealed that the
combinations of
mutations that lowered the levels of functioning BRCA1 and BRCA2 RNA —
genes that produce the breast cancer tumor suppressor proteins — were associated with significantly better survival outcomes.
Since around half the cases of malignant melanoma are driven by
mutation in the BRAF
gene, the team focused on
combinations that might address intrinsic resistance to the BRAF inhibitor vemurafenib.
He and others have recently used
gene - editing tools to correct
mutations in human iPS cells but haven't used the same
combination of methods or done the same set of experiments to determine whether the iPS - derived cells are safe.
While we had previously found an effective treatment for those with the «celtic
gene» this new
combination treatment has the potential to help roughly half of those with cystic fibrosis, those who have two copies the F508DEL
mutation.
Since our collection of cell lines is completely genetically annotated — which means that
mutations and expression changes in each line's
genes have been documented — we should be able to identify in advance patients who will benefit from specific
combinations.
For extended modules, many different
combinations of perturbed
genes could incapacitate the module, as a result of which
mutations in different
genes will appear to lead to the same phenotype (e.g., hypertrophic cardiomyopathy).
One commonly held theory is that autism results from the chance
combinations of commonly occurring
gene mutations, which are otherwise harmless.
This study is intended to evaluate whether the
combinations of these drugs are effective in the treatment of 2 different types of metastatic melanomas depending on whether there is a
mutation of the BRAF
gene.
We conclude that the large number of mouse mutants and human de novo
mutations may be due to the
combination of the Chd7
gene being a large target and the fact that many heterozygous carriers of the
mutations are viable individuals with a readily detectable phenotype.
They identified several causal variations in CCDC115
gene: a homozygous missense
mutation, c. 92T > C (p.Leu31Ser) and this one was also compound heterozygous in
combination with a heterozygous deletion of CCDC115 (in one family) and a homozygous missense
mutation, c. 31G > T (p.Asp11Tyr).
Invivoscribe's clinical laboratories also offer comprehensive MyAML ®, MyHeme ®, MyMRD ®, and custom
gene panels, that when used in
combination with Invivoscribe's proprietary MyInformatics ® Software can identify and track primary driver
mutations as well as the subclonal architecture and emergence of new driver
mutations in patients with hematologic disease.
This is in accordance with previous reports that decitabine and 5 - azacytidine produce a marked synergistic effect in
combination with suberoylanilide hydroxamic acid and romidepsin in T - lymphoma cell lines by modulating cell cycle arrest and apoptosis.26, 27 As a mechanism of action, KMT2D
mutations of B - lymphoma cells promote malignant outgrowth by perturbing methylation of H3K4 that affect the JAK - STAT, Toll - like receptor, or B - cell receptor pathway.28, 29 Here our study indicated that dual treatment with chidamide and decitabine enhanced the interaction of KMT2D with the transcription factor PU.1, thereby inactivating the H3K4me - associated signaling pathway MAPK, which is constitutively activated in T - cell lymphoma.13, 30,31 The transcription factor PU.1 is involved in the development of all hematopoietic lineages32 and regulates lymphoid cell growth and transformation.33 Aberrant PU.1 expression promotes acute myeloid leukemia and is related to the pathogenesis of multiple myeloma via the MAPK pathway.34, 35 On the other hand, PU.1 is also shown to interact with chromatin remodeler and DNA methyltransferease to control hematopoiesis and suppress leukemia.36 Our data thus suggested that the combined action of chidamide and decitabine may interfere with the differentiation and / or viability of PTCL - NOS through a PU.1 - dependent
gene expression program.
ii) HNPCC: germ line inactivation of one allele of either of the mismatch repair
genes MSH2 or MLH1 in
combination with somatic inactivation of the other allele leads to an increase in the
mutation rate, which in turn speeds up the adenoma to carcinoma progression.
Invivoscribe also offers comprehensive MyAML ®, MyHeme ®, MyMRD ®, and custom
gene panels, that when used in
combination with Invivoscribe's proprietary MyInformatics ® Software can identify and track primary driver
mutations as well as the subclonal architecture and emergence of new driver
mutations in patients with hematologic disease.
Using these molecular tools, it has become evident that leukemias, lymphomas and hematologic diseases are characterized by a remarkable genetic heterogeneity, with individual patients presenting with a distinct and almost unique
combination of chromosome changes and somatically acquired
gene mutations.
The most common forms of MTHFR
mutation involve various
combinations of these
genes being passed on from each parent: