Sentences with phrase «gene promoter region»

Early life adversity and serotonin transporter gene variation interact to affect DNA methylation of the corticotropin - releasing factor gene promoter region in the adult rat brain

We tested the hypothesis that a repeat polymorphism in the rhesus macaque MAOA gene promoter region influences aggressive behavior in male subjects.

Dwarki and Jaime Escobedo improved the AAV's ability to insert genes into chromosomes by adding a gene promoter region from cytomegalovirus, known to be active in the target for their gene therapy, muscle cells.

«You don't want to just turn down methylation globally, which would result in over-activation of all genes in the cell, but demethylating some of these gene promoter regions selectively could revive an immune system muted by cancer - causing viruses,» Kuss - Duerkop says.

PULLMAN — Researchers at Washington State University have identified a new class of DNA sequence variation in gene promoter regions that could help control the activity of genes.The novel variations, dubbed «multiple nucleotide length polymorphisms,» or MNLPs, altered transcription of the genes they were associated with as much as 11-fold.

Not exact matches

The pilot project tested a dozen or so of the most commonly used gene promoters (regions of DNA that facilitate gene transcription) and segments of DNA that encode ribosome - binding sites (sequences of messenger RNA that control protein translation) to determine whether they behave consistently in different cellular contexts.

This molecular complex then binds to the promoter region of a gene called Bmi1, an event that increases the expression of the gene.

Specifically, while 5mC is found at repetitive regions of the genome, the methylated adenines were clustered into dense «methylated adenine clusters» (MACs) at gene promoters.

Think of these promoter regions like on - off switches for next - door genes — when a promoter region is methylated, the switch is turned off and the gene it controls does not get read and expressed.

Maybe if virus - related cancers have methylated DNA promoter regions of immune - related genes, the answer to increasing the effectiveness of immune - based therapies against cancer is to demethylate these genes.

The researchers found that MYC may affect circadian rhythm and metabolism by promiscuously binding to promoter regions in key genes for maintaining these daily cycles.

«We found that the Tcl1 gene was upregulated by KLF4 binding to its enhancer and promoter regions,» study co-first author Ken Nishimura says.

A functional polymorphism in the promoter region of the human serotonin transporter gene (SLC6A4) has been associated with several dimensions of neuroticism and psychopathology, especially anxiety traits, but the predictive value of this genotype against these complex behaviors has been inconsistent.

The three stages of CD8 + T cell development are well known, but the current study identifies a detailed map of the regulatory circuitry, such as interactions between enhancers and promoters — genetic regulatory regions that function together in driving genes to transcribe proteins to carry out biological processes.

In particular, they examined different forms of the promoter region of the vasopressin V1a receptor gene (AVPRIA), known as DupA and DupB.

They then asked why biglycan expression was increased in the endothelial cells, and found that the promoter region of the gene was de-methylated, meaning the gene was switched on.

Instead, the differences appear to be in the promoter regions for these genes, an area of DNA close to the gene that helps control whether it ultimately used to make protein.

Previous work had shown that the DNA region in question, a promoter located on chromosome 17, switches on a nearby gene coding for a protein that shuttles serotonin back into nerve cells so they can reuse it as a neurotransmitter.

These sequences in our DNA are often situated far away from the actual genes they regulate, in comparison to so called promoter regions, which traditionally have been considered to control most of the gene activity.

How cells ensure appropriate access to «promoter» DNA, the regions where gene transcription begins, is still poorly understood.

When a given gene needs to be transcribed to create new proteins, its promoter region must be unwrapped from the nucleosome so that it can be accessed by the factors involved in initiating the transcription process.

Interestingly, the results of our promoter analysis for all these genes revealed that 9 of 17 genes (COL13A1, CSF3, FSCN1, HG18, LTBP1, PELI1, PLOD2, SCNN1A, and TNFSF10) have perfectly matched consensus sequences of KLF4 binding site on the promoter regions, suggesting a potential possibility of controlling these genes by KLF4 although it is not clear whether these genes are cause or consequence of brain metastasis at this point.

Comparative analysis of length polymorphisms in the promoter region of the serotonin transporter gene (SLC6A4) in Cercopithecidae [abstract].

For example, a particular gene variant in the promoter region of the IL10 gene is associated with a 40 to 60 % increased risk of developing active tuberculosis among Europeans and Americans [75].

Andy Peters and Ursula Storb show that the initiation of Ig gene transcription targets Ig gene somatic hypermutation (SHM), by duplicating the variable (V) region promoter upstream of the constant (C) region and showing that a second wave of mutations occurs over the C region.

During inflammation reduced levels of NAD + do not impaired the hyperacetylation of NF - κB which in turn through promoter region of target genes activate pro-inflammatory pathway and senescence.

In fact, it was found an association of SIRT6 in some promoter region of NF - kB targeted gene after TNF - alpha stimulation 34.

The MNLPs were discovered within the promoter regions of two genes that belong to the CRH, or corticotropin - releasing hormone family of peptides.

While SIRT1 and 2 inhibit NF - kB through posttranslational modification, SIRT6 neutralizes it by decreasing the accessibility of p65 subunit on promoter region of NF - kB target genes 32.

We searched for over-representation of DNA binding motifs in promoter regions of the 197 differentially expressed genes presented in Supplementary Table 3 using Pscan (28) and the JASPAR database (29).

Sp1 binds to the GC - rich sequence on the proximal region of p21waf1 / cip1 promoter and acts as a mediator for the induction of p21waf1 / cip1 gene caused by transforming growth factor β (TGF - β; ref.

Methylation sites showing inverse relationships with expression were over-represented in promoter regions, whereas methylation sites showing positive relationships with expression were over-represented in gene bodies and 3 ′ UTRs (Fig. 3G and H), which is in agreement with the current literature (39).

Although the researchers found no change in the coding region of the gene, they did find that autistic children were much more likely to have inherited a shortened form of the genes promoter — the DNA sequence that serves as the on ramp for the cellular machinery that will express the gene.

The Maize Mitochondrial cox2 Gene Has Five Promoters in Two Genomic Regions, Including a Complex Promoter Consisting of Seven Overlapping Units

In mouse embryo fibroblasts (MEFs)[2], as well as in the PCa cell lines [unpublished data], DAXX represses expression of these genes via mechanisms that include its binding to DNA methyl transferases (DNMTases) and hypermethylation of the promoter regions of the corresponding genes [2].

It consists of a promoter and an operator; a promoter is the region of the operon that acts as an initial binding site during gene transcription.

In this study, we provide the first evidence that in STAT1 - deficient U3A cells, STAT2 forms a complex with IRF - 9 on the ISRE regions of RIG - G promoter and effectively mediates the transcription of RIG - G gene, even without the tyrosine phosphorylation.

Primers specific for the hygromycin resistance gene were used alongside control primers to sites in the Hex promoter region.

Identification of a novel sequence element in the common promoter region of human collagen type IV genes, involved in the regulation of divergent transcription

It matters, as far as gene expression is concerned, only if it occurs in a «promoter» region, a sequence of DNA needed to turn a gene on or off.

Furthermore, chromatin contact maps that link enhancers to promoter regions in a direct way allow identification of genes that can be regulated by the disease variants.

Linking such regulatory regions to gene promoters in disease - relevant cell contexts facilitates identification of candidate disease genes.

These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia); their goal is to generate 160 fully characterized, human DNA promoters of less than 4 kb (MiniPromoters) to drive gene expression in defined brain regions of therapeutic interest for studying disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic Lateral Sclerosis (Lou Gehrig's disease), Multiple Sclerosis, Spinocerebellar Ataxia, Depression, Autism, and Cancer.

,» Steven Zeisel from the University of North Carolina at Chapel Hill noted that choline and betaine work together in the cellular process of methylation, which is not only responsible for the removal of homocysteine, but is involved in turning off the promoter regions of genes involved in inflammation.

We identified a conserved sequence, CCCGCG, within this deletion by comparing the promoter regions of the dog, cow and horse Cox - 2 genes.

The present study suggests that this risk for depression is moderated by social supports and polymorphic variation of the VNTR in the promoter region of the serotonin transporter gene (5 - HTTLPR).

We studied the well known functional polymorphism in the 5 ′ flanking regulatory (promoter) region of the gene (SLC6A4) coding for the serotonin transporter protein.

However, researchers at the University of California — Berkeley wanted to find out if there might a genetic component that could predict how couple interactions affect marital satisfaction.5 Specifically, the team decided to look at the 5 - HTTLPR polymorphism in the promoter region of the serotonin transporter gene.

Interleukin - 10 gene promoter polymorphism in patients with schizophrenia in a region of East Turkey

This investigation examined the extent to which polymorphisms of the serotonin transporter linked promoter region (5 - HTTLPR) and the dopamine receptor D4 (DRD4) genes differentially influenced the development of attachment security and disorganization in maltreated and nonmaltreated infants at age 13 months, and the extent to which the efficacy of preventive interventions to promote attachment security were influenced by genetic variation.