Sentences with phrase «gene pyrosequencing»

Archaeal community composition in the sediments was analyzed with high - throughput 16S rRNA gene pyrosequencing.

One question in analyzing microbial communities by 16S rRNA gene pyrosequencing centers on whether the amplification and sequencing methods result in recovery of sequences in proportion to their representation in the original community [24].

Not exact matches

Microbial DNA from colon specimens were isolated and analyzed by use of 454 pyrosequencing of 16S RNA genes.

Here we use pyrosequencing of the bacterial 16S rRNA gene to quantify bacterial taxa [21].

Services include: consultation with investigators to chose the best method of analysis; sample preparation, assay design and processing of the most used methods to study DNA methylation, both gene - specific and genome - wide (PyroMeth, RRBS and Methyl - Seq); and assessment of global DNA methylation by pyrosequencing methylation analysis of repetitive elements (LINE - 1 and Alu repeats).

We investigated the distal gut bacterial communities of three healthy humans before and after treatment with ciprofloxacin, obtaining more than 7,000 full - length rRNA sequences and over 900,000 pyrosequencing reads from two hypervariable regions of the rRNA gene.

Computer simulations by Liu et al. [43], Wang et al. [41], and Sundquist et al. [47] have indicated that pyrosequencing tags from different regions of the gene will vary in their utility for the distinct tasks of revealing microbial diversity and performing taxonomic classification, both of which contribute to making informative comparisons between complex microbial communities.

Pyrosequencing of 16S rRNA genes and temporal temperature gradient electrophoresis (TTGE) profiles in the colonic digesta showed that rats fed RS had altered microbiota compositions due to blooms of Bacteroidetes and Actinobacteria.

Methodology / Principal Findings: Fecal samples from healthy dogs (n = 32), dogs with acute non-hemorrhagic diarrhea (NHD; n = 12), dogs with acute hemorrhagic diarrhea (AHD; n = 13), and dogs with active (n = 9) and therapeutically controlled idiopathic IBD (n = 10) were analyzed by 454 - pyrosequencing of the 16S rRNA gene and qPCR assays.

B. Correlation of the fold differences between CPA and control obtained by Q - MeDIP and by microarray for the 20 amplicons analyzed in A. C. DNA methylation differences (%) between the groups in the GRM5, ITGB6, OR13C8 and IL - 31 genes validated by pyrosequencing.