Sentences with phrase «gene rearrangement»

These assays, developed for use with specific Next Generation Sequencing (NGS) platforms, combine optimized, proprietary multiplex PCR master mixes with a software package that converts NGS output data to an easily interpretable form, enabling you to identify the DNA sequence, clonal prevalence, and V - J gene identity for each gene rearrangement.

Among the 119 B cell neoplasias, microscopic BM involvement was positive in 31 cases and Ig gene rearrangement was detected in 25 of 31.

As a reagent manufacturer, we offer a comprehensive selection of gel, capillary, and next - generation sequencing PCR assays for gene rearrangement, chromosome translocation, and gene mutation detection as analyte specific reagents (United States), research use only, or CE - marked in vitro diagnostics to laboratories (outside of North America).

In 6 cases, Ig / TCR gene rearrangement test did not detect BM involvement, which was presented by microscopy.

Duration of Symptoms Does Not Correlate with Results of T - cell Gene Rearrangement Studies in Patients Evaluated for Cutaneous T - Cell Lymphoma.

With our NGS - based assays, the gene rearrangement status of several million lymphocytes in blood, bone marrow, or tissue samples can be both identified and characterized.

Our bioinformatics team has expertly designed and validated software that can detect mutations or gene rearrangement sequences (clonotypes) at sensitivities as low as 10 - 4 (or even down to 10 - 6 if sufficient DNA input is available).

They also include a comprehensive bioinformatics package, enabling you to identify the DNA sequence, clonal prevalence, and V - J family identity for each gene rearrangement.

Among the 29 T cell neoplasias, microscopic BM involvement was positive in 6 cases and TCR gene rearrangement was detected in all of them.

• Testing revealed an ALK gene rearrangement when a sample of a patient's uterine leiomyosarcoma was profiled.

They then looked for DNA breaks associated with receptor gene rearrangement by using a version of the polymerase chain reaction that amplifies broken DNA.

In fact, somatic ETV6 translocation is the most common gene rearrangement in childhood leukemia.

The results suggest that, although the lack of light chain synthesis can be due to a lack of gene rearrangement, there may also be transcriptional regulation, which may also be important for the expression of light chain immunoglobulins in immature B cells.

In the fetal liver hybridomas, which were constructed from fetal liver cells and a tumor cell, no light chain gene rearrangement was observed, whereas in the 70Z / 3 cell line a kappa light chain rearrangement probably occurred.

About 4 percent of NSCLCs are driven by genetic aberrations called ALK gene rearrangements, according to Farace.

Multiplexed genetic screening for epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK) gene rearrangements and subsequent biomarker - guided treatment is cost - effective compared with standard chemotherapy treatment without any molecular testing in the metastatic non-small cell lung cancer (NSCLC) setting in the United States.

Dorothy Romanus, lead author of the study, states «this analysis supports the value of multiplexed testing for EGFR and ALK gene rearrangements followed by molecularly - guided therapy in decisions surrounding coverage of related testing and targeted therapy.

«Natural resistance to malaria linked to variation in human red blood cell receptors: First study to identify protective effect of glycophorin gene rearrangements on malaria.»

But the explanation also posed a puzzle: Those random gene rearrangements also produce T cells that could attack the body's own tissues.

MRD was assessed using polymerase chain reaction analysis of Ig / TCR gene rearrangements.

Since leukemias and lymphomas originate from the malignant transformation of individual lymphoid cells, all leukemias and lymphomas generally share one or more cell - specific or «clonal» antigen receptor gene rearrangements.

In 5 % (6/119) of B cell neoplasias, the BM involvements were detected by microscopic evaluation, but Ig gene rearrangements were negative.

The LymphoTrack ® Dx TRB Assay for the Illumina MiSeq ® is an in vitro diagnostic product intended for next - generation sequencing (NGS) based determination of the frequency distribution of TRB gene rearrangements in patients suspected with having lymphoproliferative disease.

Detection of immunoglobulin heavy chain gene rearrangements in classic hodgkin lymphoma using commercially available BIOMED - 2 primers.

Our NGS kits are used to detect clonal gene rearrangements, somatic hypermutations and for the study of MRD.

Several ongoing projects include the identification of newly discovered gene rearrangements in rare and refractory (unresponsive or resistant to therapy) tumors from patients evaluated at the Rutgers Cancer Institute of New Jersey.

To identify and track clonal T cell receptor (TRB) gene rearrangements using next - generation sequencing with the Illumina MiSeq ®

The laboratories also offer highly sensitive NGS - based MRD assays to track FLT3 mutations and patient - specific immunoglobulin and T - cell receptor gene rearrangements.

Several gene rearrangements have been engineered and their protein products have been expressed in both normal cells and cancer cells to understand the effects of each rearrangement on cell function and targeted drug effectiveness.

A number of Invivoscribe LymphoTrack Assays, formatted for use on both the Illumina MiSeq ® and Thermo Fisher Ion PGM ™ platforms, were designed to detect somatic gene rearrangements within the antigen receptor loci.

These comprehensive assays are capable of detecting single nucleotide substitutions, insertions, deletions and gene rearrangements.

Mitochondrial gene rearrangements confirm the parallel evolution of the crab - like form.

Not exact matches

To that end, the US National Center for Biotechnology Information in Bethesda is developing a database, ClinVar, to integrate clinical and genetic data; others, such as DECIPHER, run by the Sanger Institute, handle genetic data such as chromosome rearrangements that can disrupt genes.

«Rare genetic cause of peritoneal mesothelioma points to targeted therapy: Genetic rearrangement in the ALK gene found in young women with mesothelioma may be targetable with FDA - approved drugs.»

This study showed the two subtypes have different genetic origins and involve a dramatically different number of chromosomal rearrangements, mutations and other gene variations.

Now, through an unexpected observation and a meticulous study of patients seen at Brigham and Women's Hospital, BWH investigators have added a fourth cause to the list: a genetic rearrangement in the ALK gene, observed in three patients with peritoneal mesothelioma.

After enriching for CTCs, the researchers analyzed the samples for ALK rearrangements and for an increase in the number of copies of the ALK gene.

Two types of immature B cells, namely fetal liver hybridomas and the leukemic cell line 70Z / 3, both of which have cytoplasmic mu chains but no light chains, were examined for DNA rearrangements of their light chain and heavy chain immunoglobulin genes.

We found some people have a complex rearrangement of GYPA and GYPB genes, forming a hybrid glycophorin, and these people are less likely to develop severe complications of the disease.»

These differences include widespread sequence variation, extra copies of genes, and massive, complex rearrangements.

They discovered that people who have a particular rearrangement of the glycophorin genes had a 40 per cent reduced risk of severe malaria.

This caused the host bacteria to «spit» out the replicon from the genome, causing a rearrangement of the rapamycin biosynthetic genes.

The commercial tests were based on both Sanger sequencing and supplementary testing for large genomic rearrangements in both genes.

These rearrangements affected several known and suspected oncogenes and tumor suppressor genes, including Notch1, Pten, Ikzf1, Jak1, Phlda1, Trat1, and Agpat9.

«Other things also matter, such as gene mutations, rearrangements and changes in expression.

The growth of long facial feathers, creating the appearance of muffs and beards on chickens, is caused by a chromosomal rearrangement affecting a gene involved in feather development, report Xiaoxiang Hu of the China Agricultural University in Beijing and colleagues, in a new study published on June 2 in PLOS Genetics.

One likely explanation is that genes that have evolved separately, even without any chromosomal rearrangement, produce proteins incompatible with the proteins of other species, he says.

In another experiment, Kelsoe found that B cells expressing the rearrangement genes bound less tightly to an antigen than did B cells that didn't rearrange their DNA.

How these self - promoting genes jump to these prime locations remains a mystery, but the researchers believe it may have to do with the DNA's three - dimensional structure in particular areas that allows for genetic rearrangement when the genome is being copied or when certain genes are active.

Tipped off by a suspicious chromosomal rearrangement, researchers have identified a gene they believe causes Tourette syndrome when mutated.