Sentences with phrase «gene sequencing labs»

NIST's reference DNA will enable gene sequencing labs to compare and calibrate their results to the same standard.

Not exact matches

An international team led by researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) has developed a new technique for identifying gene enhancers — sequences of DNA that act to amplify the expression of a specific gene — in the genomes of humans and other mammals.

Rudenko and Oliver send DNA from the cultured spirochetes for gene sequencing at a lab at the University of Washington in Seattle.

I had zero computer experience; in fact, I could barely type, but a postdoc in the lab helped me do a BLAST search using my gene's protein sequence as a query.

These are sequences made in the lab from RNA — the template used to produce the proteins that genes code for.

The gene - editing success appears to be largely due to one procedural change: The researchers introduced the editing system — the enzyme Cas9 and a guide RNA sequence that helps the editing machinery find its target — at the same time they injected the mutation - laden sperm into a healthy egg in the lab.

At Darwin's lab, eight sequencing machines ran around the clock, decoding the DNA samples that had been collected from Ginger and the others, while Schellenberg and George Martin and their colleagues combed through the results for suspect genes.

Conlon's team is now sequencing P. aeruginosa strains to see how gene sequences vary between strains and how this variance affects the ability of these strains to produce the aforementioned factors Conlon's lab has described.

Both studies relied on a popular lab organism known as C. elegans, a nearly microscopic nematode that is fast growing, translucent and has a sequenced genome showing that nearly half its genes are closely related to corresponding human genes.

To begin to grapple with those questions, the researchers involved in the new study first selected as «seeds» the nine genes that have been most strongly tied to ASDs in recent sequencing research from their labs and others.

To narrow down the suspects in the US Airways crash, the Smithsonian lab's Carla Dove isolated and sequenced a small piece of a mitochondrial gene — known in the field as a DNA bar code.

These are sequences produced in the lab from the RNA that is the intermediate step between gene and the protein it codes for.

Using single cell RNA sequencing analysis, the Cairns lab profiled cells individually, establishing the gene expression profile in human sperm stem cells.

Back in the lab in Norman, Lewis and his colleagues used state - of - the - art gene sequencing methods that allowed them to get long segments of the gene that is used as the standard for classification and identification of microbes, because it differs in various bacteria.

«Understanding how Cas9 is able to locate specific 20 - base - pair target sequences within genomes that are millions to billions of base pairs long may enable improvements to gene targeting and genome editing efforts in bacteria and other types of cells,» says Doudna who holds joint appointments with Berkeley Lab's Physical Biosciences Division and UC Berkeley's Department of Molecular and Cell Biology and Department of Chemistry, and is also an investigator with the Howard Hughes Medical Institute (HHMI).

Collaborating with the labs of Salk Professors Joseph Ecker and Alan Saghatelian, the Izpisua Belmonte team performed extensive characterization of the new cells and found rsPSCs showed distinct molecular and metabolic characteristics as well as novel epigenetic signatures — that is, patterns of chemical modifications to DNA that control which genes are turned on or off without changing the DNA sequence.

This involves «knocking out» a gene sequence from the mouse genome and inserting an artificial gene sequence that has been generated in the lab.

Sequencing the diploid ancestors of wheat, some of which was done in the McCombie lab, enabled the team to computationally dissect out which sequences were gene copies and which were repeats.

By comparing the whole genome sequence of the lab - cultured resistant strain with a sibling strain that had not been exposed to the drug, they were able to identify several mutations of interest in a number of genes.

«There could be labs out there who have already sequenced a similar gene and the protein associated with it and worked on its function, but haven't published yet,» he said.

Berkeley Lab's Len Pennacchio (left) and Axel Visel led the development of a new technique for identifying gene enhancers called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing.

Zoghbi's lab also found that this altered ATXN1 gene sequence encodes a bulky, improperly folded protein that is toxic to brain cells.

Thanks to innovations in sequencing technology that have produced a cornucopia of genomes, plus some tweaks to the computational methods by different labs, the combined list of identified HARs now includes nearly 3,000 genome segments.4 But the original trend still holds; nearly all HARs are outside genes, some quite far away from any gene in the genome.

Zoghbi's lab meticulously analyzed each gene in this region, systematically eliminating genes based on sequence differences between healthy controls and Rett syndrome patients.

«Before there was the Human Genome Project, people were studying gene sequences — they were just doing it a million different ways in a million different labs,» Huerta says.

In particular, the lab investigates how alterations in epigenetics — or the heritable changes that affects gene expression without alterations in the underlying DNA sequence — contribute to epithelial ovarian cancer.

Ryan Morin, a graduate student in the Marra lab, walked us through a summary of transcriptome sequencing and how it can be used not only for gene expression, but also discovery of mutations and characterization of alternate splicing.

Laboratory activities minimally include performing a lab - based patient - oriented research project applicable to cancer genomics, with an emphasis on mastering techniques commonly used within a human genetics laboratory, such as PCR, gene sequencing, mutation analysis, western blots, and functional protein studies.

«In the old days, two or three years ago, we would find an interesting Arabidopsis mutant in the lab, and then there was the year - long haul to find the gene responsible for those physical characteristics,» says Steve Rounsley, formerly of TIGR, where Arabidopsis chromosome 2 was sequenced.

To that end, Berkeley Lab researchers will use CRISPR / Cas9 gene - editing technology, which Berkeley Lab scientist Jennifer Doudna helped pioneer, to systematically test the function of representative sequences in mice.

With the completion and publication of multiple fully sequenced Woolly Mammoth genomes, the Church lab is discovering potential cold climate adaptations in different genes.

Grace, in turn, does her part, interning at the lab in the gene sequencing department daring to believe that one day they might make a breakthrough... and one day they do.

Tags for this Online Resume: CL (Command Language for AS / 400), High Performance Liquid Chromatography (HPLC), Research, microbiology, HPLC, GC, IC, Bioinformatics, DNA / RNA extraction, Next - generation sequencing, Cultivation, Wet lab work, Molecular biology, Gene cloning, Biogeochemistry, Oceanography, Marine biology, Field work, Research cruise