More sensitive cultivation methods and precise 16S rRNA
gene sequencing techniques have revealed that the human bladder hosts a significant microbiome and those diverse bacteria inside the bladder impact pediatric urologic diseases.
New, sophisticated
gene sequencing techniques are leading to an increasing understanding of the causes of genetic disease, and can help parents with affected children make informed reproductive choices, the annual conference of the European Society of Human Genetics will hear.
In - depth analysis of the human body's microflora has been possible only in the past few years — a by - product of the same new
gene sequencing techniques that have allowed scientists to cheaply and accurately identify the DNA of the human genome.
Not exact matches
The CRISPR approach is very good at disabling
genes, but using the
technique to fix them is much harder, because it involves replacing a faulty
sequence with another.
Using a
gene -
sequencing technique known as whole - exome
sequencing that targets only the DNA that encodes proteins, the researchers cataloged virtually every significant DNA variant in three families in which the condition is common.
These are subjected to so - called next generation
sequencing, a
technique that sifts the vast genetic composite, ferreting out
sequence variants (or alleles) caused by mutations in
genes.
An international team led by researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) has developed a new
technique for identifying
gene enhancers —
sequences of DNA that act to amplify the expression of a specific
gene — in the genomes of humans and other mammals.
Thanks to powerful
gene -
sequencing techniques developed in the past two decades during the race to decode the human genome, researchers are beginning to reconstruct what our ancestors» microbiomes looked like, potentially going back thousands of years.
Using a
technique known as single - cell RNA
sequencing, the team explored more than 65,000 individual cells that exist under normal or inflammatory conditions, looking for
genes that were more active in one state or subpopulation versus another.
The researchers then compared the expression of all
genes in six parts of the brain of the two bird species using state - of - the - art molecular
techniques, including next - generation
sequencing — the first time these tools have been used to find brain properties related to innovation and problem - solving in wild birds.
So to reveal their function, biologists used the
sequence information along with a
technique called RNA - mediated interference (RNAi) to temporarily inactivate each of nearly 17,000 of the worm's
genes.
The
technique involves designing double - stranded RNA molecules that match a
sequence in the RNA produced by the
gene you want to inactivate.
As when he worked on the human genome, Venter is relying on a radical
technique called shotgun
sequencing: He chops up vast amounts of DNA into tiny pieces and then uses sophisticated computer analyzers to piece them back together into intelligible
genes and chromosomes.
These tags are detected and measured using a sensitive DNA analysis
technique known as massive, parallel
sequencing, providing a quantitative readout of the
gene's activity levels.
They identified a total of 164 barnacle larvae under a dissection microscope, extracted DNA from the larvae, and used standard
techniques to
sequence mitochondrial COI
genes, often called a barcode
sequence.
They used a
technique called next generation
sequencing (NGS) to detect specific bacterial
genes.
Like Wray, the scientists estimated the
genes» rate of evolution by comparing their DNA
sequences in two different species, a
technique calibrated to the date when the two species are known to have diverged.
«Previous
techniques that have been used to investigate DNA control
sequences usually rely on sorting cells one by one and measuring
gene activity in each of them,» says Dr Eva Yus, lead author of the paper.
The genome - editing
technique known as CRISPR allows scientists to clip a specific DNA
sequence and replace it with a new one, offering the potential to cure diseases caused by defective
genes.
Michael Metzker, at the time a graduate student in the laboratory of Richard Gibbs at Baylor College of Medicine in Houston, compared the
gene sequences of the strains to see how closely related they were, using a
technique called phylogenetic analysis.
According to Richard Durbin, who leads the project's informatics team, computer
techniques seem to find most of the
genes sequenced so far but frequently missed some pieces of them, particularly the short coding regions that occur at the beginning of many nematode
genes.
Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger -
sequencing techniques to ensure the accuracy and knockout of the target
gene.
The advent of genomics and rapid
sequencing techniques has seen HeLa cells used in numerous large - scale studies of
gene function and expression.
Bioinformatic approaches to the analysis of genetic variability and complex genotype - phenotype relationships will moreover include
gene sequence and database analyses, measures of association of haplotypes / genotypes with phenotype, clustering procedures, neuronal networks, fuzzy and other
techniques in pattern recognition, similarity measures for discrete patterns (e.g.,
gene sequences, structures, functions), logistic regression methods, and a spectrum of other
techniques.
The team used a new approach to
gene sequencing that enabled them to completely reconstruct the virus» previously unknown genome in less than 24 hours — significantly faster than conventional
sequencing techniques, which can take days to weeks.
A new
technique for
sequencing the RNA in a single cell has been revolutionary, Ngai said, allowing researchers to trace which stem cells in a densely packed tissue become specialized, based on the mRNA present in the cell, which indicates which
genes are being expressed.
Using a
technique called whole - genome random
sequencing, a TIGR team mapped the entire two - million -
gene sequence of Thermotoga maritima, a bacterium first plucked from hot geothermal springs in Vulcano, Italy.
The human genome contains about 3 billion base pairs, but only about 2 percent of these base pairs represent protein - coding
genes, meaning that whole - exome
sequencing measures the genetic alterations focused on a small but very important fraction of the genome (as opposed to
techniques of whole genome
sequencing, which measures every nucleotide across the entire genome, regardless of whether these
genes are expressed or silent).
This complex and computer - heavy
technique looks at the bacterium's entire DNA, locating its
genes along the
sequence.
The researchers collected liver samples, and looked at
genes in the liver affected by e-cigarettes using a
technique called RNA
sequence analysis.
Traditional genetic methods, such as looking at family trees and
sequencing individual
genes, have linked defects in 80
genes on the X chromosome to XLMR, but the discovery rate has slowed, suggesting that those
techniques have reached their limit.
The Yale - led study performed whole exome
sequencing ¬ — a
technique for
sequencing all the expressed
genes in a genome ¬ — on TNBC tumors to identify mutations in specific
genes or pathways that may indicate response or resistance to the standard of care, which is anthracycline / taxane (ACT) chemotherapy.
Their discovery had to wait for the
sequencing of the human genome and other advances in
gene - finding
techniques.
To test their theory, the researchers investigated what would happen to fetal mouse brains if they interfered with Trnp1 expression using synthetic
sequences of genetic material that silenced the
gene, a
technique called RNA interference.
She and other scientists are trying various
techniques to streamline the
gene analysis process, such as discarding less common
gene sequences in a particular sample in order to focus on more prevalent ones.
It currently analyses a panel of multiple cancer predisposition
genes thanks to new - generation
sequencing techniques.
Next, they extracted the DNA from developing and mature heart cells, using an advanced
gene -
sequencing technique called ChIP - seq that lets scientists «see» the epigenetic signatures written in the DNA.
Using an array of
techniques, including RNA
sequencing and targeted
gene expression profiling, the researchers searched for links between
gene activity and protein production in these tumors and the clinical outcomes of patients.
And doctors at the Great Ormond Street Hospital in London recently reported using a similar
gene - editing
technique called TALENs, which also recognizes and cuts precise DNA
sequences, to engineer immune cells for a therapy that may have cured two infants of leukemia.
Berkeley Lab's Len Pennacchio (left) and Axel Visel led the development of a new
technique for identifying
gene enhancers called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by
sequencing.
To find a solution to this, Marcel Martin at Bioinformatics Long - term Support (WABI) at SciLifeLab, in collaboration with the group of Gunilla Karlsson Hedestam at Karolinska Institutet, has developed a practical, computational
technique, IgDiscover, that enables rapid production of individualized V
gene databases following deep
sequencing of expressed antibody repertoires.
has developed a practical, computational
technique, IgDiscover, that enables rapid production of individualized V
gene databases following deep
sequencing of expressed antibody repertoires.
We are using a new
technique, called single cell RNA
sequencing, to isolate thousands of single neurons from human brain tissue, study all the
genes that are expressed in each individual cell, and make cell - to - cell comparisons between normal, early stage and late stage AD.
This open - ended approach relies heavily on RNA
sequencing, a cutting - edge
technique to identify
genes whose expression is enhanced or diminished by a particular condition.
Robert Darnell uses high throughput
sequencing and other
techniques to investigate the proteins that regulate RNA and thereby affect
gene expression.
Home > Press > Single - cell mRNA cytometry via
sequence - specific nanoparticle clustering and trapping: Cell - to - cell variation in
gene expression creates a need for
techniques that can characterize expression at the level of individual cells
Laboratory activities minimally include performing a lab - based patient - oriented research project applicable to cancer genomics, with an emphasis on mastering
techniques commonly used within a human genetics laboratory, such as PCR,
gene sequencing, mutation analysis, western blots, and functional protein studies.
Essentially, the process uses genetic
techniques to target
sequences of DNA inside the mutant
gene.