Sentences with phrase «gene silencing using»

In the Baulcombe lab, Voinnet analyzed the in vivo dynamics of gene silencing using transgenic plants, recombinant viruses, and fluorescence microscopy.

Not exact matches

BOSTON, April 20 (Reuters)- Alnylam Pharmaceuticals Inc said on Friday that Dicerna Pharmaceuticals Inc will pay it $ 25 million in cash and stock to resolve a lawsuit claiming it stole trade secrets about gene - silencing technology used to develop drug treatments.

To confirm this observation, the researchers used siRNAs (molecules that silence gene expression) to turn off the RAC1 gene, which synthesizes Rac1 protein.

«These particles, microRNA based SNAs, could also potentially be used for gene silencing in other cancers and diseases of genetic origin.»

Using the new data, Laura Carrel, a geneticist at Pennsylvania State College of Medicine in Hershey, and Huntington Willard, a geneticist at Duke University in Durham, North Carolina, discovered that 15 percent of the genes on the inactivated X chromosome are actually not silenced — and most are likely to be expressed at higher levels overall in women than men.

«We can use different approaches to reverse methylation,» she says, «turning silenced genes back on, and seeing whether we can prevent the tumours from occurring or treat the cancer after it has appeared.»

The cut DNA is then either left as - is, silencing it, or repaired by using the gene editor to slip in a new, functioning segment.

Study shows memories formed by the same gene - silencing tool used in embryonic development; a finding could set the stage for new therapies for schizophrenia

The gene is silenced because it is on the part of their chromosome they inherited from their mother, and for mysterious reasons our cells use the father's copy of this gene.

Not only is RNAi used to regulate gene expression within an organism, it also can combat viral infection by silencing the activity of viral genes required for the pathogen's replication.

Indeed using genome - wide transcriptional profiling, the researchers revealed that expression of clipped H3.3 silences genes that regulate the division and duplication of a cell.

«So, with this knowledge, the dream is that we could eventually use gene editing technology to edit the microRNA target sites in the host plants, preventing the microRNAs from binding and silencing these genes.

His current specific interest is the elucidation of novel mechanisms of drug resistance through the discovery / identification of novel gene function (s) and phenotypes using RNAi - mediated gene silencing or cDNA overexpression.

An integrative approach to data generation includes strict validation criteria using gene silencing, paired antibodies, and fluorescently tagged proteins.

It has billions of parts, many used to activate and silence genes — an arrangement that allows individual cells to specialize as brain cells, heart cells and some 200 other different cell types.

RNAi uses bits of RNA — the chemical cousin of DNA — to silence specific genes.

Foltz's team has also used the mouse atlas to help home in on two genes, known as BEX1 and BEX2, which seem to be silenced in a form of brain cancer called glioma.

On its own, gene silencing is also being used to create GM crops.

But so far, silencing genes using RNAi has not worked well in mammalian cells; although the approach has been successful in mouse embryos, double - stranded RNA shuts down synthesis of all proteins, not just the target gene, in other types of mammalian cells.

RNAi silences genes using small pieces of RNA called small interfering RNAs (siRNAs).

His group uses genome - wide and gene - specific DNA methylation analysis to identify aberrantly silenced tumor suppressor genes in B cell leukemias and lymphomas.

To test their theory, the researchers investigated what would happen to fetal mouse brains if they interfered with Trnp1 expression using synthetic sequences of genetic material that silenced the gene, a technique called RNA interference.

When the birds were 23 days old, the age at which song learning is known to begin, the researchers injected the gene - silencing RNA strands into seven male zebra finches» brains, using 10 finches as controls.

First author Dr Reza Haqshenas said the researchers then used gene silencing technology to determine whether the genes» cell factors identified using the antibody microarray were indeed important for HCV replication and therefore potential targets for anti-HCV compounds.

They also used plants that were inhibited in their ability to produce floral volatiles after the biosynthetic gene NaChal1 (CHAL) had been silenced.

A type of gene silencing treatment in which specially designed DNA molecules are used to switch off a gene

A way of silencing genes using specially designed molecules of RNA — like DNA but made of only a single strand — that target the message molecules in cells and tell them not to make a certain protein

Gene silencing is a natural adaptive defense mechanism used by plants against viruses.

A type of gene silencing treatment in which specially designed RNA molecules are used to switch off a gene

The third PDR approach and the one that we intend using not only for MSV resistance but also for other viruses such as MRDV, is based on something called RNA - mediated gene silencing.

Unpaired student's t - test was also used to compare gene expression variation in siTRF2 silenced versus non silenced scrambled siRNA HCT116 cells in the presence of bleomycin sulfate.

By the early 2000s, Joshua - Tor and colleagues including Rob Martienssen and Greg Hannon were using biochemical and structural methods to lay bare the mechanism that enabled RNAi to silence genes in a highly specific manner.

We also show that inhibition of mTOR with rapamycin, as well as silencing mTOR gene product expression using mTOR - specific small interfering RNA, decreased phosphorylation of mTOR signaling proteins and induced cell cycle arrest and apoptosis in ALK + ALCL cells.

They discovered that CRISPRi was much more efficient than CRISPR - Cas9: in more than 95 % of the cells created using CRISPRi, the target gene was silenced, compared with only 60 - 70 % of cells grown from CRISPR - Cas9.

We confirmed the role of Nrf2 in the regulation of HMOX1 gene expression by HPP - 4382 using genetic silencing of Nrf2.

«Now that we have the gene, we can actually use that gene sequence to temporarily silence the gene and make rye and other chromosomes pair with wheat and transfer genes by a natural method into wheat without calling it GMO,» Gill said.

Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments.

One such evolutionary signal is RNA interference, a process that cells use to turn down or silence the activity of specific genes.

Cleveland and the members of his lab use these therapies, also known as designer DNA drugs, to silence genes involved in the development of various neurodegenerative diseases such as Alzheimer's, Huntington's and Lou Gehrig's disease.

The research, published in the journal Nature, described the use of RNA interference (RNAi) technology to silence genes involved in the metabolism of cholesterol read more

Van Meir cautions: «We need to have a better understanding of all the genes that are turned on or off by silencing MBD2 in a given cancer before we can envision to use this approach for therapy.»

Scientists have identified a protein the Piwi system uses to guide a cell's gene - silencing machinery to the right spots in the genome...

1) Plant - mediated gene silencing: Using plant - mediated gene silencing, scientists induce plants to transfer pieces of genetic material to other organisms, targeting and interfering with the interactions between plants and their pests at the genetic level.