Sentences with phrase «gene targeting vectors»

In that year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA.

from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA

Not exact matches

I won't reveal yet who my favorites are, but I will say that these young scientist - founders came up with very creative solutions for preventing infections in some common surgeries, tackling resistance in targeted antibody drugs, improving gene vectors for cell therapies, helping the vision - impaired «see» faces and better read their environments, imaging hard - to - see spots in the lungs and other organs, improving genetic risk analysis, and expediting the logistical operations of hospitals.

The vector then unloads its genetic material containing the therapeutic human gene into the target cell.

A carrier called a vector must be used to deliver the therapeutic gene to the patient's target cells.

The aim is to introduce into the shuttle a viral vector loaded with the gene that the cells of these patients lack, with a particular focus on targeting neurons.

Still, the standard form of liver - targeted gene therapy carries a range of potential complications, including the risk of harmful mutations and of the body mounting an immune response against the viral vectors used to carry the correct forms of the defective genes responsible for haemophilia.

As the central nervous system is also a target of AAV vectors, Hélène Puccio and Patrick Aubourg's teams are investigating whether a similar approach using gene therapy could be as effective for the spinal cord and cerebellum as it is for the heart.

The non-pathogenic vectors serve as gene shuttles to transport components of SIV, such as envelope proteins, into the target cells.

«Human gene targeting by viral vectors.»

The ZFNs targeting either the cxcr4 or ccr5 genes were linked via a 2A peptide sequence and cloned into the pAdEasy - 1 / F35 vector under control of the CMV TetO promoter, and the Ad5 / F35 virus for each construct was generated using TREx 293T cells as described [33].

Vaxwave ® is based on lymphocytic choriomeningitis virus (LCMV) and in this vector the gene encoding the LCMV envelope protein, normally responsible for virus entry into target cells, has been deleted and replaced with a target gene of interest.

A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA.

My laboratory has already developed molecules and gene transfer vectors that can target either the MAC, through the delivery of a protein called CD59, or the anaphylatoxins by delivery of proteases, or both by a protein called PRELP.

Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129 - derived ES cell lines.

The gene - targeting approach developed by Suzuki and his colleagues relies on the use of so - called helper - dependent adenoviral vector to deliver large mutation - free DNA molecules into cells.

A targeting vector was designed to contain the Enhanced Yellow Fluorescent Protein gene (from the pEYFP - N1 plasmid, Clonetech) downstream of a loxP - flanked stop sequence (neomycin resistance gene and a trimer of the SV40 polyadenylation sequence).

Students can perform RNAi «from scratch» using bioinformatics to develop PCR primers for a target gene, then cloning the amplified product into an RNAi feeding vector, and finally observing the phenotype of treated worms.

A featured paper in the February issue of the research journal Cancer Gene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differences between cancerous and normal liver ceGene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differences between cancerous and normal liver cegene therapy using adenoviral vectors, based upon a fundamental new understanding of the differences between cancerous and normal liver cells.

Their contributions to the yeast community include physical mapping methods, synthetic lethality screen approaches for identifying cross-species candidate genes as potential cancer drug targets, and a widely used set of vectors and yeast host strains that have been instrumental in work that has led to countless discoveries in recent decades.

2/13/2007 Study Shows Liver an Excellent Target For Cancer Gene Therapy Using Viral Vectors A featured paper in the February issue of the research journal Cancer Gene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differen... MorGene Therapy Using Viral Vectors A featured paper in the February issue of the research journal Cancer Gene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differen... Vectors A featured paper in the February issue of the research journal Cancer Gene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differen... MorGene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differen... Morgene therapy using adenoviral vectors, based upon a fundamental new understanding of the differen... vectors, based upon a fundamental new understanding of the differen... More...

A robot like this could potentially be used to infuse drugs at targeted points in the brain, or to deliver gene therapy vectors.

Gene Therapy for Cardiovascular Disease: Advances in Vector Development, Targeting, and Delivery for Clinical Translation.

It is always possible that a vector will introduce the gene into a cell other than that for which it is supposed to be targeted (e.g., a spermatocytic cell) or that through a secondary mechanism target cells that have taken up the new gene will through some independent natural process (such as transfection) transfer the gene to a germline cell.

Future use of IGF - 1 will no doubt involve gene therapy, which directly targets genes that produce IGF - 1 in muscle, usually by attaching specific gene activators to an inactive virus or vector that then enters into muscle cells.

A vector, which may be a virus, a vaccine, or a molecule such as a liposome (used to encapsulate and protect the gene until it reaches the target tissue), is required to deliver the gene to the target tissue.