Sentences with phrase «gene targets»

However, scientists are still barely scratching the surface of the full capabilities of gene targeting technology.

No one had demonstrated gene targeting in animal cells.

• ES cell line purity verification In addition to mouse strain purity verification, the facility provides genetic screening of ES cell lines used for gene targeting experiments.

We used gene targeting and computational modeling to test this new hypothesis.

In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome.

Also, because of the need for drug selection, the targeting construct in old - school gene targeting has to be a blunt instrument.

Career Goals: My goal is to pursue a career in scientific research that focuses on identifying gene targets for treating / ameliorating immune - mediated inflammatory diseases.

Although CRISPR is incredibly useful for generating mutations by NHEJ and generating small mutations with HDR, when it comes to larger scale genome editing, such as replacement of a mouse gene with its human ortholog (greater than 5 kb), it remains to be seen whether CRISPR is as robust as conventional gene targeting.

In order to identify the relationship between burst firing and absence seizures, the researchers conducted an experiment to induce absence seizures in mice using gene targeting techniques to delete the T - type calcium channel CaV3.3.

Their research has created the technology known as gene targeting, now used in virtually all areas of biomedicine — from basic research to the development of new therapies.

Design and generate gene targeting constructs, including knockin report genes, knockin mutations, conventional / conditional knockouts, and BAC transgenics and constructs for CRISPR / cas9 targeting

Since proper functioning of the fat body is essential for the development of the female reproductive system after a blood meal, identifying which miRNAs are important to fat body functions, and what specific genes they target, can help design ways to manipulate the levels of microRNA or their targets, affect their interactions, disrupt mosquito reproduction, and thus prevent the spread of diseases the mosquitoes transmit.

The CRISPR / Cas technology applied to mouse genetic engineering could quickly advance scientific understanding of disease mechanisms by allowing researchers to ask complex questions and find answers much faster than with traditional gene targeting approaches.

The zebrafish facility at SciLifeLab has participated in the development of a high - throughput gene targeting method in zebrafish, presented in the July issue of Genome Research.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

The resultant gene targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1 - 40 % after G418 selection (Kohli et al 2004, Kim et al 2008).

The resultant gene targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1 - 40 % after G418 selection -LRB-

from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA

«Human gene targeting by viral vectors.»

Cas9 / gRNA - mediated gene targeting demonstrated in this study can be used for other livestock species, which will contribute to advancing transgenic applications of large animal in biomedical and agricultural sciences.

Tamara Caspary, a developmental biologist and scientific director of Emoryâ $ ™ s transgenic mouse and gene targeting core, says she and her core team are in the process of developing and validating CRISPR, so that the technique could be accessible to many Emory investigators.

We plan to develop new methods and tools for (1) improving the prediction of TFBSs; (2) predicting TF gene targets; (3) prioritizing cis - regulatory variants dysregulating microRNA transcriptional regulation with downstream impact on the gene regulatory programs in cancer.

If Rudenko confirms Clark's PCR tests using additional gene targets, and if Clark or Rudenko manage to grow Borrelia cultures out of human and lone star tick samples, then even the fiercest skeptics will have to recognize that Southern Lyme strains threaten human health, and that Lyme - like illness deserves Lyme - like treatment.

The project will identify a potential translational approach for promoting optic nerve regeneration, and also open a new avenue for identifying novel gene targets that can be manipulated to enhance optic nerve regeneration.

Dr. Radislav Sedlacek and his team from the Institute of Molecular Genetics has recently published new paper on gene targeting of Rosa26 locus using TALENs.

In the current study, the LDA approach allowed simultaneous analysis of multiple gene targets, indicating more global transcription activity of persisting non-cultivable B. burgdorferi at 12 months.

Gene targeting makes it possible to study the effects of single genes, including those that don't occur naturally in mice.

However, it is costly and time - consuming to produce genetically modified livestock animals using standard homologous recombination gene targeting.

This led him to undertake a series of studies beginning in 1977 that demonstrated gene targeting in animal cells and culminated in the construction of one of the first knockout mice in 1989.

Carlo Croce, a cancer researcher at Ohio State University in Columbus, and his colleagues created a diagram of interacting miRNAs for normal body cells by connecting them according to which genes they target and the function of those genes, in a way similar to analyses of human social networks.

Gene Targeting When we create egg and sperm on demand, we may not have to pass along our complement of genes as is.

«Microsatellite length and location appears to be essential for EWS / FLI to activate the oncogenic gene targets,» says Dr. Lessnick.

«The ability to identify, characterize and validate gene targets that strongly associate or correlate with disease development or metastasis will facilitate early detection and appropriate treatments to tackle the disease at an early stage or before metastasis occurs.»

As an investigator in TGen's Pharmaceutical Genomics Division, Dr. Azorsa applied siRNA screening to identify novel therapeutic targets in cancer as well as functionally relevant gene targets that modulate drug response.

Dr George Vassiliou, joint project leader from the Sanger Institute and Consultant Haematologist at Cambridge University Hospitals NHS Trust, said: «This research has led to the identification of many potential gene targets for future AML therapy, which we are making available to other researchers to explore.

Other investigators who had been working to develop high efficiency gene targeting approaches improved on this finding with the addition of selectable markers that are only expressed when the targeting vector is inserted downstream of a functional promoter (gene traps).

CRISPR / Cas9 is a full gene editing tool designed to produce a genetically modified cell using any mammalian cell line and targeting any gene

Currently, such gene targeting is not possible in zebrafish or any vertebrate animal model system other than mice, for which such targeting involves the use of embryonic stem cells.

Results of the evaluation of applications for Microinjectionist — Transgenics & Gene Targeting Facility (call of interest of 10/02/2014).

An area of long - standing and exceptional strength of the Cancer Center is basic science discovery into novel cancer gene targets and their mechanisms.

Embryonic endothelial progenitor cells armed with a suicide gene target hypoxic lung metastases after intravenous delivery.

And CRISPR has completely and utterly transformed the field, as we can do rapid gene targeting now in primary human neural stem cells.»

All three isolates were characterized as Gram negative rods and are PCR positive for B. pseudomallei (lpxO gene target).

Zhang engineered light - sensitive proteins called channelrhodopsin and halorhodopsin into the neurons of freely moving mice using a virus - based gene targeting and delivery system.