Sentences with phrase «generated by gene»

CCHa2 - RKO51 - 2 and CCHa2 - RTAL - 34 are putative null alleles, which were generated by gene targeting using homologous recombination and TALEN, respectively (see below).

CCHa2 - RKO51 - 2 was generated by gene targeting using homologous recombination [22][23].

(A) The CCHa2 - RKO51 - 2 allele was generated by gene targeting using homologous recombination.

To investigate the role of cleavage in vivo, we generated by gene targeting a Pkd1 knockin mouse (Pkd1 (V / V)-RRB- that expresses noncleavable PC1.

Promoting survival and proliferation at the single - cell level is critical for expansion of any clonal colonies containing the mutation of interest generated by the gene editing process.

Homozygous mutant mice in which the Jak3 gene had been disrupted were generated by gene targeting.

We can compare the diverse tasks performed by a colony to the many proteins generated by gene transcription, to various cell types of a developing embryo, or to the firing patterns of neurons in the brain.

Not exact matches

While the entity generated by deleting or disabling early embryonic genes would produce only an unorganized collection of stem cells, it would do so after a period of what appears to be relatively normal development.

The issue should be understood as «which is more important, the individual replicator (the gene), or the patterns generated by interactions of a myriad of replicators with a complex environment?»

The shared patterns of gene expression in the limbs and phallus are generated in part by a common set of noncoding DNA, also called «elements» or «enhancers,» which act to control gene expression in both of these structures, argues a study published October 1 in Developmental Cell.

To generate the models, Jackson created two mutated versions of the PrP - coding gene by changing a single codon — one of the three - nucleotide «words» in genes that code for the various amino acids in proteins.

Goats as Drug Factories Initially, GTC generated transgenic goats by microinjecting into the developing nucleus of a one - cell embryo a gene encoding the desired human protein (along with DNA that promotes activation of that gene in milk).

Once such a gene is generated, bacteria can then transfer the genetic information in a horizontal fashion (between individuals) by plasmid exchange.

«By using chloroplast transformation we generated potato plants that accumulate high amounts of long stable dsRNAs targeting essential genes in the beetle,» says Ralph Bock.

The disease is caused by a mutation to the Werner syndrome RecQ helicase - like gene, known as the WRN gene for short, which generates the WRN protein.

Using Epiviz and Bioconductor, the research team found consistent regions of DNA methylation changes in colon cancer samples generated by the public Cancer Genome Atlas project and similar gene expression in these regions of DNA methylation changes in other cancer types.

This was found to be due in part to enrichment of genes involved in sugar metabolism and transport, likely an adaptation to photosynthesis - derived plant carbon, generated by nature's «candy factories,» said Asaf Levy.

To confirm these observations, both studies disabled the ap2 - g gene by cutting it out of the genome to remove its function from the parasite and the manipulated parasites indeed lost the ability to generate sexual - stage parasites.

Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.

Mice generated from embryonic stem cells in which ion channel genes have been mutated by homologous recombination often have a perfectly normal heart.

The audience will learn about the current state of breast cancer research, how data generated by NGS gene panels target variants of interest and have been developed and used in routine laboratory research, and the broader issues of breast cancer education, awareness, and community services.

Light signals from the eyes keep the internal circadian rhythm generated by clock genes coordinated with the environment.

«It is possible that the formation of the loop needed to make contact with the Sox2 gene is an important final step in the process by which researchers practicing regenerative medicine can generate pluripotent cells from adult cells.»

The discovery generated worldwide headlines, but some teams were unable to replicate the findings and the actual genes have not been found — not even by a team that vindicated Hamer's identification of Xq28 in a sample size 10 times larger than his last year.

The proof that STAG3 mutation is the cause of the disease has been achieved by generating mutant mice of this gene.

Yilmaz, who studies colon cancer and how it is influenced by genes, diet, and aging, decided to adapt this approach to generate colon tumors in mice.

The main change, a point mutation in the human gene for hu14.18, was designed to address treatment - limiting pain by generating a more tailored response that avoided triggering part of the immune response called the complement cascade.

The sweet receptor is actually made up of two coupled proteins generated by two separate genes: known as Tas1r2 and Tas1r3.

Then they generated larvae with a suppressed glycosidase activity by silencing the respective larval glycosidase gene to study the effect of lyciumoside IV ingestion on the disabled larvae.

«By combining an interrogation of gene networks with advanced data analysis and statistics, we have generated clinically meaningful information about stress and aging that is relevant to the broader population.»

We have tested this hypothesis by generating and analyzing a mouse strain that lacks the gene encoding the essential subunit of the N - methyl - d - aspartate (NMDA) receptor NR1, specifically in dentate gyrus granule cells.

The RPE cells generated by Takahashi's team show the characteristic structure and gene expression pattern of authentic human RPE cells.

As well as generating mutations by inserting themselves into and disrupting genes, retrotransposons can alter gene activity if inserted into adjacent regulatory regions of DNA.

Deep sequencing analysis has shown that this is highly unlikely in the case of J3 as no sequences were generated by deep sequencing of the seven naïve llamas or three other immunized llamas that share greater identity with J3 than its putative germ line V gene.

ReNeuron can generate large numbers of stable cell lines by engineering cells with a modified version of the gene c - myc.

The transcriptomics and phosphoproteomics data generated by this project will allow the identification of novel host and parasite genes and pathways that play a key role during infection.

GOMiner — To provide additional statistical stringency to the identification of potential targets, we then analyzed the data sets generated by the SAM - RS analysis of the microarray data for hypoxia - associated coregulation of multiple, functionally related genes.

Our goal is to help scientists who want to use this technology for gene editing by summarizing current advances on many technical aspects, from the RNA guide optimized design to the genotyping analysis and the validation of the newly generated models.

A team led by Pablo Tamayo and Jill Mesirov of the Broad Institute and University of California, San Diego, and Broad bioinformatician Arthur Liberzon, has generated «hallmark» gene sets from the Molecular Signatures Database (MSigDB), one of the most comprehensive and widely used databases for gene set enrichment analysis.

To delineate the role of Emk in development and adult tissues, mice lacking Emk were generated by targeted gene disruption.

The goal of the International Mouse Phenotyping Consortium (IMPC) is to discover functional insight for every gene by generating and systematically phenotyping 20,000 knockout mouse strains.

Although CRISPR is incredibly useful for generating mutations by NHEJ and generating small mutations with HDR, when it comes to larger scale genome editing, such as replacement of a mouse gene with its human ortholog (greater than 5 kb), it remains to be seen whether CRISPR is as robust as conventional gene targeting.

The IMPC is generating a knockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse Consortium (IKMC).

The Repository of mouse ES cell lines, strains and vectors generated by the trans - NIH KOMP initiative that aims to generate a public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

The draft genome generated by the present study covered > 70 % of the whole genome and harbored > 87 % of the known gene transcripts, thereby constituting the most informative genome resource currently available for bay scallop.

Reducing the activity of the mitochondria - associated gene clk - 1 - lowering the amount of protein generated from its blueprint in other words - boosts mouse longevity by 30 % or so.

We can do this by putting «backup copies» of the mitochondrial genes into the nucleus, where they can not be damaged by free radicals generated in the mitochondria.

The software, called DECoN, accurately and quickly detects changes in copy number of blocks of DNA called exons, by analysing sequencing data already generated to identify smaller gene changes.

Most VSG genes are pseudogenes, which may be used to generate expressed mosaic genes by ectopic recombination.

CRGGH generated a database of pharmacogenomically - relevant gene variants by mining the 1000 Genomes database and conducting de novo genotyping using the Affymetrix ADME gene chip in 19 global populations sampled from 5 continents (Africa, North and South America, Europe, and Asia) for a total of 1,478 individuals.