The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in
generating microvascular endothelial cell lines from a number of different organs.
We capitalized on one of the innate responses of
microvascular endothelial cells, i.e., the up - regulation of cell adhesion molecules in response to inflammatory agents, to
generate endothelial cell lines from different organs.