Sentences with phrase «generation sequencing methods»

Our aim was to investigate a broad collection of European isolates of C. abortus, using next generation sequencing methods, looking at diversity, geographic distribution and genome dynamics.

I co-led the research team that first sequenced and analyzed a whole cancer genome using next - generation sequencing methods.

Classical pathological, bacteriological, serological, molecular, histological and electron microscopical methods were combined with high throughput microarray and next generation sequencing methods to undertake the most extensive and exhaustive evaluation of the cause of death of any zoo animal to date.

The TrueMethyl Whole Genome (TMWG) integrated workflow system for processing DNA samples includes all - in - one reagents for sample conversion, library creation, and indexing, combined with bioinformatics tools to enable accurate DNA modification analysis through next - generation sequencing methods.

The new study, led by Johannes Krause, a geneticist at the Max Planck Institute for the Science of Human History in Jena, Germany, used next - generation sequencing methods to read stretches of any DNA present in a sample and fish out those that resembled human DNA.

That's why the next - generation sequencing method that the 1000 Genomes Project has been doing doesn't pertain to this region.»

The next - generation sequencing method (NGS) was performed on the MiSeq platform.

Not exact matches

Three studies used adequate methods for random sequence generation, but their methods for allocation concealment were not clear (Paul 2012; Penfold 2014; Serwint 1996).

Method of sequence generation not described.

Nine studies reported genuine random methods of generation of the randomisation sequence (Begley 2011; Biro 2000; Harvey 1996; Hicks 2003; Homer 2001; MacVicar 1993; McLachlan 2012; Rowley 1995; Turnbull 1996).

Methods used for generation of the randomisation sequence were described for each trial.

«This method utilizes long PCR, or long - range PCR, to strategically generate DNA templates for next - generation sequencing,» explains Simon Uribe - Convers, graduate student and lead author.

One current method commonly used today is called chromatin immunoprecipitation, combined with next - generation gene sequencing (ChIP - seq).

His laboratory develops and deploys new biochemical and computational methods in functional genomics, to elucidate the genetic basis of human disease and human physiology, and to create and deploy novel techniques in next - generation sequencing and algorithms for tumor evolution, genome evolution, DNA and RNA modifications, and genome / epigenome engineering.

They then used next generation sequencing — a state - of - the - art method to rapidly measure gene expression — to sequence and quantify the thousands of genes that are expressed in hair cells, in comparison with other cells in the ear.

And they figured they could read the address using methods akin to next - generation DNA sequencing, a set of high - speed genome sequencing methods Church helped develop in the early 2000s.

Existing technology, known as next - generation sequencing (NGS), measures genomes derived from millions of cells versus the newer method for single - cell sequencing, called Monovar.

Therefore, in 2015, the DNA was extracted from the teeth and, following hybridization capture of the mitochondrial and Y chromosome fractions, sequenced by a next generation method.

Reference materials are critical to properly evaluate the next - generation of gene sequencing and genetic testing methods that will increase the reliability and effectiveness of precision medicine (also known as «personalized medicine»), in which a person's genetic profile is used to create treatments and therapies unique to that individual.

Using next - generation sequencing, the U.K. team showed that the iPS cells» genome was «clean» — the gene - editing methods had not added any new mutations.

They developed a pipeline that combined fosmid - pooling strategy with whole - genome shotgun strategies, based solely on next generation sequencing (NGS) and hierarchical assembly methods.

ngsTools: methods for population genetics analyses from next - generation sequencing data.

However, the high costs of sample preparation for high - throughput next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes like plasmid validation.

The Sanger Institute exhibit, «DNA Sequencing: Beyond the Genome,» took visitors on a journey through the history of DNA sequencing from early methods through to powerful next generation tecSequencing: Beyond the Genome,» took visitors on a journey through the history of DNA sequencing from early methods through to powerful next generation tecsequencing from early methods through to powerful next generation technologies.

Sanger sequencing methods represent the most popular in a range of «first - generation» nucleic acid sequencing technologies.

There have been tremendous advances in genetic technology, next - generation sequencing, and also, high - throughput methods to genotype patients, and get genetic information.

Newer methods, which leverage massively parallel sequencing approaches, are often referred to as Next - Generation Sequencsequencing approaches, are often referred to as Next - Generation SequencingSequencing (NGS).

The introduction of next generation sequencing (NGS) in 2010 as a diagnosis method for patients was a great accelerator in rare disease gene discovery.

These methods include CRISPR technology, statistical analyses, microfluidics, next - generation sequencing, and systematic mapping.

New results from a research group lead by SciLifeLab faculty member Richard Rosenquist Brandell shows that next - generation sequencing technology can provide equally reliable results as previous methods.

Her laboratory focuses on (1) the use of next generation sequencing to define the microbiome and host immunologic features in patients with human diseases and (2) developing custom computational tools for the identification of novel human commensals and pathogens in these inpatient populations, and (3) using statistical and functional biological methods to understand the complex interplay between the human microbiome and host biology.

A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR - generated baits

Here, we present a method, called MrMosaic, to detect structural mosaic abnormalities using deviations in allele fraction and read coverage from next - generation sequencing data.

In addition to standard clinical and molecular methods for influenza typing, next generation sequencing and subsequent de novo genome assembly were performed to investigate the phylogeny of the collected patient samples.

Next - generation sequencing involves the application of glass micro-chip based methods and small - volume liquid handling (microfluidics) to sequence DNA more quickly and more cheaply than ever before, indeed 1000s times less costly than the technology used to sequence the first human genome just a few years ago.

33/4: 00 A method for detecting intragenic copy number changes of BRCA1 and BRCA2 using next - generation sequencing data.

ASHG 2011: Next - Generation Genome Sequencing Methods Reveal New Findings on Evolution and Population Genetics at ICHG / ASHG 2011 Meeting ASHG Press Briefing - October 13, 2011

«In this study we applied the method to bacterial 16S sequencing but since the generation of these libraries is easy, flexible and cost effective — no fancy fluidics equipment or chemical synthesis of specific barcodes is needed — we are currently applying the assay in a number of other projects.

Using high - throughput ChIP - Seq and chromatin property data, we are developing a new generation of bioinformatics methods for the identification of regulatory sequences.