Sentences with phrase «generation sequencing of»

Next - generation sequencing of the PCR products is used to identify DNA sequences specific to previously identified mutations detected at diagnosis.

It will make use of cutting - edge approaches in vivo and in vitro, including mouse genetic models and next - generation sequencing of RNA and immunoprecipitated chromatin (RNA - seq and ChIP - seq).

Elise Huchard, Christina Albrecht, Susanne Schliehe - Diecks, Alice Baniel, Christian Roos, Peter M. Kappeler, and Markus Brameier, «Large - scale MHC class II genotyping using next generation sequencing of a wild lemur population», Immunogenetics, vol.

Next - Generation Sequencing of the Bacterial 16S rRNA Gene for Forensic Soil Comparison: A Feasibility Study — Ellen M. Jesmok — Journal of Forensic Sciences — February 2016

Next generation sequencing of small RNA confirmed global suppression of miRNA and endogenous siRNA synthesis in ADAR1 - null mouse embryos.

They identified ALK - positive mesotheliomas by immunohistochemistry; confirmed with fluorescence in situ hybridization; and performed targeted next - generation sequencing of tumor DNA and RNA to get a full picture of the exact genetic rearrangement underpinning the disease.

Main Outcomes and Measures Stool microbiome composition was characterized using next - generation sequencing of the 16S rRNA gene.

Stool microbiome composition was characterized using next - generation sequencing of the 16S rRNA gene.

Not exact matches

Once you have a scalable source of lead generation, an effective sales process and an autoresponder sequence, to nurture leads and turn them into sales, you have to be able to track the results.

Unlike genotyping, which just looks for specific parts of DNA and pieces them together, next - generation sequencing looks at only the protein - encoding parts of your genome, called the exome.

The test — which currently costs $ 99.95 but originally was $ 199.95 — is different from AncestryDNA and 23andMe in that it uses next - generation sequencing instead of genotyping technology.

The next - generation sequencing analyzes roughly 2 % of those 3 billion base pairs.

By using Next - Generation sequencers, Dr. Yasuoka decoded frog DNA sequences bound by those proteins and successfully created a genetic map of protein - binding regions in the genome in a process called mapping.

The recurrent biblical phrase «these are the generations of...» emphasizes humankind's place and responsibility within the sequence of history.

The fourth distinctive characteristic is a biological organism that can not survive unless sustained by the resources of a complex culture, therefore an organism that has evolved not like other organisms by eliminating the unfit in the struggle with the physical environment, but by eliminating those organisms unfit to absorb and sustain the complexities of a culture progressively accumulated through the sequence of many generations.

History is the accumulation of the resources for human living gathered through a long sequence of generations and delivered to the present to make us what we are.

How far this creativity can go in creating the human level in the case of any one individual depends partly upon his innate capacity but most of all upon two other features: (1) how wide and deep is the volume of history that reaches him, that is, how abundant and coherent are the values that have been accumulated in the history he inherits and (2) how deep is the communion he is able to have with other persons who embody these meanings accumulated through a long sequence of generations.

Join NSF AuthenTechnologies as we discuss Next Generation Sequencing (NGS), chromatographic confirmation of botanical identity and the DNA Authenticated Verification program with help from NSF laboratory experts.

We scored risk of bias related to random sequence generation, allocation concealment, and selective reporting as unclear for the majority of the studies due to lack of information.Our primary outcome was pain.

The researchers used next generation sequencing technology, RNA sequencing, to reveal «in exquisite detail» the blueprint for making milk in the human mammary gland, according to Laurie Nommsen - Rivers, PhD, RD, IBCLC, a scientist at Cincinnati Children's and corresponding author of the study, published online in PLOS ONE, a journal of the Public Library of Science.

For the purpose of this review, we defined «high quality» as a trial having adequate sequence generation, allocation concealment and an attrition rate of less than 20 %.

Method of sequence generation not described.

Nine studies reported genuine random methods of generation of the randomisation sequence (Begley 2011; Biro 2000; Harvey 1996; Hicks 2003; Homer 2001; MacVicar 1993; McLachlan 2012; Rowley 1995; Turnbull 1996).

Methods used for generation of the randomisation sequence were described for each trial.

The researchers performed state - of - the - art next generation sequencing (NGS) on a model of human deafness to identify the critical lncRNAs.

«It can handle multiple biomarker technologies simultaneously, such as complex flow cytometry, next - generation sequencing, immunosequencing, epigenetic profiling, and other types of asssys measuring biological variation as well,» says Marshall.

«Such losses are particularly critical in the auditory system, because they reduce the temporal precision of signal transmission — and the quality of our perception of the acoustic environment is primarily dependent on rates of action potential generation and precise neural computation of their temporal sequences.»

Next - generation sequencing and global data sharing challenge many of the governance mechanisms currently in place to protect the privacy of research participants.

To test their hypothesis, the researchers used next - generation sequencing (NGS) technology to sequence the complete set of coding variants from the genomes of individuals from seven populations within and outside Africa, i.e. from the Democratic Republic of Congo, Namibia, Algeria, Pakistan, Cambodia, Siberia and Mexico.

From Sewers to Streetlights, Microbes Are Grabbing Civil Engineers» Attention An understanding of how to cultivate a healthy «microbiome» in our living areas is now getting off the ground, boosted by next - generation DNA sequencing technologies.

This quantitative, targeted, multiplexed approach leverages the power of next - generation sequencing and PCR technologies, and allows the researcher to obtain a molecular snapshot of the tumor microenvironment.

A patients» tumors from cholangiocarcinoma, a rare liver cancer that typically fails chemotherapy, shrunk significantly after treatment with FGFR inhibitors, which were prescribed based on results of next generation sequencing.

«Next generation sequencing tools were used to profile patients» tumors,» said Razelle Kurzrock, MD, director of the Center for Personalized Cancer Therapy at UC San Diego Moores Cancer Center.

«Our work demonstrates that the generation of genome sequences from a large number of archaic human individuals is now technically feasible, and opens the possibility to study Neandertal populations across their temporal and geographical range,» says Janet Kelso, the senior author of the new study.

It's well established, of course, that environmental effects like radiation, which alter the genetic sequences in a sex cell's DNA, can leave a mark on subsequent generations.

The next generation of sequencing technology will take results to a new level

But faster next - generation sequencing technology lets researchers sequence a much larger portion of the antibody arsenal.

«It's also able to run the latest in cutting - edge genetic analysis called Next Generation Sequencing, which a lot of researchers are currently utilizing.

The new study, led by Johannes Krause, a geneticist at the Max Planck Institute for the Science of Human History in Jena, Germany, used next - generation sequencing methods to read stretches of any DNA present in a sample and fish out those that resembled human DNA.

Apart from using a host of cutting - edge molecular techniques such as Next Generation Sequencing or NGS, the researchers also had to develop specific bioinformatic tools for analysing large amounts of genomic data.

The FEMTO Pulse is designed to meet the needs of next - generation sequencing (NGS) systems analyzing ever - longer DNA fragments.

The final evidence: Archaeological geneticist Turi King extracted DNA from a tooth and compared its sequence to samples from two of Richard III's living relatives, 17 generations down the line.

The researchers then compared the expression of all genes in six parts of the brain of the two bird species using state - of - the - art molecular techniques, including next - generation sequencing — the first time these tools have been used to find brain properties related to innovation and problem - solving in wild birds.

If the mutations prove harmful, some plants in the next generation revert to their grandparents» DNA sequence with the help of the RNA.

Thus in the early generations, most of the machines bumbled about randomly, but gradually, as «mutations» made changes in the robots» behavior, some of them stumbled onto productive sequences of behaviors.

The authors of this study have developed a universal primer set across flowering plants that amplifies 3 - 15 kilobase fragments, which can then easily be sequenced using recently developed next - generation sequencing technologies.

With the continual improvement of next - generation sequencing technologies, however, obtaining large molecular data sets is becoming much easier, and much cheaper.

«This study is remarkably comprehensive, which will help any molecular laboratory design and implement their own next - generation sequencing lymphoma panel using this work as a template,» commented Robert S. Ohgami, MD, PhD, Assistant Professor of Pathology at the Stanford University Medical Center, in an accompanying editorial.

The team has also found that in hothead mutants, other faulty genes mysteriously revert to the sequence of earlier generations too.

Kelley and his team also used mice with fluorescent markers in different cells of the ear followed by next generation sequencing.