Sentences with phrase «genes in cells infected»

By stymieing three genes in cells infected with HIV, researchers stop the virus from spreading to other cells

Not exact matches

The overlap in gene expression changes when neural progenitor cells are infected by African or Asian strains of Zika virus.

«We hypothesized that individual mutations in viral genes could be expected to have a range of effects on the virus's ability to replicate, to infect new cells and escape the immune system,» Carlson says.

The virus, redesigned using sophisticated protein engineering techniques, works: With its shield and its adapter, these viral gene shuttles efficiently infected tumor cells in laboratory animals.

By studying infected cells grown in a laboratory, the team found that a large number of CMV's genes help it hide from the immune system by allowing it to destroy many of the proteins produced by the body during virus infection and preventing them from activating immune cells to destroy the virus.

Epigenetic therapies are thought to work in two ways to fix these errors in cancer cells — by correcting the «position» of the gene switches and by making the cell appear as though it's infected by a virus, triggering the immune system.

Even if this did happen in the body, the resulting virus would resemble HIV, as the hybrid contains no Ebola genes — and HIV can not infect lung cells.

The virus can not infect noncancerous cells, Kirn explained, because researchers deleted its thymidine kinase gene, which it needs to replicate in the body.

With chronically infected mice as their model, the researchers used a new technology called ATAC - seq to map the regulatory regions of the genome — the sections of DNA involved in switching genes on and off — in the animals» exhausted and functional CD8 + T cells.

Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.

The team is the first to demonstrate that HIV - 1 replication can be completely shut down and the virus eliminated from infected cells in animals with a powerful gene editing technology known as CRISPR / Cas9.

In clinical trials already underway, for example, researchers have used an older gene - editing technique, enzymes call zinc finger nucleases, in immune cells to deactivate the gene for CCR5, a surface protein that HIV latches onto in order to infect cellIn clinical trials already underway, for example, researchers have used an older gene - editing technique, enzymes call zinc finger nucleases, in immune cells to deactivate the gene for CCR5, a surface protein that HIV latches onto in order to infect cellin immune cells to deactivate the gene for CCR5, a surface protein that HIV latches onto in order to infect cellin order to infect cells.

The research showed the beta - lactamase genes in Klebsiella pneumoniae were on all the time, whether or not the bacteria were infecting human cell cultures.

Of course, it makes sense that viruses would choose to turn off genes that the immune system needs to fight the virus, «like interferon - b, which is a highly anti-viral gene expressed in virtually all cell types; or genes that T cells need to recognize virus - infected cells,» Kuss - Duerkop says.

Niles» team was able to disrupt this gene in 100 percent of parasites treated with the CRISPR system; red blood cells infected by those parasites remained smooth.

Moving their studies from cells to whole animals, the researchers tested the effects of knocking out the Zbp1 gene in mice infected with influenza.

A quantitative assay of the suppressor tRNA activity in these mammalian cells is described; it is based on the amount of tRNA - mediated readthrough of a terminating codon in the influenza virus NS1 gene after the cells are infected with virus.

In 2009, for example, Sangamo Therapeutics in Richmond, California, began using zinc finger nucleases to modify genes in immune cells from HIV - infected people, hoping to make the cells resistant to the viruIn 2009, for example, Sangamo Therapeutics in Richmond, California, began using zinc finger nucleases to modify genes in immune cells from HIV - infected people, hoping to make the cells resistant to the viruin Richmond, California, began using zinc finger nucleases to modify genes in immune cells from HIV - infected people, hoping to make the cells resistant to the viruin immune cells from HIV - infected people, hoping to make the cells resistant to the virus.

They did so using a technique called RNA interference, or RNAi, to clamp down on three genes found in infected cells, blocking the wily virus from moving to other cells.

Because they carry mutations in a key immune system gene, many of these cells are infected with flu viruses (green).

The researchers used RNAi to block three genes: two found in the virus itself and one found in mouse T cells — the primary immune system cells infected by the virus.

She found a gene for what's known as the major histocompatibility complex (MHC)-- cell surface molecules that help the immune system recognize foreigners — that was remarkably similar to one in humans that allows infected people to keep the virus in check for decades.

In tests using human neural progenitor cells (NPCs)-- self - renewing, multipotent cells that generate neurons and other brain cell types — the scientists found that exposure to sofosbuvir not only rescued dying NPCs infected with the Zika virus, but restored gene expression linked to their antiviral response.

Another, previously unknown gene — when the investigators worked out its protein's structure and studied it in tissue culture — proved to do just the opposite: The protein helped the schizont leave the infected blood cell.

That gene, HLA - B * 5701, codes for a protein on immune cells that plays a central role in clearing the body of HIV - infected cells.

(B, C) Flow cytometric analysis of HIV - 1 gene expression in (B) mock infected or (C) latently infected CD4 + T cells under non-polarizing conditions, either at the basal state or after reactivation with antibodies against CD3 and CD28.

Previously we engineered zinc - finger nucleases (ZFNs) to specifically disrupt the CCR5 gene in primary human T cells, the predominant cell type infected and killed by HIV.

Second, the AAV preps generally used for gene editing are low titre, and are therefore able to infect only a small subset of the cells used in a given experiment.

The use of viral vectors in research is beneficial for a number of reasons, including but not limited to: helping to get difficult - to - deliver DNA into mammalian cells, increasing the efficiency of gene transduction, allowing for control over which cells are infected through viral pseudotyping, and ease of vector cloning and modification.

The researchers used the new technique to mutate the genes CXCR4 and CCR5, which encode receptor molecules that different strains of the HIV virus use to sneak in and infect immune cells and which have been targeted in previous cell therapy trials.

The first reports that gene - editing of bone marrow stem cells in monkeys infected with a variant of simian immunodeficiency virus (SIV) significantly reduces the number of dormant «viral reservoirs» that pose a risk of reactivation.

Characterization of varicella - zoster virus gene 21 and 29 proteins in infected cells.

The first reports that gene - editing of bone marrow stem cells in monkeys infected with a variant of simian immunodeficiency virus (SIV) significantly reduces the number Read more about Gene - editing and vaccines could improve HIV treatment - Scgene - editing of bone marrow stem cells in monkeys infected with a variant of simian immunodeficiency virus (SIV) significantly reduces the number Read more about Gene - editing and vaccines could improve HIV treatment - ScGene - editing and vaccines could improve HIV treatment - Scimex

The third Objective (IRF3) brings together promising scientists and clinicians from the University of Pennsylvania and the Rockefeller University to combine gene therapy strategies, independently tested in humans, with the goal of engineering, growing, and administering killer cells that are uniquely empowered to find and kill HIV - infected cells.

This type of «killer» T cell responds to previously encountered cell - surface molecules — including the fragments of SIV proteins encoded by the genes in the CMV / SIV vaccine — and destroys SIV - infected cells.

Now, a novel computational method shows that a viral protein found in EBV - infected human cells may activate genes associated with increased risk for autoimmunity.

In 2007, a research group at Children's Hospital of Philadelphia (CHOP) led by Jean Bennett began inserting a working version of the RPE65 gene into the eye by means of a harmless virus that carries the gene and «infects» the DNA of the RPE cells.

JJ Miranda, PhD, reported that certain drugs affect genes in tumor cells infected with the Epstein - Barr Virus.

But by placing the gene under the control of a «promoter» — an on - off switch for gene expression — from a gene (SM22a) that is expressed only in smooth muscle cells, Dr. Parmacek's team found that no matter which types of cells were infected, the gene was only «turned on» in smooth muscle cells.

In a study published in the journal Biochemical and Biophysical Research Communications, a team led by Miranda examined how certain drugs affect genes in EBV - infected tumor cellIn a study published in the journal Biochemical and Biophysical Research Communications, a team led by Miranda examined how certain drugs affect genes in EBV - infected tumor cellin the journal Biochemical and Biophysical Research Communications, a team led by Miranda examined how certain drugs affect genes in EBV - infected tumor cellin EBV - infected tumor cells.

Developed by Kotin et al. (37), the BEVS uses Spodoptera frugiperda (Sf9) cells infected with recombinant baculoviruses containing the vector genome, helper genes, and capsids to generate yields of 1014 — 1016 viral genomes per liter in 100 - L to 200 - L bioreactors (4, 38).

These reporters will give us the ability to sort infected cultures to analyze changes in gene expression in directly infected cells and bystander cells and compare to uninfected cells.

Partial block to transcription of human adenovirus type 2 late genes in abortively infected monkey cells

First cloning of the gene for prion protein (PrP), demonstration that PrP knockout mice are resistant to prion disease and that PrP antibodies abrogate prions in infected cells.

We combine biochemical, structural, cellular and functional information using purified proteins, mutant and transgenic plants, yeast and chemical genomic screening systems, transient gene expression assays, confocal microscopy and in silico data analysis to compare ROP - centered kinase signaling during cell polarity (in vitro pollen tubes), morphogenesis (whole plant) and pathogenesis (fungi - infected cells).