Sentences with phrase «genes in mouse cells»

Not exact matches

Using the gene - editing tool CRISPR - Cas9 to turn off certain genes in a mouse zygote as well as other new techniques to enrich the pluripotent stem cells of a rat, the group managed to grow various rat organs (a pancreas, heart, and eyes) in a mouse embryo.

@harleybird When you mutate many genes in a few cells (or even in all of the cells) within a mouse, you would not expect to create a new mouse.

But, in mice where the gene couldn't be activated in the gut, TH17 cells that were exposed to bile acids suffered severe oxidative stress.

In experiments with mice, the researchers found that Paneth cells engineered to lack a functional ATG16L1 gene were five times more likely to die in the face of rising TNF - alpha signals than normal cellIn experiments with mice, the researchers found that Paneth cells engineered to lack a functional ATG16L1 gene were five times more likely to die in the face of rising TNF - alpha signals than normal cellin the face of rising TNF - alpha signals than normal cells.

The researchers corrected a mutation in liver cells in mice by snipping out the gene and sewing in a healthy copy of it.

The method repaired the gene in just one in 250 mouse liver cells, but those cells replicated enough to break down tyrosine and cure the disorder.

These experiments were complemented by genetic manipulations in which some mice were engineered to lack a gene known as Tap1, which is crucial for the MHC I complex to make its way to the cell surface.

When similar analysis was performed on the db mice, it was found that the disrupted db gene was responsible for encoding a protein that functions as a leptin receptor: When it binds circulating leptin at the cell surface, it sets in motion a biochemical cascade inside the cell.

Researchers have pinpointed a gene that keeps important brain cells in mice from crossing their wires, providing a possible link between brain wiring and mood disorders like depression.

So far, gene therapy attempts have only resulted in partial improvements of hearing in mouse models of specific human deafness forms that did not include severe anomalies in hair cell structure.

In 2006, he used retroviruses to insert four genes into the chromosomes of mouse skin cells.

They validated the cell models and showed that changing clock gene function in these cells is similar to what happens in mice lacking clock genes.

Researchers then tested cell cultures and mouse models by using a gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect cell fusion and muscle formation.

Base oxidation regulates gene activity In cooperation with colleagues at LMU, as well as researchers based in Berlin, Basel and Utrecht, Carell and his group have now shown, for the first time, that a standard base other than cytosine is also modified in embryonic stem cells of micIn cooperation with colleagues at LMU, as well as researchers based in Berlin, Basel and Utrecht, Carell and his group have now shown, for the first time, that a standard base other than cytosine is also modified in embryonic stem cells of micin Berlin, Basel and Utrecht, Carell and his group have now shown, for the first time, that a standard base other than cytosine is also modified in embryonic stem cells of micin embryonic stem cells of mice.

Since genes for the T - cell receptor beta chain were previously shown to be on mouse chromosome 6, all three of the Ig - like multigene families expressed and rearranged in T cells are located on different chromosomes, just as are the B - cell multigene families for the Ig heavy chain, and the Ig kappa and lambda light chains.

When the huntingtin gene is deleted at an age older than four months, these mice appeared to stay healthy, despite having lost their huntingtin genes in cells all over their bodies.

In today's issue of Cell, a team reports that it has found in mice and humans a close relative of a fruit fly clock gene — the first evidence that some of these genes may have been conserved over the course of evolutioIn today's issue of Cell, a team reports that it has found in mice and humans a close relative of a fruit fly clock gene — the first evidence that some of these genes may have been conserved over the course of evolutioin mice and humans a close relative of a fruit fly clock gene — the first evidence that some of these genes may have been conserved over the course of evolution.

The researchers used the dead guide RNAs to turn on the Pdx gene in the mice's livers, which caused the liver cells to produce insulin, reversing the mice's diabetes.

The researchers have compared various processes involved in gene expression, such as gene transcription and chromatin modification, and have repeated this in different tissues and cell types from both humans and mice.

The lack of these genes in the neurons of active mice suggested that their brain cells did not immediately leap into an excited state in response to the stressor.

Since patients (and mice) with Usher 1c also have balance problems caused by hair - cell damage in the vestibular organs, the researchers also tested whether gene therapy restored balance.

Moreno and his team already have shown that when cell - competition genes are knocked out in mice, cancer growth is inhibited.

The animals were five times as likely as regular mice to go into shock and die when exposed to bacterial cells, the group reports in the November 15 issue of Genes and Development.

The UT Southwestern group had previously used CRISPR - Cas9, the original gene - editing system, to correct the Duchenne defect in a mouse model of the disease and in human cells.

Nadeau stumbled upon one study, in mice, describing how environmental factors can tag Foxp3 with chemical markers that tell T - cell precursors to switch the gene on or off.

To determine the effect of mutations that reduce TET2 function in abnormal stem cells, the research team genetically engineered mice such that the scientists could switch the TET2 gene on or off.

Using the new gene - editing enzyme CRISPR - Cpf1, researchers at UT Southwestern Medical Center have successfully corrected Duchenne muscular dystrophy in human cells and mice in the lab.

Once the UCLA researchers had produced iPS cells that were free from Duchenne mutations, they differentiated the iPS cells into cardiac muscle and skeletal muscle cells and then transplanted the skeletal muscle cells into mice that had a genetic mutation in the dystrophin gene.

Already, researchers have used CRISPR / Cas9 to edit genes in human cells grown in lab dishes, monkeys (SN: 3/8/14, p. 7), dogs (SN: 11/28/15, p. 16), mice and pigs (SN: 11/14/15, p. 6), yeast, fruit flies, the worm Caenorhabditis elegans, zebrafish, tobacco and rice.

And by studying mice lacking the gene for ERRγ (and therefore unable to make the ERRy molecule), the team observed that all brown fat cells resembled white cells in these mice.

In the current work, they used a new variation of the gene - editing system to repair the defect in both a mouse model and in human cellIn the current work, they used a new variation of the gene - editing system to repair the defect in both a mouse model and in human cellin both a mouse model and in human cellin human cells.

Using a mouse model, the team also demonstrated that two processes during neurodevelopment are regulated by the gene: proliferation — the replication of neuronal stem cells that have the potential to become multiple different kinds of cells, including neurons — and migration — the movement of neurons to specific locations in the brain during development.

Scientists have known for 20 years that SMN is necessary in every cell of the body, since disrupting the gene in a mouse causes early embryonic death, before muscle or nerve cells form.

In one such study by Ronald Evans and colleagues, the gene for rat growth hormone is stably inserted into mouse cells by a retrovirus.

They destroyed the T cells in 12 mice, five of which received marrow cells from normal mice while seven received marrow from mice with a defective Fas - ligand gene.

Visel and his colleagues studied gene expression in a developing mouse embryo, and found 120 enhancers active in cells of the face.

But researchers have found a mutation in a mouse gene that leads to an arthritis - like condition because it causes the joint's cartilage cells to pump insufficient amounts of pyrophosphate — a natural water softener — into the joint cleft.

Sensory hair cells in the cochlea of a Beethoven mouse treated with TMC2 gene therapy.

The control mice, with all genes intact, should have lost sight as photo - receptors — the light - sensitive cells in the retina — died.

The team also found that ERAS, a tumorigenic gene expressed in mouse embryonic stem cells and iPSCs, was mutated and dysfunctional in the mole - rat iPSCs.

Initial tests on mice showed the hybrid virus was very efficient: the gene it carried was active in 24 per cent of airway cells after two months, a far better proportion than achieved by other delivery methods (New Scientist, 10 March 2001, p 19).

First author Kim Martinod, a graduate student in the Immunology Graduate Program at the Harvard University Medical School, found that, in response to vein constriction, these «rescued» mice now could function normally, forming clots as efficiently as mice with a functioning Pad4 gene, demonstrating that the Pad4 gene did produce a functioning PAD4 enzyme in these white blood cells to regulate blood clotting.

In a related paper published online today in Nature Biotechnology, Konrad Hochedlinger of the Harvard Stem Cell Institute in Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipIn a related paper published online today in Nature Biotechnology, Konrad Hochedlinger of the Harvard Stem Cell Institute in Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipin Nature Biotechnology, Konrad Hochedlinger of the Harvard Stem Cell Institute in Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipin Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tipin mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tips.

To do that, they deleted the gene for SIRT1, which encodes the major mammalian sirtuin, in endothelial cells of mice.

The researchers scoured the already deciphered mouse genome, looking for genes that might encode additional receptor proteins in its olfactory system, the sensory cells that connect the nose to the brain.

However, cancer cells may instead be coaxed to turn back into normal tissue simply by reactivating a single gene, according to a study that found that restoring normal levels of a human colorectal cancer gene in mice stopped tumor growth and re-established normal intestinal function within only 4 days.

Unleashing RNA molecules against hepatitis B genes protects liver cells in mice (right).

The group has already started tweaking human iPS cells using the same genes that Saitou pinpointed as being important in mouse germ - cell development, but both Saitou and Hayashi know that human signalling networks are different from those in mice.

When researchers suppressed the ARF gene in mole - rat cells during the reprogramming process to iPSCs, the cells stopped proliferation with sign of cellular senescence, while the opposite happens with mouse cells.

Decades of work in developmental biology have provided a start: Biologists have used mutant frogs, flies, mice, chicks, and fish to identify some of the main genes that control a developing cell's decision to become a bone cell or a muscle cell.