Sentences with phrase «genes in the cell lines»

In all, the study found and validated 76 alterations in cancer - associated genes in the cell lines, many of which are involved in activating known oncogenes including TERT, TP53 and PIK3CA.

Investigating these genes in cell lines and genetically modified stem cells is a natural approach.

Not exact matches

For example, if I come from a long line of brown - eyed people, can I aim to alter the DNA molecules in my reproductive cells so as to pass on to my son «genes for blue - eyedness»?

Where is the clear line in a progression from (1) using animal insulin to treat diabetes, to (2) using gene remodeling techniques to grow insulin in a host bacterium that will reproduce rapidly and from which a plentiful supply of insulin can be harvested, to (3) genetic surgery to replace the defective gene in a person diagnosed as diabetic, to (4) genetic surgery immediately after fertilization in order to replace the defective gene and alter the germ cells which would otherwise have transmitted the disease to one's offspring?

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

According to Izpisúa Belmonte, who is also a professor at the gene expression laboratories of the Salk Institute for Biological Studies in San Diego, California, CMRB aims to become «a research centre of excellence in south Europe in the line of world - recognized institutions such as the Salk or the Whitehead institutes, where both pre - and postdoctoral researchers receive multidisciplinary training of the highest quality» in stem cell biology and cell regeneration.

Until researchers like Razin came along, the basic story line on how genes get transcribed in a cell was neat and simple.

The second duplication appears in supplemental figure S6, where a scatterplot purporting to show gene expression similarities between the cell lines was used twice.

«The bottom line question,» says Christopher Austin, a neurogeneticist at Merck Research Laboratories in West Point, Pennsylvania, is whether this kind of gene therapy can stem the rate of brain cell death.

The researchers replicated these three PKC alpha gene variants in laboratory cell lines.

Further experiments showed that each iPS cell line had a different pattern of DNA methylation, a molecular coating of the DNA that turns genes on or off in the cell.

With help from researchers at Duke University, they showed that the gene has a unique pattern of methylation in breast cancer cell lines that might one day help doctors spot the disease.

Several different experiments localized the active gene and its protein to stem cells buried within the crypts, or deep grooves in the intestinal lining.

In the current study, the researchers report that NOTCH1 acts like a sensor on the endothelial cells — the cells that line the valve and vessels — detecting blood flow outside of the cell and transmitting information to a network of genes inside the cell.

In the fetal liver hybridomas, which were constructed from fetal liver cells and a tumor cell, no light chain gene rearrangement was observed, whereas in the 70Z / 3 cell line a kappa light chain rearrangement probably occurreIn the fetal liver hybridomas, which were constructed from fetal liver cells and a tumor cell, no light chain gene rearrangement was observed, whereas in the 70Z / 3 cell line a kappa light chain rearrangement probably occurrein the 70Z / 3 cell line a kappa light chain rearrangement probably occurred.

A gene called CHL1 was produced at lower levels in the most responsive cell lines and at higher levels in the least responsive cell lines.

As part of this effort, Dr. Weinstein and his colleagues inserted a gene for a protein that turns green the cells that line the endothelium of selected embryonic veins and in the lymphatic system — the network of vessels through which immune cells travel in the body.

Researchers showed that the genes turned on by the switch remained active for several hours in several mammalian cell lines even after a short light pulse.

So far, Menendez and his colleagues have discovered the fatty acid cuts the expression of the gene in ovarian, stomach, and colon cancer cell lines.

In one study, geneticist Joseph Ecker at the Salk Institute in California took various stem cell lines reprogrammed from skin, fat, and other tissues and examined each line's genome for dna methylation, chemical marks that alter how genes are expresseIn one study, geneticist Joseph Ecker at the Salk Institute in California took various stem cell lines reprogrammed from skin, fat, and other tissues and examined each line's genome for dna methylation, chemical marks that alter how genes are expressein California took various stem cell lines reprogrammed from skin, fat, and other tissues and examined each line's genome for dna methylation, chemical marks that alter how genes are expressed.

After exploiting a technology that allowed them to activate each of nearly 16,000 genes individually in human melanoma cell lines containing mutant BRAF, the authors then treated the panel of cells with the drugs and monitored which cells showed altered drug sensitivity.

«While we expected to find some mutations in stem cell lines, we were surprised to find that about five percent of the stem cell lines we analyzed had acquired mutations in a tumor - suppressing gene called p53,» said Merkle.

In other organisms, there is instead a signaling mechanism: An embryonic cell receives chemical signals from neighboring cells that activate (or repress) the genes that allow for germ - line function.

The first study to sequence and analyze the entire genome of a HeLa cell line, along with access to its sequence data, has been published Aug. 7 in its final version, by G3: Genes Genomes Genetics, an open - access, scientific journal of the Genetics Society of America.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in micIn the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in micin breast cancer - derived cell lines and in human breast cancers replicated in micin human breast cancers replicated in micin mice.

The team found significant changes in gene expression after light exposure in all cell types in the visual cortex — both neurons and, unexpectedly, nonneuronal cells such as astrocytes, macrophages and muscle cells that line blood vessels in the brain.

The study used whole - exome sequencing to characterize genetic alterations that occur at the single nucleotide level for all genes in 25 cell lines commonly used as models of bladder cancer.

This work, led by Dr Patrick Varga - Weisz shows how chemicals produced by bacteria in the gut from the digestion of fruit and vegetables can affect genes in the cells of the gut lining.

In combination with a separate technique that measures the degree to which a gene is expressed, the researchers then identified genes that were either mutated or functionally altered through expression levels in these bladder cancer cell lineIn combination with a separate technique that measures the degree to which a gene is expressed, the researchers then identified genes that were either mutated or functionally altered through expression levels in these bladder cancer cell linein these bladder cancer cell lines.

And, in the future, the potential to insert barcodes in genes and the development of in - line ID kits, that recognize specific strains of cell lines, could make it easier to verify new organisms and their protein products, and track products through supply chains.

One surprising finding from the study co-led by Johns Hopkins, Ehret says, was that many of the new sites identified were near genes that are active in cells that line the inside of blood vessels, suggesting those cells are somehow involved directly in blood pressure control.

Following up on clues — including the fact that PMS is 56 percent heritable — the NIH researchers studied the genetic control of gene expression in cultured white blood cell lines from women with PMDD and controls.

In addition, progesterone boosted expression of several of these genes in controls, while estrogen decreased expression in cell lines derived from PMDD patientIn addition, progesterone boosted expression of several of these genes in controls, while estrogen decreased expression in cell lines derived from PMDD patientin controls, while estrogen decreased expression in cell lines derived from PMDD patientin cell lines derived from PMDD patients.

An analysis of all gene transcription in the cultured cell lines turned up a large gene complex in which gene expression differed conspicuously in cells from patients compared to controls.

One of the gene areas identified suggests that cells within the lining of the uterus play a larger - than - expected role in the length of pregnancy, which in turn provides a new target for medications to help prevent preterm birth.

It will take more work to confirm that these genes are also differentially active in aging people and not just differently aged cell lines, says aging specialist Leonard Guarente of the Massachusetts Institute of Technology.

In four cell lines derived from human and monkey, siRNAs substantially lowered the expression of three of four test genes — by as much as 90 %.

In the PNAS paper, the scientists demonstrated their method on two genomics problems, the role of gene enhancers in the fruit fly embryo and alternative splicing in a human - derived cell linIn the PNAS paper, the scientists demonstrated their method on two genomics problems, the role of gene enhancers in the fruit fly embryo and alternative splicing in a human - derived cell linin the fruit fly embryo and alternative splicing in a human - derived cell linin a human - derived cell line.

To find methods for stemming the tide of liver - damaging microbes, Schnabl and team tried experimentally bumping up copies of the REG3G gene in intestinal lining cells grown in the lab.

Working with mouse prostate cancer cell lines in the laboratory, the investigators found that cells containing overexpressed TOP2A and EZH2 genes were highly sensitive to attack with a combination of two drugs.

Overall they found that many of these risk genes are associated with the myriad of functions taking place in cells lining blood vessels.

Unlike OCT4, these genes can only be studied in human embryos because they are not expressed the same way, or at all, in mouse embryos or immortalized lines of human stem cells, says her colleague Robin Lovell - Badge, also at the Crick Institute.

Since our collection of cell lines is completely genetically annotated — which means that mutations and expression changes in each line's genes have been documented — we should be able to identify in advance patients who will benefit from specific combinations.

In parthenote - derived cell lines, they found a distinctive signature: Most genes were different on the two sets of chromosomes, but genes located close to the chromosome's center were largely identical.

The results, although still preliminary in a quantitative sense, demonstrate that the reactivation of methylation - silenced genes does occur in different cell lines, suggesting it is a general phenomenon.

To determine whether the reactivation of methylation - silenced genes by EGCG is a general phenomenon that also occurs in other cell lines, we studied the effects of EGCG treatment on the methylation status and mRNA levels of p16INK4a or RARβ in three other human cancer cell lines (Fig. 3C) ⇓.

The protein encoded by the PIK3C2G gene belongs to the phosphatidylinositol -4,5-bisphosphonate 3 - kinase (PI3K) family, which plays a critical role in cancer.28 Experimental evidence suggests that activation of PI3K signaling enhances production of COX - 2 and PGE2, which results in inhibition of apoptosis in colon cancer cell lines that can be restored with NSAID - mediated blockade of PI3K.29

Additionally, the latent provirus integrated in the J - Lat cell lines encodes the GFP gene, providing a fluorescent marker of HIV - 1 transcriptional activity.

Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»

The cell lines will allow scientists to study gene expression in precursor brown fat and white fat cells, and in the mature fat cells these cells create.