Sentences with phrase «genes of each cell line»

Not exact matches

For example, if I come from a long line of brown - eyed people, can I aim to alter the DNA molecules in my reproductive cells so as to pass on to my son «genes for blue - eyedness»?

Where is the clear line in a progression from (1) using animal insulin to treat diabetes, to (2) using gene remodeling techniques to grow insulin in a host bacterium that will reproduce rapidly and from which a plentiful supply of insulin can be harvested, to (3) genetic surgery to replace the defective gene in a person diagnosed as diabetic, to (4) genetic surgery immediately after fertilization in order to replace the defective gene and alter the germ cells which would otherwise have transmitted the disease to one's offspring?

But mice with a working version of the gene suffered little to no damage to their gut - lining cells.

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

According to Izpisúa Belmonte, who is also a professor at the gene expression laboratories of the Salk Institute for Biological Studies in San Diego, California, CMRB aims to become «a research centre of excellence in south Europe in the line of world - recognized institutions such as the Salk or the Whitehead institutes, where both pre - and postdoctoral researchers receive multidisciplinary training of the highest quality» in stem cell biology and cell regeneration.

Some of those matching genes are vital for embryo survival, including several responsible for cell communication, cellular energy and adhesion to the lining of the uterus.

«The bottom line question,» says Christopher Austin, a neurogeneticist at Merck Research Laboratories in West Point, Pennsylvania, is whether this kind of gene therapy can stem the rate of brain cell death.

The viruses had been engineered to be harmless while also smuggling a gene called Atoh1 into cells lining the scala media — the key chamber of the cochlea, containing the hair cells.

His team plans to introduce a healthy copy of the CF gene into cells lining the nose and lungs, using a lipid suspension that fuses with the cell membrane and allows the gene to enter the cell.

Further experiments showed that each iPS cell line had a different pattern of DNA methylation, a molecular coating of the DNA that turns genes on or off in the cell.

Grants issued last summer totaling nearly $ 50 million will go toward producing the first 8,500 or so of the cell lines, each carrying one disabled, or «knocked out,» gene.

With help from researchers at Duke University, they showed that the gene has a unique pattern of methylation in breast cancer cell lines that might one day help doctors spot the disease.

In the current study, the researchers report that NOTCH1 acts like a sensor on the endothelial cells — the cells that line the valve and vessels — detecting blood flow outside of the cell and transmitting information to a network of genes inside the cell.

Two types of immature B cells, namely fetal liver hybridomas and the leukemic cell line 70Z / 3, both of which have cytoplasmic mu chains but no light chains, were examined for DNA rearrangements of their light chain and heavy chain immunoglobulin genes.

The resulting «map» of gene - drug interactions allowed the researchers to accurately predict the responses of multiple human cancer cell lines to different chemotherapy agents based on the cell lines» genetic profiles and also revealed new genetic factors that appear to determine the response of breast and ovarian tumor cells to common classes of chemotherapy treatment.

As part of this effort, Dr. Weinstein and his colleagues inserted a gene for a protein that turns green the cells that line the endothelium of selected embryonic veins and in the lymphatic system — the network of vessels through which immune cells travel in the body.

They used the gene editing technology CRISPR to engineer a series of human embryonic stem cell lines, which were identical apart from the number of DNA repeats that occurred at the ends of their HTT genes.

So far, Menendez and his colleagues have discovered the fatty acid cuts the expression of the gene in ovarian, stomach, and colon cancer cell lines.

After exploiting a technology that allowed them to activate each of nearly 16,000 genes individually in human melanoma cell lines containing mutant BRAF, the authors then treated the panel of cells with the drugs and monitored which cells showed altered drug sensitivity.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

«While we expected to find some mutations in stem cell lines, we were surprised to find that about five percent of the stem cell lines we analyzed had acquired mutations in a tumor - suppressing gene called p53,» said Merkle.

But instead of prodding extracted B cells to proliferate, Wilson says, the teams simply plucked the antibody - producing genes from them and inserted those into existing B - cell lines, thereby increasing their protein output.

The first study to sequence and analyze the entire genome of a HeLa cell line, along with access to its sequence data, has been published Aug. 7 in its final version, by G3: Genes Genomes Genetics, an open - access, scientific journal of the Genetics Society of America.

In all, the study found and validated 76 alterations in cancer - associated genes in the cell lines, many of which are involved in activating known oncogenes including TERT, TP53 and PIK3CA.

Healthy cells (left column) prevent jumping genes such as LINE - 1 from turning on production of their proteins.

The study used whole - exome sequencing to characterize genetic alterations that occur at the single nucleotide level for all genes in 25 cell lines commonly used as models of bladder cancer.

This work, led by Dr Patrick Varga - Weisz shows how chemicals produced by bacteria in the gut from the digestion of fruit and vegetables can affect genes in the cells of the gut lining.

Instead, they show the expression of certain genes and DNA methylations were better indicators of the efficiency at which a cell line could be differentiated into the hematopoietic lineage.

And, in the future, the potential to insert barcodes in genes and the development of in - line ID kits, that recognize specific strains of cell lines, could make it easier to verify new organisms and their protein products, and track products through supply chains.

One surprising finding from the study co-led by Johns Hopkins, Ehret says, was that many of the new sites identified were near genes that are active in cells that line the inside of blood vessels, suggesting those cells are somehow involved directly in blood pressure control.

Following up on clues — including the fact that PMS is 56 percent heritable — the NIH researchers studied the genetic control of gene expression in cultured white blood cell lines from women with PMDD and controls.

In addition, progesterone boosted expression of several of these genes in controls, while estrogen decreased expression in cell lines derived from PMDD patients.

However, more than 90 percent of the cell lines had a strong dependency on at least one of a set of 76 genes, suggesting that many cancers rely on a relatively few genes and pathways.

An analysis of all gene transcription in the cultured cell lines turned up a large gene complex in which gene expression differed conspicuously in cells from patients compared to controls.

One of the gene areas identified suggests that cells within the lining of the uterus play a larger - than - expected role in the length of pregnancy, which in turn provides a new target for medications to help prevent preterm birth.

It will take more work to confirm that these genes are also differentially active in aging people and not just differently aged cell lines, says aging specialist Leonard Guarente of the Massachusetts Institute of Technology.

In four cell lines derived from human and monkey, siRNAs substantially lowered the expression of three of four test genes — by as much as 90 %.

In the PNAS paper, the scientists demonstrated their method on two genomics problems, the role of gene enhancers in the fruit fly embryo and alternative splicing in a human - derived cell line.

To find methods for stemming the tide of liver - damaging microbes, Schnabl and team tried experimentally bumping up copies of the REG3G gene in intestinal lining cells grown in the lab.

Working with mouse prostate cancer cell lines in the laboratory, the investigators found that cells containing overexpressed TOP2A and EZH2 genes were highly sensitive to attack with a combination of two drugs.

Overall they found that many of these risk genes are associated with the myriad of functions taking place in cells lining blood vessels.

Unlike OCT4, these genes can only be studied in human embryos because they are not expressed the same way, or at all, in mouse embryos or immortalized lines of human stem cells, says her colleague Robin Lovell - Badge, also at the Crick Institute.

Since our collection of cell lines is completely genetically annotated — which means that mutations and expression changes in each line's genes have been documented — we should be able to identify in advance patients who will benefit from specific combinations.

We assayed from 6000 to 20,000 shRNAs simultaneously to identify genes important for the proliferation and survival of five cell lines derived from human mammary tissue.

In parthenote - derived cell lines, they found a distinctive signature: Most genes were different on the two sets of chromosomes, but genes located close to the chromosome's center were largely identical.

ReNeuron can generate large numbers of stable cell lines by engineering cells with a modified version of the gene c - myc.

First, we produced PARP1 - KO ovarian cancer cell lines using CRISPR / Cas9 gene editing to test the loss of PARP - 1 as a resistance mechanism to all clinically used PARP inhibitors.

The results, although still preliminary in a quantitative sense, demonstrate that the reactivation of methylation - silenced genes does occur in different cell lines, suggesting it is a general phenomenon.

To determine whether the reactivation of methylation - silenced genes by EGCG is a general phenomenon that also occurs in other cell lines, we studied the effects of EGCG treatment on the methylation status and mRNA levels of p16INK4a or RARβ in three other human cancer cell lines (Fig. 3C) ⇓.

The protein encoded by the PIK3C2G gene belongs to the phosphatidylinositol -4,5-bisphosphonate 3 - kinase (PI3K) family, which plays a critical role in cancer.28 Experimental evidence suggests that activation of PI3K signaling enhances production of COX - 2 and PGE2, which results in inhibition of apoptosis in colon cancer cell lines that can be restored with NSAID - mediated blockade of PI3K.29