Sentences with phrase «genitalium genome»

But synthesizing the M. genitalium genome from the ground up proved challenging, in part because long strands of DNA are quite fragile.

We have synthesized a 582,970 — base pair Mycoplasma genitalium genome.

Not exact matches

To build the minimal genome, Venter turned to a microbe he and his colleagues had already been studying for several years, a pathogen known as Mycoplasma genitalium that causes urinary tract infections.

This synthetic genome, named M. genitalium JCVI - 1.0, contains all the genes of wild - type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection.

In a 1995 Science paper, Venter's team sequenced the genome of Mycoplasma genitalium, a sexually transmitted microbe with the smallest genome of any known free - living organism, and mapped its 470 genes.

(Syn 2.0 was an intermediate stage in this process, the first microbe with a genome smaller than that of M. genitalium, which with 525 genes has the fewest of any free - living natural organism.)

With a total of 531,000 bases, the new organism's genome isn't much smaller than that of M. genitalium, with 600,000 bases.

In January scientists at the J. Craig Venter Institute in Rockville, Md., reported making all 582,970 base pairs that constitute the genome of the bacterium Mycoplasma genitalium.

The synthetic genome was modeled after that of a tiny bacterium called Mycoplasma genitalium, carrying all the same genes in roughly the same order.

Last year, Venter and his colleagues developed a technique for replacing M. genitalium's genome with another natural genome from a different species (Science, 3 August 2007, p. 632).

However, to be sure this genome works as it should, the researchers must still put it into a DNA-less M. genitalium, notes Eckard Wimmer, a molecular virologist at Stony Brook University in New York state: «Proof is biological function, and that's missing in this paper.»

Ever since his group decoded the genome of Mycoplasma genitalium, a parasitic bacterium that lives in the human urogenital tract, sequencing maverick J. Craig Venter has wanted to remake the bug's genome in the lab.

At just under 600,000 bases, M. genitalium sports the smallest known genome for a free - living organism, and Venter hoped that an artificial genome could be modified to turn the bacterium into a living chemical - manufacturing plant.

«The 582,970 base pair M. genitalium bacterial genome is the largest chemically defined structure synthesized in the lab,» lead author Daniel Gibson told ScientificAmerican.com via e-mail.

It is the third mycoplasma to be sequenced, and has the smallest sequenced prokaryotic genome except for M. genitalium.