Sentences with phrase «genome microarray»

Detection of elafin as a candidate biomarker for ulcerative colitis by whole - genome microarray screening

They employed whole genome microarray analysis to perform sample - matched transcriptome analyses.

Parallel whole - genome microarray analysis was used to determine the transcriptome in matched samples.

Related research (Pereira HJ, et al., 2012) used two different lines of evidence to explain the increase in the ratio of anteiso — to iso - branched alkenes that was observed during the transition from early to late stationary phase in M. luteus: structural studies of a key enzyme involved in fatty acid biosynthesis in M. luteus (FabH, or ß - ketoacyl - ACP synthase III) and transcriptional (whole - genome microarray) studies of M. luteus during different growth phases.

Part of this effort involved discovery, through transcriptomic evidence (whole - genome microarrays), that up - regulation of the native fadM thioesterase in E. coli was correlated with enhanced methyl ketone titers.

Not exact matches

The technology for genome - wide profiling using microarrays was newly available, and with it came tremendous excitement, parallel to the mapping of the human genome.

Hughes's winning research proposed using microarray techniques to compare the genomes of a variety of vertebrate animals to test for common regulatory elements that determine gene expression.

The Emory team used high density microarrays to probe more than 900,000 sites across the human genome to detect structural variation, including deletions or duplications of DNA.

We currently produce DNA microarrays representing the yeast, mouse, and human genomes (the yeast microarray has over 6000 yeast genes, the mouse microarray has over 15,000 mouse genes, and the two human microarrays have 1700 genes and over 19,000 genes).

To help identify genes and signaling pathways that may be influenced by zinc, Knoell and his team performed a genome - wide microarray analysis of lung tissue taken from zinc - deficient mice with sepsis.

Xu applied the DNA microarray technique to screen more than 100,000 genes in the human genome to find the exact gene regulation pathway.

Some research teams over the past five years have used microarray or gene - chip technology to compare genomes and quickly scan them for variations of copy numbers on each chromosome.

Using microarrays that enabled them to examine 100,000 sites on the genome, they looked at the cells» nuclear DNA, mitochondrial DNA, and methylation patterns (which give clues to whether a gene has been turned on or off).

Samples of cRNA derived from single animals were hybridized in recommended buffer to microarrays (Murine Genome Array U74Av2, Affymetrix Inc.) at 45 °C for 16 hours.

Band DNA was labeled with Cy5 and hybridized to microarrays with Cy3 - labeled whole genome DNA.

Microarray analysis was performed at different time points to determine yeast genome - wide response when exposed to salt, acid, hypoxic conditions, and hydrogen peroxide, as well as ethanol, isopentenol, and other biofuels candidates.

As mRNA isolation is simple and since DNA microarrays are a proven genomics reagent for monitoring differential gene expression, a genome - wide expression analysis may be the fastest and most efficient method for identifying additional candidate effectors of colony queen number.

Results: Benefiting from the recently sequenced genome of the model sulfate - reducing bacterium (SRB), Desulfovibrio vulgaris, microbiologist Weiwen Zhang and a team of scientists at Pacific Northwest National Laboratory (PNNL) are applying a suite of post-genomics technologies, including microarray, proteomic, and computational analyses, to obtain a broader understanding of D. vulgaris» metabolism.

Comparison of their genomes, integrated with proteomic and microarray data, with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage - specific selective pressures.

The completion of a draft genome sequence for the microorganism Methanosarcina barkeri has enabled use of high - throughput technologies such as microarrays and mass spectrometry of proteins to detect global changes that occur in response to experimental treatments.

The microarrays used in the study were not confined to specific genes only but spanned the entire genome.

Dr. Li's research interests focus on machine learning, bioinformatics, and statistical data mining in large scale data in biomedical research, such as next generation sequencing data (whole genome sequencing, RNA - seq, microarray data), in the file.

We can make cell microarrays that contain a very large percentage of the human genome without being limited by the protein's properties,» says Sabatini.

Biological and technical replicates for each population were hybridised to NIA Mouse 44K Microarray v2.3 (whole genome 60 mer oligonucleotide probe; manufactured by Agilent Technologies, # 014951)[36].

Whole - genome sequencing, chromosomal microarray analysis, and pharmacogenomics present new ethical questions and dilemmas for physicians to consider.

41) Chari R, Lockwood WW, Coe BP, Chu A, Macey D, Thomson A, Davies JJ, MacAulay C, Lam WL (2006) SIGMA: a system for integrative genomic microarray analysis of cancer genomes.

264/5: 45 Diagnostic utility of whole genome sequencing as an alternative to chromosomal microarray analysis in pediatric medicine.

Microarray profiling using the Affymetrix Mouse Genome 430 2.0 array, defines sets of genes that are specific to bone marrow - derived hematopoietic stem cells and their different immunological progeny.

We assessed the ability of a commercial DNA microarray to characterize bovine Shiga toxin - producing Escherichia coli (STEC) isolates and evaluated the results using in silico hybridization of the microarray probes within whole genome sequencing scaffolds.

113/4: 00 Characterization of mosaic chromothripsis in the human germline by chromosomal microarray and whole genome sequencing.

But when the researchers used DNA microarrays to survey the content of genes in each genome, they found significant differences among the strains.