Sentences with phrase «genome wide screens»

«We've now devised a technology where we can effectively treat the surface of living mouse embryos as a petri dish of cells, and carry out genome wide screens on mouse cells in their native environment in vivo,» says Fuchs.

In their latest study, the group conducted a genome wide screening by developing a novel primary mouse embryonic stem cell line that enhances the efficiency of CRISPR - Cas9 mediated mutations.

Not exact matches

Using a genome - wide genetic screen, Dr. Gelman and colleagues identified a previously unknown metastasis suppressor — the FOXO4 protein, which belongs to a family of genes that are produced by all human cells.

It is being used as a genome - wide tool, to search thousands of genes in a cell and screen for their function.

In addition, genome - wide screens indicate that the Del - 1 gene may contribute to multiple sclerosis risk.

By performing a genome - wide screen in breast cancer cells, Dr. Oesterreich and her colleagues identified a gene called HOXC10 as one that the cancer seems to modify to allow continued tumor growth in patients whose cancer becomes resistant to traditional therapies.

Researchers were able to complete the first genome - wide screen of a mouse by injecting small hairpin RNAs into the embryos of 100 pregnant mice and analyzing the animals after birth to look for genes that contributed to tumor growth.

They recently revealed the first genome - wide RNA interference screen of a mouse in an article published in Nature.

«Previously, genome - wide screens were only possible in lower animals, such as flies, worms and yeast, where it is often difficult to assess the relevance to human disorders such as cancer.»

Whitehead Institute researchers have conducted the first genome - wide screen in Apicomplexa, a phylum of single - celled parasites that cause malaria, babesiosis, cryptosporidiosis, and toxoplasmosis.

To understand how the HIPPO pathway controls Schwann cell proliferation and myelination, the researchers used genome - wide screens.

In collaboration with Dr. A. Gitler (Stanford University, USA), genome - wide genetic modifier screens were first performed in yeast.

«This is the next step in high - throughput, genome - wide screening,» Konermann said.

Such plants can be isolated using genome - wide association mapping of diverse populations or can be isolated from forward genetic screens, where a subset sample population with the desired traits is selected from a large pool of mutagenized individuals.

The perspectives are to upgrade the scale of the functional genomic screen to a genome wide study to get an highlight of all of the biological networks relevant to be targeted for DM1.

The discovery of short hairpin RNAs (shRNAs) suppressing gene expression in mammalian cells enables large - scale loss - of - function screens by using genome - wide shRNA libraries.

use CRISPR - Cas technology to carry out genome - wide screens of gene - gene, gene - drug and cancer - microenvironment interactions in cells and mice in order to explore fundamental biology and to identify drug targets and drug resistance / sensitisation mechanisms.

We have been studying endocytosis in a broader, systems perspective, by combining quantitative imaging, genome - wide RNAi screens with systems biology analysis.

Charles Bradshaw (Zerial, MPG)-- «Improving the analysis of genome - wide screens trough the detection of weakly conserved domains» (2009)

Our work using genome - wide RNAi screens has shown that rather small numbers of molecular components are required to organize each of these compartments.

We have undertaken a genome - wide functional screen on a mouse muscle cell line and identified hundreds of molecules implicated in the fusion of this cell line, with no effect on their proliferation or differentiation.

Mikolaj Slabicki (Buchholz, MPG)-- «A systematic investigation of DNA double strand break repair by a genome - wide RNAi screen in human cells» (2008)

All of the above concerns with Cas9 activators are, for the most part, less of an issue in the setting of large scale genome - wide screens as the only consequence of a gene not being properly up - regulated in this setting is a potentially missed hit rather than complete experimental failure.

Title: A genome - wide screen in EpiSCs identifies Nr5a nuclear receptors as potent inducers of ground state pluripotency Authors: Guo G, Smith A Date: October 2010 Publication Details: Development.

Improved vectors and genome - wide libraries for CRISPR screening.

Title: Inferring combinatorial association logic networks in multimodal genome - wide screens Authors: de Ridder J, Gerrits A, Bot J, de Haan G, Reinders M, Wessels L Date: June 2010 Publication Details: Bioinformatics.

Title: A genome - wide RNAi screen reveals MAP kinase phosphatases as key ERK pathway regulators during embryonic stem cell differentiation (IN PRESS, Oct 2012) Authors: Sharrocks A, Yang S - H, Kalkan T, Haslam C, Smith A Date: IN PRESS, Oct 2012 Publication Details: PLoS Genetics

The Cancer, Ageing and Somatic Mutation Programme seeks to provide leadership in data aggregation and informatics innovation, developing high - throughput cellular models of cancer for genome - wide functional screens and drug testing, and exploring basic scientific questions about the role somatic mutation plays in clonal evolution, ageing and development.

He is also interested in genes induced by the interferon response, and his future plans include investigating genes identified in the expression of genome - wide association screens for predisposition to cancers.

Many CRISPR plasmids are designed for lentiviral use, including Addgene's top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome - wide screening.

Researchers have been taking advantage of advances in computational biology and genome - wide screening techniques to identify genetic signatures for cancer prognosis.

lentiCas9 - Blast (Plasmid # 52962) was a gift from Feng Zhang and is described in Improved vectors and genome - wide libraries for CRISPR screening.

We have been developing a genome - wide methylation screening for the identification of new imprinted genes, and are investigating the mechanisms underlying imprinted regulation.

We compared the performance of three available second - generation human genome - wide CRISPR - KO libraries that included at least one of the improvements, and examined the effect of gRNA scaffold, number of gRNAs per gene and number of replicates on screen performance.

Here we show that loss of the BLM helicase complex suppresses FANCC phenotypes and we confirm this interaction in cells deficient for FA complementation group I and D2 (FANCI and FANCD2) that function as part of the FA I - D2 complex, indicating that this interaction is not limited to the FA core complex, hence demonstrating that systematic genome - wide screening approaches can be used to reveal genetic viable interactions for DNA repair defects.

Genome - wide CRISPR - based knockout (CRISPR - KO) screening is an emerging technique which enables systematic genetic analysis of a cellular or molecular phenotype in question.

Furthermore, a genome - wide forward screen with Haplobank identified PLA2G16 as a host factor that is required for cytotoxicity by rhinoviruses, which cause the common cold.

They also facilitate rapid deconvolution of signals from genome - wide screens.

Here, through a genome - wide CRISPR - Cas9 - mediated forward genetic screen, we identified MORC2 as an essential gene required for epigenetic silencing by the HUSH complex.

Project Title: In vivo genome - wide genetic screening for modifiers of mutant Huntingtin toxicity