Sentences with phrase «genomic loci»

Dynamic imaging of genomic loci in living human cells by an optimized CRISPR / Cas system.

We use genome - wide association of 87 Doberman Pinscher cases and 63 controls to identify genomic loci associated with OCD and sequence these regions in 8 affected dogs from high - risk breeds and 8 breed - matched controls.

These intracellularly expressed ssDNAs are targeted into specific genomic loci where they are recombined and converted into permanent memory.

Cas9 creates double - strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide.

For those who do not work with C. elegans, several groups have designed modular toolkits to assist with tagging genomic loci in other model systems, including mammalian cells.

«Unlike previous enhancer assays for mammals, SIF - seq includes the integration of putative enhancers into a single genomic locus,» says Visel.

Another is a modification of Cas9 called CRISPRainbow, a system that can more easily label multiple genomic loci in living cells, allowing researchers to better study chromatin dynamics.

Feralisation targets different genomic loci to domestication in the chicken.

Engineered cells are then validated via next - generation sequencing and analysis of the modified genomic loci with custom analytical tools developed by our bioinformatics team.

Furthermore, we are sequencing and assembling genomes of species selected for having interesting phenotypes in order to find genomic loci involved in these phenotypes.

To generate a reporter cell line that gives real time read outs of low - level early endodermal gene expression, we introduced a synthetic internal ribosomal entry site (IRES) designed to amplify translation upstream of a fluorescent reporter [31] into the first exon of the Hex genomic locus (Figure 1A).

Over 50 human genomic loci are associated with either pigmentation phenotypes or melanoma susceptibility.

Such genome packaging is not simply accomplished by condensing genetic material, but distant genomic loci are also non-randomly localized in the nucleus.

It is often useful to deliver distinct functions to separate genomic loci, such as, activation of a set of genes and repression of another set of genes.

, several groups have designed modular toolkits to assist with tagging genomic loci in other model systems, including mammalian cells.

The Cheng lab develops technologies based on artificial DNA and RNA binding proteins and applies them to sense or induce sequence or epigenetic changes at target genomic loci or target transcripts.

During the last several years, research efforts with human cell lines revealed that the genomic loci encoding tRNAs give rise to shorter tRNA fragments («tRFs»).

[Hana Lango Allen et al, Hundreds of variants clustered in genomic loci and biological pathways affect human height] Height has a lot to do with genetics.

In the presence of an input, ssDNAs (orange curved lines) are produced from a plasmid - borne cassette (gray circles) and recombined into specific genomic loci (orange circles) that are targeted on the basis of sequence homology.

To test this, we created translational reporter transgenes regulated by the putative endogenous promoter expressing isoform b and lines in which the genomic locus was tagged by an in - frame C - terminal GFP cassette.

All DruGeVar database records involve correlations between 91 drugs, 13 genomic loci and 98 genomic variants which has previously implicated in variable drug responses both in terms of efficacy and toxicity.

Hundreds of variants clustered in genomic loci and biological pathways affect human height.

Many, if not most of the genomic loci associated with human traits lie in noncoding regions.

Using a laser scanning confocal microscope, we have examined positions of the genomic loci in the 3D nuclear compartment at as short as 1.5 - second intervals.

Importantly, these studies to elucidate gene contacts in the 3D nuclei of live cells have demonstrated that condensin - mediated contacts between centromeres and the genomic loci carrying Pol III - transcribed genes or retrotransposons are highly dynamic.

These CRISPR inhibitors may provide a layer of regulation to genome engineering that enables new applications, such as reversing the effects of dCas9 binding to a genomic locus, or limiting the amount of time that Cas9 is active in the nucleus to reduce off - target gene editing.