Sentences with phrase «genomic sites»

Age - related variations in DNA methylation were analyzed by deep sequencing technology, which revealed distinct patterns, with gains in methylation in older individuals occurring at genomic sites that were unmethylated in young individuals, and vice versa.

The approach was also used to regulate gene transcription at endogenous genomic sites when combined with CRISPR / Cas9 technology.

Caribou Biosciences has generated hundreds of thousands of data points from engineering many thousands of genomic sites across several different cell types.

Users can design gRNAs to induce the break at a specific genomic site and, using the endogenous homology directed repair pathway, a new user - defined DNA sequence (like GFP) can be inserted at the DSB.

Scientists have long realized that as people age, their genomes undergo epigenetic changes: some genomic sites gain methyl groups, others lose them.

The power of this system lies in the ability of the endonuclease Cas9 to create a DNA double stranded break (DSB) at a genomic site specified by a guide RNA (gRNA) sequence.

These potential off - target sites were analyzed by DNA sequencing, but no unwanted mutation occurred at these genomic sites in all cloned goats (Table S2).