In brief, the studies consisted of seven analyses on gene regulation in undifferentiated ES cells, focusing on LIF targets, Stat3 and PI3K regulated genes, as well as
global gene expression changes through epigenetic mechanisms; and, four studies where ES cells were allowed to differentiate in monolayers or as embryoid bodies.
Li W, Turner A, Aggarwal P, Matter A, Storvick E, Arnett DK, Broeckel U. Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology
for global gene expression analysis.
As a Howard Hughes Medical Institute investigator and biochemistry professor at Stanford, Pat led a team that developed the first methods for analyzing, visualizing and
interpreting global gene expression programs.
Traditional strain - selection techniques are supplemented with strain screening, which can involve examining gene - expression patterns through PCR or
understanding global gene expression in relation to environmental factors using gene chips.
A further 25 days in medium containing FGF2 and Noggin mediated the derivation and expansion of RPCs which had a
similar global gene expression pattern to cells of the embryonic day 14 (E14) mouse retina.
Identifying the subcellular localization of huntingtin and understanding its effects
on global gene expression are critical to this endeavor.
Interestingly, a transcriptional analysis revealed that transgene expression from not completely silenced viral vectors could, in fact,
perturb global gene expression in hiPSCs [8].
Scientists at The Scripps Research Institute (TSRI) have identified a molecule in the brain that triggers schizophrenia - like behaviors, brain changes and
global gene expression in an animal model.
Using an experimental model of heart failure and next generation sequencing to get a snapshot of all of the RNA in the heart cells, the researchers identified
the global gene expression changes that occur in heart failure.
Further, his group pioneered expression quantitative trait locus (eQTL) studies, which enabled variation in
global gene expression to be applied to genetics of complex human diseases.
«Without computer analysis of sequencing results, we would not have been able to determine relationships between DNA methylation, how accessible regions of the genome were,
global gene expression, or the emergence of cancer cells,» says González - Avalos, a graduate student in UCSD's Bioinformatics Graduate Program.
Whitehead scientists identify major flaw in standard approach to
global gene expression analysis