Sentences with phrase «goat serum»

GFAP (1:5000 mouse monoclonal; Sigma - Aldrich G3893) or CD68 (1:500 rat monoclonal; AbD Serotec MCA1957) were diluted in 3 % normal goat serum with 0.2 % gelatin.
MuLV positive and negative goat serum controls are labelled.
Slides were rinsed in PBS three times (5 min each) and blocked in PBS containing 5 % normal horse serum and 1 % normal goat serum.
Samples were incubated with the primary antibody for 14 hours at 4ºC in PBS / 5 % goat serum.
The cells were then incubated in 1x PBS / 10 % normal goat serum / 0.3 M glycine to block non-specific protein - protein interactions followed by the antibody (ab9484, 1µg / 1x106 cells) for 30 min at 22ºC.
The cells were incubated at 37 °C for 3h in media containing different concentrations of ab120223 (FK506) in DMSO, fixed with 100 % methanol for 5 minutes at -20 °C and blocked with PBS containing 10 % goat serum, 0.3 M glycine, 1 % BSA and 0.1 % tween for 2h at room temperature.
The cells were fixed with 4 % formaldehyde (10 min) and then blocked in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h.
The cells were 100 % methanol fixed (5 min), permeabilized with 0.1 % Triton X-100 for 5 minutes and then incubated in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h to block non-specific protein - protein interactions.
Samples were incubated with primary antibody (1/100 in PBST with BSA and goat serum) for 4 °C at 12 hours.
The cells were fixed with 4 % formaldehyde (10 min), permeabilized with 0.1 % Triton X-100 for 5 minutes and then blocked in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h.
Sections were incubated in 0.3 % hydrogen peroxide in PBS for 20 min, washed in PBS - T (0.01 M phosphate - buffered saline, 0.2 % Triton X-100) and blocked 1 h with 10 % normal goat serum (NGS) in PBS - T.
The cells were fixed with 4 % formaldehyde (10 min), permeabilized in 0.1 % Triton X-100 for 5 minutes and then blocked in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h.
The cells were fixed with 100 % methanol (5 min) and then blocked in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h.
Cells were fixed with 100 % methanol (5 min) at room temperature and incubated with PBS containing 10 % goat serum, 0.3 M glycine, 1 % BSA and 0.1 % triton for 1h at room temperature to permeabilise the cells and block non-specific protein - protein interactions.
After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).
In addition, a separate set of goat sera was also tested in a blinded fashion and included external positive and negative controls consisting of dilutions of the MuLV whole virus, gp69 / 71 goat polyclonal antisera, or pre-immune goat sera, respectively.
Detection of antibody reactivity in the goat sera was done by using rabbit anti-goat HRP conjugate (Dako, Hamburg, Germany).
The cells were fixed with 100 % methanol for 5 minutes, permeabilized with 0.1 % Triton X-100 for 5 minutes and then blocked with 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1 hour.
The cells were 4 % PFA fixed (10 min) and then incubated in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h to permeabilise the cells and block non-specific protein - protein interactions.
The cells were then incubated in 1x PBS / 10 % normal goat serum / 0.3 M glycine to block non-specific protein - protein interactions followed by the antibody (ab51608, 1 / 11709 dilution) for 30 min at 22ºC.
The cells were incubated at 37 °C for 6h in media containing different concentrations of ab120723 (baicalein) in DMSO, fixed with 4 % formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10 % goat serum, 0.3 M glycine, 1 % BSA and 0.1 % tween for 2h at room temperature.
Samples were incubated with primary antibody (1/100 in 10 % goat serum) for 18 hours at 4 °C.
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