Sentences with phrase «granzyme a»

First, after culturing human peripheral blood mononuclear cells (hu - PBMCs) with the IL - 15 superagonist, flow cytometry revealed increases in granzyme B and perforin expression in the CD3 -, CD16 +, CD56 + NK cells.

In particular, they investigate how killer immune cells use pore forming protein perforin, antimicrobial peptide granulysin, and immune protease granzyme B in synergy to destroy pathogens in host cells.

To assess granzyme B production, CFSE - labeled cells were stimulated for 24 or 72 h, surface - stained as described above, permeabilized using a Cytofix / Cytoperm kit (BD Pharmingen), and then stained with flurochrome - labeled granzyme B Ab (GB11; Life Technologies) at 4 °C for 30 min prior to flow cytometric analyses.

Deletion of DGKζ or Cbl - b comparably enhanced CD8 + T cell functional responses, such as proliferation, production of IFN - γ, and generation of granzyme B when compared with wild type T cells.

(C) Intracellular granzyme B was measured from purified T cells gated from CD8 + populations after 24 and 72 h of stimulation with 0.3 μg / ml anti-CD3 stimulation.

Further, although we could detect enhancement of granzyme B production in DKO T cells relative to WT, DGKζ − / −, or Cbl - b − / − T cells at early time points (Fig. 3B, upper panels), we did not observe enhanced granzyme B production in DKO relative to DGKζ − / − or Cbl - b − / − T cells at later time points (Fig. 3B, lower panels), at higher or lower concentrations of anti-CD3 Ab, or with addition of anti-CD28 Ab (Supplemental Fig. 2B).

Consistent with previously reported analyses, we also observed enhanced IFN - γ and granzyme B production in DGKζ and Cbl - b — deficient T cells.

Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells.

They kill cells by releasing small cytoplasmic granules of proteins called perforin and granzyme that cause the target cell to die by apoptosis (programmed cell death).

Lieberman, Dotiwala and their team were particularly struck by how much the granzyme - sparked cascade resembles apoptosis, a controlled form of cellular suicide that helps eliminate damaged or potentially cancerous cells.

Lieberman notes that her laboratory reported a similar reaction to perforin, granulysin and granzyme in intracellular bacteria in a 2014 Cell paper.

They also found elevated granzyme A levels in blood samples taken from non-human primates infected with chikungunya, as well as from human chikungunya patients.

Exploring further, the scientists showed that mice missing the granzyme A gene, when infected with chikungunya virus, experienced dramatically less foot swelling and arthritis.

Overall the findings suggest that granzyme A could serve as a potential drug target for anti-inflammatory treatments for chikungunya — and perhaps also for other inflammatory diseases.

Not only do controllers have more CD8 T cells, each cell produces greater quantities of perforin and granzymes, which stimulate apoptosis, or cell death.

Particularly prominent in the RNA - Seq analysis was the up - regulation of a number of granzymes, a group of proteinases secreted by immune cells that were originally thought to be involved in killing (via apoptosis) virus infected cells or other target cells.

NK cells are cytotoxic; small granules in their cytoplasm contain special proteins such as perforin and proteases known as granzymes.

They found that granzymes interrupt metabolism within the parasite's mitochondria (the cellular power plant).