Sentences with phrase «guide sequences of»

Her team's full Argonaute structure, published in 2005, definitively solved a key part of the mystery: the protein features a groove into which tiny guide sequences of RNA can nestle.

This section consists of paper - pencil «tools» used to guide the sequence of tasks followed in Science IDEAS instructional planning.

Not exact matches

It would «be impossible in practise [sic] for MS to revoke every bootmgr» — the code that guides the earliest stages of a computer's startup sequence — Slipstream wrote, «as they'd break install media, recovery partitions, backups, etc..»

Here's how it works: When a bacterium encounters DNA from a virus, it makes a strand of RNA, a molecular cousin to DNA, that matches the sequence of the viral DNA, known as a guide RNA.

Using sequences of connected content and CTAs, you can increase your marketing impact at each stage of the buyer's funnel and guide audiences over time from one step to the next.

If left undisturbed after birth, in skin - to - skin contact with the mother, the newborn — guided especially by her sense of smell and using her innate primitive reflexes - goes through a predictable sequence of behaviors during which:

Our Family Yoga Slow Flow class guides you through a sequence of gentle slow - flowing poses designed to help relieve stress and tension in the body.

Ruby's Studio: The Feelings Show: Adorable host Ruby guides kids on a magical journey of learning through a feelings themed art project, original music videos, gorgeous animated sequences, and puppetry.

The object is to be the first player to get rid of all the cards in your hand by placing them into color sequences based on the order of colors on the Spectrix guide card or into collections of the same color.

It consists of a pair of molecular scissors for cutting a DNA strand, and a guide part that binds to a specific DNA sequence.

, Draft 21, Weather, Draft 23, (Comparing Volumes), 17 (Observing Change), and 38 (Using Graphs), Drafts 22, 24, 28, 33, 35 & 50, Drafts 41, 42 & 48, Drafts 1, Draft 2 - 4, «Finished» 4 - 6, Drafts 8, 11 & 12, Drafts 13 & 14, Drafts 16, 18 & 34, Drafts SAPA Modules 16, 18 & 34, Drafts, 1973 Reports, Experimental Module SAPA, 1973 - 1974 SAPA II Program Guide Revision, 1973 Supplementary Study Materials Report, «Big M Game» and «Booklet Averages», 1973 - 1974 SAPA Alternate Organizational Schemes, 1973 Misc., including correspondences, addresses, lists of modules and material lists, 1973 - 1973; 1975 Program Guide, drafts and suggestions Program Chart, 1973 Description of the Program, Drafts, 1973 Mars Photos, 1973 Sequence Charts, Modules 16 - 30, 1973 Sequence Charts Part A, Modules 1 - 15, 1973 Long - Range Planning Guide - Capie Clusters Sequence Charts, Modules 31 - 45, 1975 Sequence Charts, Modules 31 - 60, 1973 Sequence Charts, Modules 46 - 60.

One route, first suggested by Burt in 2003, is to release a sequence that is resistant — effectively unrecognizable — to the guiding enzyme that finds cuts of DNA in a gene drive.

By delivering this version of Cas9 along with the guide RNA strand into single cells, the researchers can target one genetic sequence per cell.

CRISPR is a combination of an enzyme that can cut a selected DNA sequence and a «guide RNA» that takes the enzyme exactly where you want to make the cut — in this case, at the sickle cell mutation.

The two chambers appear to be connected, which could help explain the sequence of events in the 1980 eruption and guide volcanologists monitoring the mountain for signs of renewed activity.

Lu's team will extract immune cells called T cells from the blood of the enrolled patients, and then use CRISPR — Cas9 technology — which pairs a molecular guide able to identify specific genetic sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the cells.

Solving this particular mystery shows the potential value of using environmental sequencing to guide taxonomic and ecological discovery.»

To do this, CRISPR searches the organism's DNA for a certain sequence — specifically, the one encoded by the guide RNA, which holds the inverse sequence of your target DNA.

To preserve genetic diversity and guide breeding, geneticist Kathy Belov is sequencing portions of the DNA of every captive devil — an unprecedented level of sophistication for any captive breeding program.

By deleting some of these «ultraconserved elements», researchers have found that these sequences guide brain development by fine - tuning the expression of protein - coding genes.

This RNA snippet, known as sgRNA (single guide RNA), contains a sequence of 20 RNA - letters complementary to the genomic target site that scientists have hitherto had to select laboriously by hand or with a variety of online tools.

One of these proteins, a DNA - cutting enzyme called Cas9, binds to short RNA guide strands that target specific sequences, telling Cas9 where to make its cuts.

Any RNA, when in a complex with another oligoribonucleotide known as an external guide sequence (EGS), can become a substrate for ribonuclease P. Simulation of evolution in vitro was used to select EGSs that bind tightly to a target substrate messenger RNA and that increase the efficiency of cleavage of the target by human ribonuclease P to a level equal to that achieved with natural substrates.

Although the Arabidopsis sequence can itself serve as a guide to other plant genomes, plans are underway to sequence the rice genome and researchers are debating the merits of sequencing maize and other crops.

The barley genome sequence also enabled the identification of regions of the genome that have been vulnerable to genetic bottlenecking during domestication, knowledge that helps to guide breeders to optimize genetic diversity in their crop improvement efforts.

In this new study, we show that, just as these folding maps trace the contour of the genome as it folds inside the nucleus, they can also guide us through the sequence itself.»

The pictures show that human Argonaute has a single binding site for GW182, and that binding of the guide sequence increases the affinity of the Argonaute and GW182.

But that is only a rough guide, and in recent years geneticists have been filling in the details of that picture by sequencing the DNA of both modern and ancient populations.

While circulating tumor DNA tests targeting a smaller set of cancer genes are already available for use in routine practice to guide care, by covering a much larger number of cancer genes, this high - intensity sequencing approach may enable development of future tests for early detection of cancer.

«Nearly every animal has these small RNAs, and they use them as a guide to look for target sequences and silence them,» said Heng - Chi Lee, PhD, assistant professor of molecular genetics and cell biology at UChicago and senior author of the new study.

Guide RNA recognizes the DNA sequence of target genome and the deaminase modifies the base of the unwound DNA.

By focusing on several of these mutations and screening through guide RNA variants with different mismatch combinations, they were able to identify specific guide RNAs that stimulated Cas9 activity towards the mutated gene sequences but left the normal counterpart untouched.

Adaptive immunity is conferred by the integration of DNA sequences from an invading element into the CRISPR array (adaptation), which is transcribed into long pre-CRISPR RNAs (pre-crRNAs) and processed into short crRNAs (expression), which guide Cas proteins to specifically degrade the cognate DNA on subsequent exposures (interference).

Through the combination of CRISPRs and RNA - guided endonucleases, such as Cas9, («Cas» stands for CRISPR - associated), bacteria are able to utilize small customized crRNA molecules (for CRISPR RNA) to guide the targeting and degradation of matching DNA sequences in invading viruses and plasmids to prevent them from replicating.

«We hypothesized that for a given pair of targets that differ by a single point mutation, a set of mismatches could be identified in the guide RNA that would eliminate Cas9's activity on the normal sequence while maintaining robust activity on the one with a deleterious point mutation.

CRISPR, originally discovered by biologists studying the bacterial immune system, consists of a DNA - cutting enzyme called Cas9 and short RNA guide strands that target specific sequences of the genome, telling Cas9 where to make its cuts.

«Our results reveal two major functions of the PAM that explain why it is so critical to the ability of Cas9 to target and cleave DNA sequences matching the guide RNA,» says Jennifer Doudna, the biochemist who led this study.

The sequence neatly encapsulates the guiding philosophy of the Aztecs: Life thrives only by the grace of the dead.

That money, most of it from federal grants through the Human Genome Project, has paid for the first rough guides to the 3 billion nucleotides in human DNA: maps studded with thousands of landmarks called «sequence tagged sites» (Science, 25 October 1996, p. 540).

Jennifer Doudna and Samuel Sternberg used a combination of single - molecule imaging and bulk biochemical experiments to show how the RNA - guided Cas9 enzyme is able to locate specific 20 - base - pair target sequences within genomes that are millions to billions of base pairs long.

Sullivan ordered the countdown for lethal force, and his pilot began the 10 - minute sequence for launch of a laser - guided Hellfire missile.

g (acceleration due to gravity) G (gravitational constant) G star G1.9 +0.3 gabbro Gabor, Dennis (1900 — 1979) Gabriel's Horn Gacrux (Gamma Crucis) gadolinium Gagarin, Yuri Alexeyevich (1934 — 1968) Gagarin Cosmonaut Training Center GAIA Gaia Hypothesis galactic anticenter galactic bulge galactic center Galactic Club galactic coordinates galactic disk galactic empire galactic equator galactic habitable zone galactic halo galactic magnetic field galactic noise galactic plane galactic rotation galactose Galatea GALAXIES galaxy galaxy cannibalism galaxy classification galaxy formation galaxy interaction galaxy merger Galaxy, The Galaxy satellite series Gale Crater Galen (c. AD 129 — c. 216) galena GALEX (Galaxy Evolution Explorer) Galilean satellites Galilean telescope Galileo (Galilei, Galileo)(1564 — 1642) Galileo (spacecraft) Galileo Europa Mission (GEM) Galileo satellite navigation system gall gall bladder Galle, Johann Gottfried (1812 — 1910) gallic acid gallium gallon gallstone Galois, Évariste (1811 — 1832) Galois theory Galton, Francis (1822 — 1911) Galvani, Luigi (1737 — 1798) galvanizing galvanometer game game theory GAMES AND PUZZLES gamete gametophyte Gamma (Soviet orbiting telescope) Gamma Cassiopeiae Gamma Cassiopeiae star gamma function gamma globulin gamma rays Gamma Velorum gamma - ray burst gamma - ray satellites Gamow, George (1904 — 1968) ganglion gangrene Ganswindt, Hermann (1856 — 1934) Ganymede «garbage theory», of the origin of life Gardner, Martin (1914 — 2010) Garneau, Marc (1949 ---RRB- garnet Garnet Star (Mu Cephei) Garnet Star Nebula (IC 1396) garnierite Garriott, Owen K. (1930 ---RRB- Garuda gas gas chromatography gas constant gas giant gas laws gas - bounded nebula gaseous nebula gaseous propellant gaseous - propellant rocket engine gasoline Gaspra (minor planet 951) Gassendi, Pierre (1592 — 1655) gastric juice gastrin gastrocnemius gastroenteritis gastrointestinal tract gastropod gastrulation Gatewood, George D. (1940 ---RRB- Gauer - Henry reflex gauge boson gauge theory gauss (unit) Gauss, Carl Friedrich (1777 — 1855) Gaussian distribution Gay - Lussac, Joseph Louis (1778 — 1850) GCOM (Global Change Observing Mission) Geber (c. 720 — 815) gegenschein Geiger, Hans Wilhelm (1882 — 1945) Geiger - Müller counter Giessler tube gel gelatin Gelfond's theorem Gell - Mann, Murray (1929 ---RRB- GEM «gemination,» of martian canals Geminga Gemini (constellation) Gemini Observatory Gemini Project Gemini - Titan II gemstone gene gene expression gene mapping gene pool gene therapy gene transfer General Catalogue of Variable Stars (GCVS) general precession general theory of relativity generation ship generator Genesis (inflatable orbiting module) Genesis (sample return probe) genetic code genetic counseling genetic disorder genetic drift genetic engineering genetic marker genetic material genetic pool genetic recombination genetics GENETICS AND HEREDITY Geneva Extrasolar Planet Search Program genome genome, interstellar transmission of genotype gentian violet genus geoboard geode geodesic geodesy geodesy satellites geodetic precession Geographos (minor planet 1620) geography GEOGRAPHY Geo - IK geologic time geology GEOLOGY AND PLANETARY SCIENCE geomagnetic field geomagnetic storm geometric mean geometric sequence geometry GEOMETRY geometry puzzles geophysics GEOS (Geodetic Earth Orbiting Satellite) Geosat geostationary orbit geosynchronous orbit geosynchronous / geostationary transfer orbit (GTO) geosyncline Geotail (satellite) geotropism germ germ cells Germain, Sophie (1776 — 1831) German Rocket Society germanium germination Gesner, Konrad von (1516 — 1565) gestation Get Off the Earth puzzle Gettier problem geyser g - force GFO (Geosat Follow - On) GFZ - 1 (GeoForschungsZentrum) ghost crater Ghost Head Nebula (NGC 2080) ghost image Ghost of Jupiter (NGC 3242) Giacconi, Riccardo (1931 ---RRB- Giacobini - Zinner, Comet (Comet 21P /) Giaever, Ivar (1929 ---RRB- giant branch Giant Magellan Telescope giant molecular cloud giant planet giant star Giant's Causeway Giauque, William Francis (1895 — 1982) gibberellins Gibbs, Josiah Willard (1839 — 1903) Gibbs free energy Gibson, Edward G. (1936 ---RRB- Gilbert, William (1544 — 1603) gilbert (unit) Gilbreath's conjecture gilding gill gill (unit) Gilruth, Robert R. (1913 — 2000) gilsonite gimbal Ginga ginkgo Giotto (ESA Halley probe) GIRD (Gruppa Isutcheniya Reaktivnovo Dvisheniya) girder glacial drift glacial groove glacier gland Glaser, Donald Arthur (1926 — 2013) Glashow, Sheldon (1932 ---RRB- glass GLAST (Gamma - ray Large Area Space Telescope) Glauber, Johann Rudolf (1607 — 1670) glaucoma glauconite Glenn, John Herschel, Jr. (1921 ---RRB- Glenn Research Center Glennan, T (homas) Keith (1905 — 1995) glenoid cavity glia glial cell glider Gliese 229B Gliese 581 Gliese 67 (HD 10307, HIP 7918) Gliese 710 (HD 168442, HIP 89825) Gliese 86 Gliese 876 Gliese Catalogue glioma glissette glitch Global Astrometric Interferometer for Astrophysics (GAIA) Global Oscillation Network Group (GONG) Globalstar globe Globigerina globular cluster globular proteins globule globulin globus pallidus GLOMR (Global Low Orbiting Message Relay) GLONASS (Global Navigation Satellite System) glossopharyngeal nerve Gloster E. 28/39 glottis glow - worm glucagon glucocorticoid glucose glucoside gluon Glushko, Valentin Petrovitch (1908 — 1989) glutamic acid glutamine gluten gluteus maximus glycerol glycine glycogen glycol glycolysis glycoprotein glycosidic bond glycosuria glyoxysome GMS (Geosynchronous Meteorological Satellite) GMT (Greenwich Mean Time) Gnathostomata gneiss Go Go, No - go goblet cell GOCE (Gravity field and steady - state Ocean Circulation Explorer) God Goddard, Robert Hutchings (1882 — 1945) Goddard Institute for Space Studies Goddard Space Flight Center Gödel, Kurt (1906 — 1978) Gödel universe Godwin, Francis (1562 — 1633) GOES (Geostationary Operational Environmental Satellite) goethite goiter gold Gold, Thomas (1920 — 2004) Goldbach conjecture golden ratio (phi) Goldin, Daniel Saul (1940 ---RRB- gold - leaf electroscope Goldstone Tracking Facility Golgi, Camillo (1844 — 1926) Golgi apparatus Golomb, Solomon W. (1932 — 2016) golygon GOMS (Geostationary Operational Meteorological Satellite) gonad gonadotrophin - releasing hormone gonadotrophins Gondwanaland Gonets goniatite goniometer gonorrhea Goodricke, John (1764 — 1786) googol Gordian Knot Gordon, Richard Francis, Jr. (1929 — 2017) Gore, John Ellard (1845 — 1910) gorge gorilla Gorizont Gott loop Goudsmit, Samuel Abraham (1902 — 1978) Gould, Benjamin Apthorp (1824 — 1896) Gould, Stephen Jay (1941 — 2002) Gould Belt gout governor GPS (Global Positioning System) Graaf, Regnier de (1641 — 1673) Graafian follicle GRAB graben GRACE (Gravity Recovery and Climate Experiment) graceful graph gradient Graham, Ronald (1935 ---RRB- Graham, Thomas (1805 — 1869) Graham's law of diffusion Graham's number GRAIL (Gravity Recovery and Interior Laboratory) grain (cereal) grain (unit) gram gram - atom Gramme, Zénobe Théophile (1826 — 1901) gramophone Gram's stain Gran Telescopio Canarias (GTC) Granat Grand Tour grand unified theory (GUT) Grandfather Paradox Granit, Ragnar Arthur (1900 — 1991) granite granulation granule granulocyte graph graph theory graphene graphite GRAPHS AND GRAPH THEORY graptolite grass grassland gravel graveyard orbit gravimeter gravimetric analysis Gravitational Biology Facility gravitational collapse gravitational constant (G) gravitational instability gravitational lens gravitational life gravitational lock gravitational microlensing GRAVITATIONAL PHYSICS gravitational slingshot effect gravitational waves graviton gravity gravity gradient gravity gradient stabilization Gravity Probe A Gravity Probe B gravity - assist gray (Gy) gray goo gray matter grazing - incidence telescope Great Annihilator Great Attractor great circle Great Comets Great Hercules Cluster (M13, NGC 6205) Great Monad Great Observatories Great Red Spot Great Rift (in Milky Way) Great Rift Valley Great Square of Pegasus Great Wall greater omentum greatest elongation Green, George (1793 — 1841) Green, Nathaniel E. Green, Thomas Hill (1836 — 1882) green algae Green Bank Green Bank conference (1961) Green Bank Telescope green flash greenhouse effect greenhouse gases Green's theorem Greg, Percy (1836 — 1889) Gregorian calendar Grelling's paradox Griffith, George (1857 — 1906) Griffith Observatory Grignard, François Auguste Victor (1871 — 1935) Grignard reagent grike Grimaldi, Francesco Maria (1618 — 1663) Grissom, Virgil (1926 — 1967) grit gritstone Groom Lake Groombridge 34 Groombridge Catalogue gross ground, electrical ground state ground - track group group theory GROUPS AND GROUP THEORY growing season growth growth hormone growth hormone - releasing hormone growth plate Grudge, Project Gruithuisen, Franz von Paula (1774 — 1852) Grus (constellation) Grus Quartet (NGC 7552, NGC 7582, NGC 7590, and NGC 7599) GSLV (Geosynchronous Satellite Launch Vehicle) g - suit G - type asteroid Guericke, Otto von (1602 — 1686) guanine Guiana Space Centre guidance, inertial Guide Star Catalog (GSC) guided missile guided missiles, postwar development Guillaume, Charles Édouard (1861 — 1938) Gulf Stream (ocean current) Gulfstream (jet plane) Gullstrand, Allvar (1862 — 1930) gum Gum Nebula gun metal gunpowder Gurwin Gusev Crater gut Gutenberg, Johann (c. 1400 — 1468) Guy, Richard Kenneth (1916 ---RRB- guyot Guzman Prize gymnosperm gynecology gynoecium gypsum gyrocompass gyrofrequency gyropilot gyroscope gyrostabilizer Gyulbudagian's Nebula (HH215)

The Quick Guide includes descriptions of these organisms and has links to sequencing centers and scientific abstracts.

We should move quickly to collect the information necessary for well - guided decision making, because one thing is clear: Next - gen sequencing will soon be a routine part of clinical care, whether we like it or not.

The advancement of the Cas9 - based platform for screening and validation will help further the development of new therapeutic products, and Caribou's CRISPR - Cas9 technology can utilize guide RNAs specific for unique sequences and target a gene at numerous sites and therefore provide enhanced specificity.

In this case, the pattern is the main sequence, which has proven to be a valuable guide for theoreticians as they have developed conceptual and mathematical models of stellar evolution.

Cas9 can be programmed to use guide RNAs to target the protein to a specific sequence within double - stranded DNA, enabling simple, flexible targeting of nearly any site in a given genome.

In their zebrafish model, the barcode contains 10 20 - base - pair sequences targeted by Cas9 endonuclease, with each of the sequences matching a specific single guide RNA.

Guided Protocol for Fecal Microbial Characterization by 16S rRNA - Amplicon Sequencing — Ayelet Di Segni — Journal Of Visualized Experiments (It includes a video)

The genomic CD81 region of each clone was PCR amplified and sequenced using the Guide - it Indel Identification Kit to obtain detailed sequence information.