For isolation of nuclei, embryos were collected overnight (0 — 16
h after egg laying [AEL]-RRB- onto apple juice agar plates and dechorionated in 25 % bleach for 5 min.
This video shows ventral and lateral maximum - intensity projections of the SiMView time - lapse recording of the nuclei - labeled Drosophila embryo, superimposed with green spheres marking the location, movements and divisions of neural precursors from the blastoderm stage up to 5
h after egg laying.
The tracks are represented by solid lines, using a color code that indicates time (purple to white: from the blastoderm stage to the end point of the reconstruction at 5
h after egg laying).
Not exact matches
One day
after mating,
eggs were transferred to a Petri dish, where they were treated with lye diluted to 0.5 % in water for 90 s. Then, they were washed 3 times for 3 min with water, and maintained for 24
h at 28.5 °C.