The visible hydrate was found as thin icelike layers that released methane gas initially upon retrieval, but stabilized for up to 4
h at atmospheric pressure conditions and subfreezing temperatures.
With this labeling method, the intrinsically labeled Golden Rice β - carotene showed a protonated molecule of m / z of Mβc +
H + = 536 + 1 (representing unlabeled β - carotene) and a range of isotopomers with the most abundant showing an enrichment of 9 deuterium
at Menrich - βc = Mβc +
H + + 9 mass units (Figure 4) as analyzed by using the liquid chromatography / mass spectrometry with a positive
atmospheric pressure chemical ionization interface (the total m / z was Mβc + 9 +
H + = 536 + 9 + 1 = m / z 546)(23).