Sentences with phrase «h embryo»

For hatching assays, 0 - to 16 - h embryos were collected on apple juice agar plates and allowed to develop for an additional 25 h at 25 °C.

Not exact matches

Scientists from the Institute of Molecular Biotechnology (IMBA) in Vienna, Austria, have discovered how an embryo's genomic integrity is safeguarded during the first 24 h after fertilization.

This video shows ventral and lateral maximum - intensity projections of the SiMView time - lapse recording of the nuclei - labeled Drosophila embryo, superimposed with green spheres marking the location, movements and divisions of neural precursors from the blastoderm stage up to 5 h after egg laying.

(G — L) In situ hybridization of wild - type embryos injected with wild - type (G — I) or mutant ACVR1 (J — L) to detect ventral markers eve1 (G, n = 12/12; J, n = 10/13; onset of gastrulation) and gata2 (H, n = 14/14; K, n = 15/19; mid-gastrulation) or dorsal marker foxb1.2 (I, n = 12/12; L, n = 6/13, 7/13 showed no expression; mid-gastrulation).

The growth potential of excised embryos cultured on 1/2 MS medium declined by 31 % after 1 month of dry storage or after 24 h of dry storage at − 18 °C (Table 3), but growth was similar to that of embryos from freshly collected seeds (c. 80 %) when embryos were cultured with growth hormones.

Embryos were extracted and cultured from freshly collected seeds, seeds that had been stored dry at 15 °C and 15 % RH for 1, 3 or 12 months and seeds that had been stored at − 18 °C for 24 h or 3 months after drying to stable moisture content (5 %, determined gravimetrically) for 2 weeks at 15 °C and 15 % RH.

Embryo cultures were maintained at 22 — 24 °C for 8 weeks, in darkness for the first 2 weeks followed by a 16 - h / 8 - h light — dark cycle.

(H) The lipid containing structures progressively diminish in size in mutant embryos during later stages of embryonic development (arrowhead).

Fused reconstructed embryos were further activated in 5 µM ionomycin for 5 min, followed by exposure to 1.9 mM 6 - dimethylaminopurine (DMAP) in synthetic oviduct fluid with amino acids (SOFaa) for 4 h. Following activation, embryos were then transferred and cultured in SOFaa.

For LacZ expression analysis, embryos were collected 2 — 8 h AEL and dechorionated in 25 % bleach for 3 min.

For isolation of nuclei, embryos were collected overnight (0 — 16 h after egg laying [AEL]-RRB- onto apple juice agar plates and dechorionated in 25 % bleach for 5 min.

Gardner DK, Rodriegez - Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer.

Differences in intracellular pH regulation by Na + / H + antiporter among two - cell mouse embryos derived from females of different strains.