Sentences with phrase «h incubation»

To test whether drought stress affected cellular integrity, REL was determined after 24 h incubation of leaf disks in water (Figure 2B).

The sections were then washed and incubated in the appropriate biotinylated secondary antibodies (1:1000 Vector Laboratories) for 1 h, followed by a 1 h incubation with avidin - biotin complex (Vectastain Elite, Vector Laboratories).

After an additional 24 h incubation at 28.5 °C, effects on blood vessels were observed with a binocular lens with filters for fluorescence and photographs were taken from relevant images.

To obtain brains, w1118 larvae staged at the onset of the L3 were fed on normal food for 12 h followed by 12 h incubation on 1 % non-nutritive agar to induce strong ILP accumulation.

In fact, at least 5.6 d would be needed for an infectious agent with only a 2 - h incubation time and with the current city populations in the Greater Tokyo metropolitan area to cover the distance between any two cities using only secondary infections, even if there were up to 1 % asymptomatic individuals in the population (Fig. 4).

After a 5 - h incubation period, the cells were detached from the flask with a 0.25 % trypsin - 0.02 % EDTA solution (volume for volume).

Not exact matches

Phase - contrast images of organ - derived endothelial cells (37 °C) on Matrigel surface (A) and after 4 h of incubation with DiI - Ac - LDL (B).

Endothelial cells derived from the brain demonstrated a complete absence of uptake after 4 h of incubation (at 33 °C or 37 °C) with this fluorescent reagent (data not shown).

Medium was replaced after incubation for 12 h at 37 °C.

The calcification rate values (µmol CaCO3 h − 1 cm − 2) were calculated according to the equation: Δ A T 2 ∗ V chamber − V coral T ∗ A coral where ΔAT is the difference in Total Alkalinity (AT) measured between the beginning and the end the incubation period, V is the volume of the chamber or the coral fragment, T is the duration of the incubation and A is the coral surface area.

The membrane was blocked with 3 % BSA / PBS for 1 h at RT followed by incubation with the corresponding primary antibody in 1 % BSA / PBS - T overnight at 4 °C: pTyr (Millipore, # 05 - 321, 1:500), Tie2 (R&D, #AF313, 1:1,000), Tie2 (R&D, #AF762), pAKT (Cell Signaling, # 4060S, 1:1,000), AKT (Cell Signaling, # 9272S, 1:1,000), FOXO1 (Cell Signaling, # 9454, 1:1,000), pFOXO3A (Cell Signaling, # 9466, 1:1,000), FOXO3A (Cell Signaling, # 12829, 1:1,000), VEGFR2 (Cell Signaling, # 2479, 1:1,000), tubulin (Sigma, #T8203, 1:5,000).

After 1 h and 10 min of incubation at 37 °C, tissue was broken using first a 19G followed by a 21G needle.

After 24 h of incubation, media was aspirated and replaced with media containing 20 µM concentrations of U0126, LY294002 or without drugs.

After 24 h of incubation, cells in the flask were treated with 1.0, 2.0 and 3.0 Gy electron beam radiation (Microtron Centre, Mangalore University, India).

After overnight culture, they were treated with a range of concentrations of ActD for 24 and 48 h, followed by incubation with methylthiazolyldiphenyl - tetrazolium bromide (MTT)(Sigma) for the assay.

A total of 50 μl of Protein A-agarose (Santa Cruz Biotechnology) was then added into each sample and incubation continued for 2 h at 4 °C.

The membranes were incubated with mouse sera obtained 3 - 5 weeks after inoculation (1:200 dilution), followed by three wash steps for 15 min each and incubation for 1 - 2 h with peroxidase - conjugated sheep anti-mouse IgG (whole molecule) or goat anti-mouse polyvalent immunoglobulins (1:10,000 dilution)(Sigma - Aldrich).

Protein G beads was then added and incubation continued for 1.5 h at 4 °C.

RESULTS: In L6 muscle cells, short - term incubations (2 - 12 h) with 10 -LRB--3) m alpha - lipoic acid increased glucose uptake by 40 - 80 %, approximately the same extent as 10 -LRB--6) m insulin.

Tyrosine was determined in the incubation medium by fluorescence (14), and protein breakdown was expressed as nmol Tyr / (mg protein · h).

Bracho R, Natali S, Pegoraro E, Crummer KG, Schädel C, Celis G, Hale L, Wu L, Yin H, Tiedje JM, Konstantinidis KT, Luo Y, Zhou J, Schuur EAG (2016) Temperature sensitivity of organic matter decomposition of permafrost - region soils during laboratory incubations.