For (D)--(F), 5 × 104 Stemcell Technologies — purified naive CD8 + T
cells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72 h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cells
cells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72
h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cells
Cells were surface - stained for
viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated
cellscells (D).