Sentences with phrase «hematopoietic differentiation»

The cell clusters were dismantled and subsequently treated with a hematopoietic differentiation medium.

Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

Finally, I will show how we have combined our results to generate a model of hematopoietic differentiation where specific transcription factors control lineage regulatory regions; our model predicts many already known lineage - controlling factors as well as finds new potential regulators of hematopoietic differentiation such as ATF3 in monocytes and Tcf7l2 and Runx2 in NK cells.

Not exact matches

«In normal conditions, the microenvironment is able to control the proliferation, differentiation and migration of the hematopoietic stem cell.

We examine their original thymic development: selection on hematopoietic cells leading to the acquisition of an original differentiation program.

While the hematopoietic system is perhaps the most characterized model system of cellular commitment and differentiation, we...

Expression of the melanoma cell adhesion molecule in human mesenchymal stromal cells regulates proliferation, differentiation, and maintenance of hematopoietic stem and progenitor cells.

MSC interact with hematopoietic stem cells (HSC), influencing their homing and differentiation through cell - cell contact and the production of factors and chemokines.

Hematopoiesis, where a single hematopoietic stem cell gives rise to the entire blood system, is an important model for differentiation with great medical importance.

Before coming to Columbia she worked as a technician in Leonard Zon's lab at Harvard Medical School using zebrafish as a model for investigating the molecular mechanisms of hematopoietic stem cell differentiation and self renewal.

Title: The combination of valproic acid and lithium delays hematopoietic stem / progenitor cell differentiation Authors: Walasek MA, Bystrykh LV, van den Boom V, Olthof S, Ausema A, Ritsema M, Huls G, de Haan G and van Os R Date: 2012 Publication Details: Blood 2012 Mar 29; 119 (13): 3050 - 59

Professor Kyurkchiev's group is currently developing a project on the controlled differentiation of human induced pluripotent stem cells into hematopoietic stem cells and lymphoid cells.

He has been interested in differentiation and growth control throughout his career and is an expert on hematopoietic stem cells, macrophage differentiation, hematopoietic and Maf family transcription factors.

This is in accordance with previous reports that decitabine and 5 - azacytidine produce a marked synergistic effect in combination with suberoylanilide hydroxamic acid and romidepsin in T - lymphoma cell lines by modulating cell cycle arrest and apoptosis.26, 27 As a mechanism of action, KMT2D mutations of B - lymphoma cells promote malignant outgrowth by perturbing methylation of H3K4 that affect the JAK - STAT, Toll - like receptor, or B - cell receptor pathway.28, 29 Here our study indicated that dual treatment with chidamide and decitabine enhanced the interaction of KMT2D with the transcription factor PU.1, thereby inactivating the H3K4me - associated signaling pathway MAPK, which is constitutively activated in T - cell lymphoma.13, 30,31 The transcription factor PU.1 is involved in the development of all hematopoietic lineages32 and regulates lymphoid cell growth and transformation.33 Aberrant PU.1 expression promotes acute myeloid leukemia and is related to the pathogenesis of multiple myeloma via the MAPK pathway.34, 35 On the other hand, PU.1 is also shown to interact with chromatin remodeler and DNA methyltransferease to control hematopoiesis and suppress leukemia.36 Our data thus suggested that the combined action of chidamide and decitabine may interfere with the differentiation and / or viability of PTCL - NOS through a PU.1 - dependent gene expression program.

RIG - G gene expression can be induced not only by ATRA along with the differentiation of NB4 cells but also by IFNα in a series of hematopoietic cell lines as well as various types of solid carcinoma cells (1, 3), which pointed to a possible role of RIG - G in signal cross-talk between ATRA and IFNα.

Moreover, ES cell differentiation in vitro recapitulates events that take place during early embryonic development including the formation of the three germ layers of ectoderm, mesoderm and endoderm, and the emergence of endothelial, hematopoietic, cardiac, neuronal and hepatic or pancreatic cells [8], [9].

Here, ES cells transduced with HOXB4 were analyzed for the expression of hematopoietic markers after 26 days of differentiation.

IL - 6 has been shown to be involved in diverse physiological processes such as T - cell activation, induction of immunoglobulin secretion, initiation of hepatic acute phase protein synthesis, and stimulation of hematopoietic precursor cell proliferation and differentiation.