Importantly, these cells expressed CD45, with smaller subpopulations expressing the important
hematopoietic markers CD117 and Sca - 1, suggesting that these cells may have the potential to populate bone marrow and eventually replenish senescent peripheral blood hematopoietic cells.
Here, ES cells transduced with HOXB4 were analyzed for the expression of
hematopoietic markers after 26 days of differentiation.
MSC do not express
hematopoietic markers, but a specific pattern of molecules, such as SH2 (CD105), SH3, SH4 (CD73) and STRO - 1.
Not exact matches
When Busch turned on the
marker in adult animals, it became visible that at least one third (approximately 5000 cells) of a mouse's
hematopoietic stem cells produce differentiated progenitor cells.
Nearly 30 years after the discovery of the
hematopoietic stem cell, Stanford researchers have found a
marker that allows them to study the version of these stem cells that continues to replicate.
There are no specific
markers to identify them; however, they are negative for
hematopoietic cell
markers like CD34 / 45 / HLA - DR and express CD90 / 73/105 on their surface 2, 3, 4.
Hematopoietic malignancies were diagnosed using the following
markers: for B - and T - lymphocyte detection, rat antimouse CD45R / B220 mAb (Southern Biotechnology Associates, Birmingham, AL; dilution 1:100); for T - lymphocyte detection, rabbit antihuman CD3 polyclonal antibody (Dako, Glostrup, Denmark; 1:100); and for monocyte / macrophage detection, rat antimouse F4 / 80 mAb (BMA Biomedicals AG, Augst, Switzerland; 1:20) and rat antimouse CD11b mAb (Chemicon International, Temecula, CA; 1:20).
This gave a mixed population of cells with 20 % expressing CD34, a
hematopoietic stem cell
marker, with some co-expression of endothelial cell
markers (CD31, Flk1, and VE - cadherin).
Hematopoietic stem cells express the
markers CD34 and CD133 and can repopulate a myeloablated recipient after allogeneic or autologous transplantation.
Finally, within the fractionated cell populations, there was evidence for coexpression of lineage specific
markers alongside of pluripotency genes, similar to lineage priming described first in
hematopoietic stem cells [15].