Binding and uptake of radiolabeled ApoC - III — depleted TRLs was significantly increased in
hepatocytes isolated from Ndst1fl / fl Alb - Cre + mice (i.e., in mice expressing both LDLR and LRP1) when compared with ApoC - III — bearing TRLs (Figure 10).
Binding and uptake of isolated [3H] TRLs (50 μg / ml) from control ASO - and ApoC - III ASO — treated Ldlr — / — Ndst1fl / fl Alb - Cre + mice in primary
hepatocytes isolated from Ndst1fl / fl Alb - Cre + and Ldlr — / — Lrp1fl / fl Alb - Cre — mice after a 4 - hour incubation at 37 °C (n = 3 / condition).
In a second approach, [3H] retinol - radiolabeled ApoC - III — depleted and ApoC - III — bearing TRLs (Figure 9A and Supplemental Figure 6) were evaluated for their uptake by primary
hepatocytes isolated from Ndst1fl / fl Alb - Cre + and Ldlr — / — Lrp1fl / fl Alb - Cre + mice.
Overall binding of both [3H] TRL preparations was significantly reduced in
hepatocytes isolated from Ldlr — / — Lrp1fl / fl Alb - Cre + mice compared with
hepatocytes isolated from Ndst1fl / fl Alb - Cre + mice (Figure 10).
Not exact matches
If the User requests
isolated cells, Users will provide an account number for a charge - back of $ 100 for each
hepatocyte / Kupffer cell isolation, to cover the cost of biochemicals, gradients and cell culture media required for isolation of purified cell populations.
Asialoglycoprotein receptor 1 is a specific cell - surface marker for
isolating hepatocytes derived from human pluripotent stem cells.
The
isolated primary
hepatocytes have limited proliferation potential and their quality and metabolic / functional activity vary.
Primary human
hepatocytes can be
isolated from non-transplantable or fetal livers, or liver resections.
I. Membrane - fixed IgG on
isolated hepatocytes from patients.Clinical and Experimental Immunology.