Our facility houses two third - generation Helios mass cytometers that can routinely analyze more than 40 markers per tube to provide an unprecedented «systems - level» view of phenotypic and functional variations among single cells within
heterogeneous cell populations.
High contrast images facilitate robust automatic tracking and behavioural analysis of hundreds of individual cells within
heterogeneous cell populations is now possible without the need for intrusive dyes or labels, bringing unique morphological, temporal, and dynamic phenotypic data to your research.
High - throughput single - cell RNA sequencing recently emerged as a powerful tool to profile complex and
heterogeneous cell populations.
Not exact matches
Once endothelial
cells are removed from a
heterogeneous population of
cells, it is often difficult to obtain a sufficient number of
cells for experimental analysis.
The postnatal adipose tissue contains a
heterogeneous population of
cells which includes adult stem
cells (i.e., MSCs), endothelial progenitor
cells, leukocytes, endothelial
cells, and vascular smooth muscle
cells.
Mass Cytometry is a next - generation flow cytometry technology that enables highly dimensional functional and phenotypic profiling of single
cells within
heterogeneous populations.
New CFSE - based assay to determine susceptibility to lysis by cytotoxic T
cells of leukemic precursor
cells within a
heterogeneous target
cell population.
Thus phenotypically «undifferentiated»
cells may consist of a
heterogeneous population of functionally distinct
cell types.
At this point, some colonies seemed unstable, with a strong tendency to spontaneously differentiate and form a
heterogeneous population of
cells (Fig. 2B, C).
Immunofluorescence analysis of the two
populations, following overnight adherence to exclude analysis of non-viable
cells, revealed that > 97 % of the SSEA3 - positive
population expressed detectable SSEA3 / 488 fluorescence and 0 % of the SSEA3 - negative
population expressed detectable SSEA3 / 488 fluorescence (Figure 2C), demonstrating that the fluorescence activated
cell sorting process can purify viable subpopulations of
cells from a
heterogeneous somatic
population.
To our knowledge, this study is the first to identify a pluripotency marker in a
heterogeneous population of human dermal fibroblasts, to isolate a subpopulation of
cells that have a significantly increased propensity to reprogram to pluripotency and to identify a possible mechanism to explain this differential reprogramming.
The tumor microenvironment is comprised of
heterogeneous populations of
cells including cancer, immune and tumor associated stromal
cells.
Satellite
cells (SCs) are a
heterogeneous population of stem and progenitor
cells that have been demonstrated to play a pivotal role in skeletal muscle (SkM) regeneration.