At this point, some colonies seemed unstable, with a strong tendency to spontaneously differentiate and form
a heterogeneous population of cells (Fig. 2B, C).
The postnatal adipose tissue contains
a heterogeneous population of cells which includes adult stem cells (i.e., MSCs), endothelial progenitor cells, leukocytes, endothelial cells, and vascular smooth muscle cells.
Once endothelial cells are removed from
a heterogeneous population of cells, it is often difficult to obtain a sufficient number of cells for experimental analysis.
The tumor microenvironment is comprised of
heterogeneous populations of cells including cancer, immune and tumor associated stromal cells.
Not exact matches
High contrast images facilitate robust automatic tracking and behavioural analysis
of hundreds
of individual
cells within
heterogeneous cell populations is now possible without the need for intrusive dyes or labels, bringing unique morphological, temporal, and dynamic phenotypic data to your research.
Mass Cytometry is a next - generation flow cytometry technology that enables highly dimensional functional and phenotypic profiling
of single
cells within
heterogeneous populations.
Our facility houses two third - generation Helios mass cytometers that can routinely analyze more than 40 markers per tube to provide an unprecedented «systems - level» view
of phenotypic and functional variations among single
cells within
heterogeneous cell populations.
New CFSE - based assay to determine susceptibility to lysis by cytotoxic T
cells of leukemic precursor
cells within a
heterogeneous target
cell population.
Thus phenotypically «undifferentiated»
cells may consist
of a
heterogeneous population of functionally distinct
cell types.
Immunofluorescence analysis
of the two
populations, following overnight adherence to exclude analysis
of non-viable
cells, revealed that > 97 %
of the SSEA3 - positive
population expressed detectable SSEA3 / 488 fluorescence and 0 %
of the SSEA3 - negative
population expressed detectable SSEA3 / 488 fluorescence (Figure 2C), demonstrating that the fluorescence activated
cell sorting process can purify viable subpopulations
of cells from a
heterogeneous somatic
population.
To our knowledge, this study is the first to identify a pluripotency marker in a
heterogeneous population of human dermal fibroblasts, to isolate a subpopulation
of cells that have a significantly increased propensity to reprogram to pluripotency and to identify a possible mechanism to explain this differential reprogramming.
Satellite
cells (SCs) are a
heterogeneous population of stem and progenitor
cells that have been demonstrated to play a pivotal role in skeletal muscle (SkM) regeneration.