Sentences with phrase «heterozygous cells»

Relative to XpdTTD / KO hemizygote cells, UV survival was improved by the homozygous lethal Xpd † XPCS allele in XpdTTD / † XPCS compound heterozygous cells and to a lesser degree by the Xpd † XP allele (Figure 4A).

Although both the mutant and the remaining normal allele were expressed equally, the heterozygous cells were not neoplastically transformed.

In the latest experiments the Mitalipov group focused on snipping out the mutated gene in heterozygous cells — a situation in which there was still a «good» nonmutated copy available for the natural cellular repair systems in the embryo to use as a template for repair after the researchers edited out the problematic one.

Not exact matches

Within the paired chromosomes contained in every cell, mutations occurring on one chromosome are known as heterozygous, while those occurring on both are homozygous.

In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells.

Furthermore, this study revealed that cells derived from humans can be grafted into the heterozygous inner ear of mouse embryos.

Most notably, the studies are based on transient transfection of wild - type and mutant gene constructs into cultured cells or into zebrafish embryos and are unlikely to reproduce the specific mutant gene dose that is present in heterozygous mutant cells in FOP patients.

Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.

Next we asked whether the Xpd † XPCS and Xpd † XP alleles, despite decreased mRNA levels, ameliorated TTD symptoms by increasing overall TFIIH levels in compound heterozygous XpdTTD / † XPCS and XpdTTD / † XP cells.

Although interallelic complementation between two endogenous mutant alleles has been described in cells from a compound heterozygous patient with methylmalonic acidaemia, no observable effects on disease outcome were noted in the patient [28].

(E) Xpd dose - dependent reduction of TFIIH in homozygous XpdTTD / TTD, hemizygous XpdTTD / KO, and compound heterozygous XpdTTD / † XPCS and XpdTTD / † XP cells by comparative immunofluorescence of the p62 subunit of TFIIH.

mRNA expression from the targeted allele could be detected in embryonic stem cells by RT - PCR (Figure 1D), although expression was reduced approximately 5-fold relative to wt mRNA transcript levels as determined by Northern blotting of RNA from the testis of heterozygous animals (Figure 1E).

Assuming that all mutations are heterozygous (which is likely), the observed allele frequencies suggest that shared mutations are present in virtually all tumor cells.

A heterozygous founder mutation, for example, would be present in virtually all tumor cells and have a mutation frequency of 50 % in diploid cells.

It was heterozygous in most patients, homozygous in a subset as a result of mitotic recombination, and arose in a multipotent progenitor capable of giving rise to erythroid and myeloid cells.

The service provided covers the production of a minimum of two heterozygous mice carrying the targeted gene allele of choice from the corresponding validated gene - targeted ES cell clone (s).

We also found that both ILP2 and ILP5 accumulated in the insulin - producing cells of gbp1, gbp2 null mutant larvae when compared to wild - type or heterozygous mutant animals (Fig 4E — 4H).

To confirm whether ILP accumulation in the insulin - producing cells actually results in a decrease of ILP2 concentration in the circulating hemolymph, we collected hemolymph from wild - type, and gbp1, gbp2 ex67 heterozygous and homozygous mutant larvae at 24 h AL3E, and performed western blot analyses using an anti-ILP2 antibody.

Differentiation of this heterozygous iPSC line to erythrocytes used an improved feeder - free and xeno - free three - step culture strategy, generating enucleated hemoglobin expressing cells to a similar level to that observed for the parental TNC1 iPSC line (see figure).