Sentences with phrase «hips cell clones»

Thus, these data show that the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System effectively edits and expands single - cell hiPS cell clones without introducing karyotypic abnormalities.

Pluripotency was maintained in edited hiPS cell clones that were seeded as single cells.

hiPS cell clones that have been edited via gesicles maintain a stable karyotype after editing and clonal expansion.

Graphical representation of indels created at the CD81 target site in different CRISPR / Cas9 - edited hiPS cell clones.

Not exact matches

This kit utilizes the DEF ‑ CS culture system, recognized for its suitability for genome engineering (Valton et al. 2014) and single - cell cloning (Feng et al. 2014), to promote reliable growth of hiPS cells in a feeder - free and defined environment.

Workflow for targeted knockout of CD81, an endogenous gene in hiPS cells, using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

These data demonstrate that the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System enables highly efficient survival of single hiPS cells.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Cell Cloning DEF - CS Culture Media Kit:

Single - cell seeding of edited hiPS cells in this system results in high survival of pluripotent, edited clones, yielding a diverse set of edited clonal lines.

The Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System provides an efficient and effective method to generate clonal lines of edited hiPS cells.

hiPS cells can be cultured, edited, and clonally expanded using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

CRISPR / Cas9 - mediated editing and single - cell cloning can be harsh on hiPS cells.

After gesicle - based delivery of the editing machinery into hiPS cells, cells must survive the process of single - cell cloning — a major bottleneck to obtaining clones with the modification of interest.

The sequential processes of somatic cell reprogramming to create patient - specific hiPS cells, CRISPR / Cas9 gene editing, and single - cell cloning uniquely enable researchers to study how a specific genetic modification can influence function.