Sentences with word «homogenate»

Whole genome DNA was isolated from brain and meningeal homogenate using DNeasy Blood and Tissue Kit (Qiagen).

Associate Professor Kunihiro Sakuma and his colleagues at Toyohashi Tech found a marked accumulation of p62 / SQSTM1 in the sarcopenic quadriceps muscle of mice using two different methods (western blotting of cell - fractionated homogenates and immunofluorescence).

However, all these studies involved only western blotting analyses of crude not cell - fractionated muscle homogenates.

Injection of estrogens (17β - estradiol or diethylstilbestrol) into immature chicks results in a marked (30 - to 50-fold) increase in the ornithine decarboxylase activity of oviductal homogenates within a 4 - hour period.

* Systematic Bias Introduced by Genomic DNA Template Dilution in 16S rRNA Gene - Targeted Microbiota Profiling in Human Stool Homogenates — Francesco Multinu — mSphere

This antagonist inhibited IP3 evoked Ca2 + release in sea urchin homogenate and oxytocin induced contraction in whole uterine strips.

[A] Quantitative PCR of genomic DNA prepared from 10 % of brain homogenate reveals that parasite DNA was readily detectable in Type III - infected animals (i) but undetectable in attenuated Type I - infected animals (ii).

TBS - soluble a) Aβ40 and b) Aβ42 levels in cortex + hippocampus homogenates from non-transgenic -LRB-- / --RRB-, heterozygous (+ / --RRB- and homozygous transgenic (+ / +) rats at different ages (3 months, 7 months and 13 — 15 months) were quantified with specific G2 - 10 / W0 - 2 and G2 - 13 / W0 - 2 sandwich ELISAs, respectively.

The remaining homogenate was digested in 1 mg / mL Collagenase IA (Sigma) and 0.1 mg / mL DNase I (Roche) for 40 minutes at 37 °C.

Liver homogenate was incubated with 100 U / ml collagenase (Sigma; St. Louis, MO) and 20 μg / ml DNase I (Sigma) for 40 min at 37 °C.

Western blot of whole rat cerebellum homogenate stained with ab7260 at dilution of 1:100,000.

We also infected mice with attenuated Type I parasites and detected parasites in the brain and meningeal homogenate in a number of mice between 5 and 20 days post infection (Figure 3D).

Immunodepletion of IAPP from 1 % islet homogenate of overtly diabetic Tg - hIAPP mice was performed in three successive rounds, using Dynabeads (Novex 11203D; Thermo Fisher Scientific) coupled with a cocktail of antibodies.

It has not been validated on blood samples from people with vCJD, because these have not been made available, says Moser — but the test can detect highly diluted vCJD brain homogenates, which contain the prion, in blood.

An adenylate cyclase that is activated specifically by very low concentrations of octopamine has been identified both in homogenates and in intact cells of the thoracic ganglia of an insect nervous system.

A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver.

To rule out that the staining was coming from the aggregates present in the inoculum, cultured islets from WT animals, which do not express hIAPP, were incubated with the same amount of old Tg islet homogenate.

(E and F) Black bars represent the animals inoculated with old Tg - hIAPP pancreas homogenate, and white bars correspond to the controls injected with WT pancreas.