Sentences with phrase «human t cells»

Citation: Provençal N, Suderman MJ, Guillemin C, Vitaro F, Côté SM, Hallett M, et al. (2014) Association of Childhood Chronic Physical Aggression with a DNA Methylation Signature in Adult Human T Cells.

In a study published in Molecular & Cellular Proteomics, investigators at Harvard and Stanford universities led by Christina Woo employed a method called IsoTaG to catalogue and quantify the O - GlcNAc sites in resting and activated human T cells.

Epigenomic analysis of primary human T cells reveals novel enhancers associated with Th2 memory differentiation and asthma susceptibility.

CRISPR / Cas9 was able to eliminate HIV in infected human T cells in culture.

For the study, the researchers treated part of a human artery — a few millimeters in diameter — with the siRNA - loaded nanoparticles and transplanted it into the abdominal aorta of an immune - deficient mouse inoculated with human T cells.

Cytokine modulation of TH1 / TH2 phenotype differentiation in directly alloresponsive CD4 + human T cells.

Effective gene suppression using small interfering RNA in hard - to - transfect human T cells.

Last year, Marson and Schumann successfully used CRISPR to perform precise DNA sequence replacements in primary human T cells for the first time by prefabricating the CRISPR machinery in test tubes, then adding it to the freshly donated immune cells.

Mouse genes that make human T cells powerful at fighting liver cancer could one day help patients do the same, scientists report.

The mouse T cells developed receptors for this clearly suspicious invader and the genes for the receptors is what He later put on human T cells.

To determine the efficiency and specificity with which the cxcr4 genes could be disrupted in human T cells, we produced a bicistronic Ad5 / F35 vector to deliver the X4 - ZFNs (AdX4 - ZFNs).

Previously we engineered zinc - finger nucleases (ZFNs) to specifically disrupt the CCR5 gene in primary human T cells, the predominant cell type infected and killed by HIV.

Here we describe engineering a pair of zinc finger nucleases that, when introduced into human T cells, efficiently disrupt cxcr4 by cleavage and error - prone non-homologous DNA end - joining.

The scientists took the genes for the most effective liver cancer antigen receptors on those T cells, put those receptors on human T cells and the resulting engineered human T cells eradicated the cancer as well, without hurting normal liver cells, they report in the journal Hepatology.

If the engineered human T cells do produce a robust cancer attack — and don't generate toxicity by also attacking healthy cells — the next step would be clinical trials, He says.

In the humanized mouse model under the conditions studied here, partial loss of cxcr4 in human T cells due to treatment with X4 - ZFNs provided selective pressure for either the evolution or emergence of a pre-existing single amino acid mutation in the V3 loop of the infecting X4 HIV - 1 strain that enabled it to use CCR5 as efficiently as CXCR4.

Until recently, however, editing human T cells with CRISPR / Cas9 has been inefficient, with only a relatively small percentage of cells being successfully modified.

«Genome editing in human T cells has been a notable challenge for the field,» Marson said.

«In CRISPR advance, scientists successfully edit human T cells: Research has implications for autoimmune diseases, AIDS, cancer.»

To overcome this hurdle, researchers genetically engineered human T cells to produce a CAR protein that recognizes a glycopeptide found on various cancer cells but not normal cells, and then demonstrated its effectiveness in mice with leukemia and pancreatic cancer.

Using human T cells in culture as well as mice lacking one of these genes, YY1, they found that, indeed, the two proteins did help move Xist back to the inactive X chromosome in activated lymphocytes.

Because human T cells don't have as many of these brakes, our cells are a hundred times more aggressive than those of chimps when faced with drugs like TGN1412, which work by triggering the immune system.

«These animals carry latent HIV in the genomes of human T cells, where the virus can escape detection,» Dr. Hu explained.

«We studied human T cells, isolated from blood donors of all ages, to compare mature cytotoxic T cells with naive ones,» said Philip Ansumana Hull, graduate student in Ott's lab and one of the first authors of the study.

In Britain, in 1991, researchers led by Jim Stott at the National Institute of Biological Standards and Control in north London, stunned their colleagues by announcing that they had apparently protected monkeys from infection with the monkey virus SIV — the simian equivalent of HIV — with a vaccine based simply on human T cells.

«In a single dose, APC - mimetic scaffolds led to two - to ten-fold greater expansion of primary mouse and human T cells than Dynabeads.

Like the Rosetta Stone that scholars used to decode hieroglyphics, researchers trained the algorithm with more than 4,600 T cell receptors and then used it to correctly assign 81 percent of the human T cells and 78 percent of mouse T cells to one of 10 different viral epitopes.

To estimate the prevalence of positive serology among potential donors to a human milk bank.Retrospective review of our experience with donor serological testing at our milk bank over a 6 - year interval.Not - for - profit, regional human milk bank.Volunteer, unpaid potential donors of human milk.Serological testing for syphilis, HIV, hepatitis B, hepatitis C, human T cell lymphotropic virus type 1 (HTLV - 1) and human T cell lymphotropic virus type 2 (HTLV - 2).

He is part of the team that cloned the gene for the human T cell receptor in 1984, which he describes as «the Holy Grail of immunology.»

Fusion product of human B cell line 721.174 and human T cell line CEM.

Characterization of a nuclear export signal within the human T cell leukemia virus type I transactivator protein Tax.

Secretion of the human T cell leukemia virus type I transactivator protein tax.

Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein.

We present an in - depth characterization of human T cell populations using a panel of ~ 80 antibodies revealing complexity not previously observed by scRNA - seq alone.

Prof. Caccamo's research is focused on the role of human T cell subsets in physiology and in pathology, on the role of human T lymphocytes during M. tuberculosis infection and on the identification, optimization and evaluation of correlates of protection and tuberculosis disease.

The human T cell receptor beta (TRB, previously known as TCRB) gene locus on chromosome 7 (7q34) includes 65 Vb (variable) gene segments, followed by two separate clusters of genes each containing a Db (diversity) gene, several Jb (joining) genes, and a Cb (constant) region spread over 685 kilobases.

Research Focus: My research project is focused on the characterization of human T cell immune responses against Dengue virus.

These efforts have resulted in the discovery of previously unknown human T cell epitopes that may be key in the initiation of allergic responses, and to the development of biochemical and bioinformatic tools that have been used to map the human T cell response to a large panel of common allergens.

High - resolution maps of enhancer - promoter interactions in rare primary human T cell subsets and coronary artery smooth muscle cells link variants associated with autoimmune and cardiovascular diseases to target genes.

Not exact matches

For example, CRAC channels in T cells have been clinically validated as important drug targets through human mutations and the use of calcineurin inhibitors that act downstream from CRAC channels.

Hoping to learn something about how the human body defends itself against cancer, he had zeroed in on a complex regiment of lymphocytes called T cells, common to the immune systems in both mouse and man.

Some of these risks include the presence of cytomegalovirus (CMV), hepatitis, human immunodeficiency virus (HIV), human T - cell leukemia virus type 1 (HTLV - 1) or other viruses on the pump parts that were exposed to the previous mother's breastmilk.

Potential donors must also have blood drawn to test for HIV, HTLV (human T - cell lymphotropic virus), Syphilis, Hepatitis B, and Hepatitis C.

Some of the viruses that can be within breast milk are: HIV — Human Immunodeficiency Virus (AIDS) HTLV - 1 Human T - Cell Leukemia Virus Type I CMV — Cytomegalovirus When you are using a previously owned breast pump you create the risk of cross contamination.

Certain infections, like HIV; untreated, active tuberculosis (ok to pump); untreated brucellosis; active herpes lesions on her breast (ok to pump); and mothers who are positive for the human T - cell lymphotropic virus type I or II

Also, women with human T - cell leukemia virus type 1 (HTLV - 1) should not breastfeed because of the risk of transmission to the child.

Donors should be tested for HIV 1 and 2, Human T - cell lymphotropic virus or HTLV 1 and 2, syphilis, and hepatitis B and C.

Breastfeeding is contraindicated in infants with classic galactosemia (galactose 1 - phosphate uridyltransferase deficiency) 103; mothers who have active untreated tuberculosis disease or are human T - cell lymphotropic virus type I — or II — positive104, 105; mothers who are receiving diagnostic or therapeutic radioactive isotopes or have had exposure to radioactive materials (for as long as there is radioactivity in the milk) 106 — 108; mothers who are receiving antimetabolites or chemotherapeutic agents or a small number of other medications until they clear the milk109, 110; mothers who are using drugs of abuse («street drugs»); and mothers who have herpes simplex lesions on a breast (infant may feed from other breast if clear of lesions).

Don't breastfeed if you have untreated, active tuberculosis, or human T - cell lymphotropic virus Type 1or Type 2.

The research involved using novel technologies to examine the T cells in human tissues.